导致印度尼西亚芒果枯萎病的 Colletotrichum spp.

IF 1.8 3区 农林科学 Q2 PLANT SCIENCES Plant Pathology Journal Pub Date : 2024-08-01 DOI:10.5423/PPJ.NT.04.2024.0061
Khaerani Nurlaelita, Arif Wibowo, Ani Widiastuti
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引用次数: 0

摘要

在印度尼西亚,特别是爪哇岛,发现了芒果树的枯萎病,但病原菌仍未确定。Colletotrichum 是导致芒果枯萎病的重要病原体之一。在印度尼西亚爪哇岛的多个芒果种植区进行了实地调查,以评估枯萎病病原体 Colletotrichum 的流行情况。从有症状的枯萎病树枝上分离出 11 株 Colletotrichum,并对其进行了形态鉴定。使用 rep-PCR 进行了遗传多样性指纹分析。利用四个基因区的部分序列,包括 ITS、ACT、GAPDH 和 TUB2,通过系统发育分析确定分离株属于 Colletotrichum asianum 和 Colletotrichum cairnsense。对 Arumanis 品种的芒果幼苗进行的致病性测试表明,所有真菌分离物都能引起枯萎症状。随后,对有症状的组织进行了重新分离,以验证科赫推定。这项研究为印尼两种引起芒果枯萎病的 Colletotrichum 提供了新的资金来源。
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Identification and Characterization Colletotrichum spp. Causing Mango Dieback in Indonesia.

Dieback disease in mango trees has been observed in Indonesia, particularly in Java Island, with the causal agent remaining unidentified. One of the important pathogens that are responsible for causing mango dieback is Colletotrichum. Field surveys were conducted in various mango cultivating areas in Java Island, Indonesia to assess prevalence of Colletotrichum as dieback disease pathogen. Eleven Colletotrichum isolates were recovered from symptomatic dieback twigs and morphologically characterized. Genetic diversity fingerprint analysis was carried out using rep-PCR. Phylogenetic analysis identified isolates as belonging to Colletotrichum asianum and Colletotrichum cairnsense using partial sequences of four gene regions, including ITS, ACT, GAPDH, and TUB2. Pathogenicity tests on mango seedlings cv. Arumanis showed that all fungal isolates were responsible for causing dieback symptoms. Subsequently, symptomatic tissue was reisolated to fulfill Koch's Postulate. This study represented new funding for two species of Colletotrichum causing mango dieback in Indonesia.

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来源期刊
Plant Pathology Journal
Plant Pathology Journal 生物-植物科学
CiteScore
4.90
自引率
4.30%
发文量
71
审稿时长
12 months
期刊介绍: Information not localized
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