胡芦巴生物活性物质 Trigonelline 可保护心脏组织免受酒精中毒：以抗氧化角度为重点的体内研究。

IF 1.7 Q3 INTEGRATIVE & COMPLEMENTARY MEDICINE Journal of Ayurveda and Integrative Medicine Pub Date : 2024-07-01 DOI:10.1016/j.jaim.2024.100963

Meesala Guru Sekhar , Kondeti Ramudu Shanmugam , Inavolu Srinivasa Chakrapani

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引用次数: 0

摘要

背景介绍Trigonella foenum-graecum，俗称葫芦巴，是一种香料。它具有抗氧化、抗糖尿病、降血脂和其他药理特性：研究的目的是检测 Trigonelline（TG）对酒精中毒大鼠心脏的保护作用，TG 是 Trigonella foenum-graecum（TF）的一种生物活性化合物：将年轻的 Wistar 白化大鼠分为 5 组，并按照实验方案进行治疗。评估所有实验组心脏组织中的抗氧化酶、超氧化物歧化酶（SOD）、谷胱甘肽过氧化物酶（GPx）、过氧化氢酶（CAT）、谷胱甘肽还原酶（GR）、谷胱甘肽（GSH）、丙二醛（MDA）水平。还估测了心脏标志物肌酸激酶-MB（CK-MB）、肌钙蛋白-T（TT）、肌钙蛋白-I（TI）、肌红蛋白（MG）和血清标志物丙氨酸转氨酶（AAT）、天门冬氨酸转氨酶（AST）和碱性磷酸酶（ALP）。此外，还对三叶草乙醇提取物的自由基清除活性（如 2,2-二苯基吡啶肼（DPPH）、过氧化氢（H2O2）和羟自由基）进行了评估：结果：酒精中毒大鼠的 SOD、CAT、GPx、GR、GSH 活性降低，MDA、CK-MB、TT、TI、MG 和 AAT、AST、ALP 活性升高。给酒精中毒的大鼠补充 30 天的川芎苷可提高抗氧化酶的活性，降低 MDA、心脏指标和血清指标。自由基清除试验也表明，川芎具有清除自由基的活性。此外，我们的组织病理学证据也证明，TG 能保护所有实验鼠的心脏组织免受酒精引起的毒性：我们的研究得出结论，麦角茶可能对酒精中毒和心肌梗塞患者有用。

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Trigonelline, a Fenugreek Bioactive protects Heart tissue against alcohol intoxication: An in-vivo study focusing on antioxidant perspective

Background

Trigonella foenum-graecum, commonly known as fenugreek and it is used as a spice. It has antioxidant, anti-diabetic, antilipedemic and other pharmocological properties.

Objectives

The aim of the study was to detect the cardio protective activity of Trigonelline (TG) a bioactive compound of Trigonella foenum-graecum (TF) in alcohol intoxicated rats.

Material and methods

The young wistar strain albino rats are divided in to 5 groups and treatment was given as per the experimental protocol. Antioxidant enzymes, superoxide dismutase (SOD), glutathione peroxidase (GPx), catalase (CAT), glutathione reductase (GR), glutathione (GSH), malondialdehyde (MDA) levels are estimated in cardiac tissue of all experimental groups. Cardiac markers creatine kinase-MB (CK-MB), troponin-T (TT), troponin-I (TI), myoglobin (MG) and serum markers alanine transaminase (AAT), aspartate transaminase (AST) and alkaline phosphatase (ALP) are estimated. Free radical scavenging activities like 2,2-diphenylpicrylhydrazyl (DPPH), hydrogen peroxide (H₂O₂) and hydroxyl radical are estimated in ethanolic extract of Trigonella foenum-graecum.

Results

SOD, CAT, GPx, GR, GSH activities are depleted and MDA, CK-MB, TT, TI, MG and AAT, AST, ALP activities are elevated in alcohol intoxicated rats. Trigonelline supplementation to alcoholic rats for 30 days elevated antioxidant enzymes, depleted MDA, cardiac markers and serum markers in alcohol intoxicated rats. Free radical scavenging assay also reported that Trigonella foenum-graecum possess free radical scavenging activity. Furthermore, our histopathological evidence also proved that TG protected the cardiac tissue from alcohol induced toxicity in all the experimental rats.