{"title":"铁(III)介导的微藻相关细菌群落和溶解有机物特征的变化:关于 Chlorococcum sp.","authors":"","doi":"10.1016/j.algal.2024.103637","DOIUrl":null,"url":null,"abstract":"<div><p>Trivalent iron ions (Fe(III)) have important effects on aquatic ecosystems, especially on the functional diversity and stability of microorganisms and ecosystems. Here, a cultivable microalgae (<em>Chlorococcum</em> sp. GD) from the natural environment (Shanxi, China) were isolated and identified. A combined high-throughput 16S rRNA gene amplicon sequencing/excitation-emission matrix coupled with parallel factor (EEM-PARAFAC) analysis was used to integrated the results of Fe(III) influence on <em>Chlorococcum</em> sp. GD bacterial community and dissolved organic matter (DOM) characteristics through laboratory experiments. Due to the addition of Fe(III) in the form of ferric nitrate (Fe(NO<sub>3</sub>)<sub>3</sub>·9H<sub>2</sub>O), complexed with EDTA to maintain solubility and bioavailability, the bacterial community was altered. This led to a decrease in the relative abundance of Proteobacteria and Actinobacteria and an increase in the relative abundance of Cyanobacteria, especially under excessive Fe(III) treatment. In addition, the relative contribution of bacterial community dispersal limitation and homogenizing dispersal was 100 %, which may lead to differences in their local adaptation and ecological processes with homogenization of bacterial diversity and loss of function. Excessive Fe(III) caused a significant increase in the abundance of genes involved in the carbon cycle (<em>p</em> < 0.01) and a significant decrease in genes involved in the nitrogen cycle (<em>p</em> < 0.01), further affecting the overall regulatory network of gene expression. This led to an increase in the abundance of genes involved in metabolism, cellular processes, environmental information processing, genetic information processing, and human diseases. The moderate amount of Fe(III) promoted the production of microbial components and accelerated the degree of DOM humification. It is also worth mentioning that excessive Fe(III) inhibited DOM degradation. Overall, this work explored the characteristics of bacterial community and DOM changes in Fe(III)-stressed <em>Chlorococcum</em> sp. GD as an example, which contributes to an in-depth understanding of microbial community diversity and element cycling in aquatic ecosystems.</p></div>","PeriodicalId":7855,"journal":{"name":"Algal Research-Biomass Biofuels and Bioproducts","volume":null,"pages":null},"PeriodicalIF":4.6000,"publicationDate":"2024-08-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":"0","resultStr":"{\"title\":\"Fe(III)-mediated changes in microalgae-associated bacterial communities and dissolved organic matter characteristics: A case study for Chlorococcum sp. 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This led to a decrease in the relative abundance of Proteobacteria and Actinobacteria and an increase in the relative abundance of Cyanobacteria, especially under excessive Fe(III) treatment. In addition, the relative contribution of bacterial community dispersal limitation and homogenizing dispersal was 100 %, which may lead to differences in their local adaptation and ecological processes with homogenization of bacterial diversity and loss of function. Excessive Fe(III) caused a significant increase in the abundance of genes involved in the carbon cycle (<em>p</em> < 0.01) and a significant decrease in genes involved in the nitrogen cycle (<em>p</em> < 0.01), further affecting the overall regulatory network of gene expression. This led to an increase in the abundance of genes involved in metabolism, cellular processes, environmental information processing, genetic information processing, and human diseases. The moderate amount of Fe(III) promoted the production of microbial components and accelerated the degree of DOM humification. It is also worth mentioning that excessive Fe(III) inhibited DOM degradation. Overall, this work explored the characteristics of bacterial community and DOM changes in Fe(III)-stressed <em>Chlorococcum</em> sp. 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引用次数: 0
摘要
三价铁离子(Fe(III))对水生生态系统有重要影响,尤其是对微生物和生态系统的功能多样性和稳定性。本文从自然环境(中国山西)中分离并鉴定了一种可栽培的微藻(GD藻)。采用高通量 16S rRNA 基因扩增片段测序/激发-发射矩阵耦合并行因子(EEM-PARAFAC)分析方法,通过实验室实验综合分析了 Fe(III)对 GD 藻细菌群落和溶解有机物(DOM)特征的影响。由于添加了硝酸铁(Fe(NO)-9HO)形式的铁(III),并与乙二胺四乙酸(EDTA)络合以保持溶解度和生物利用度,细菌群落发生了变化。这导致变形菌和放线菌的相对丰度下降,而蓝藻的相对丰度上升,尤其是在过量的铁(III)处理条件下。此外,细菌群落扩散限制和同质化扩散的相对贡献率为 100%,这可能会导致细菌多样性同质化和功能丧失,从而导致它们在当地适应和生态过程中的差异。过量的 Fe(III)导致参与碳循环的基因丰度显著增加(< 0.01),参与氮循环的基因丰度显著减少(< 0.01),进一步影响了基因表达的整体调控网络。这导致参与新陈代谢、细胞过程、环境信息处理、遗传信息处理和人类疾病的基因数量增加。适量的铁(III)促进了微生物成分的产生,加快了 DOM 的腐殖化程度。值得一提的是,过量的铁(III)会抑制 DOM 的降解。总之,本研究探索了铁(III)胁迫藻类中细菌群落的特征和 DOM 的变化。以 GD 为例,有助于深入了解水生生态系统中微生物群落多样性和元素循环。
Fe(III)-mediated changes in microalgae-associated bacterial communities and dissolved organic matter characteristics: A case study for Chlorococcum sp. GD
Trivalent iron ions (Fe(III)) have important effects on aquatic ecosystems, especially on the functional diversity and stability of microorganisms and ecosystems. Here, a cultivable microalgae (Chlorococcum sp. GD) from the natural environment (Shanxi, China) were isolated and identified. A combined high-throughput 16S rRNA gene amplicon sequencing/excitation-emission matrix coupled with parallel factor (EEM-PARAFAC) analysis was used to integrated the results of Fe(III) influence on Chlorococcum sp. GD bacterial community and dissolved organic matter (DOM) characteristics through laboratory experiments. Due to the addition of Fe(III) in the form of ferric nitrate (Fe(NO3)3·9H2O), complexed with EDTA to maintain solubility and bioavailability, the bacterial community was altered. This led to a decrease in the relative abundance of Proteobacteria and Actinobacteria and an increase in the relative abundance of Cyanobacteria, especially under excessive Fe(III) treatment. In addition, the relative contribution of bacterial community dispersal limitation and homogenizing dispersal was 100 %, which may lead to differences in their local adaptation and ecological processes with homogenization of bacterial diversity and loss of function. Excessive Fe(III) caused a significant increase in the abundance of genes involved in the carbon cycle (p < 0.01) and a significant decrease in genes involved in the nitrogen cycle (p < 0.01), further affecting the overall regulatory network of gene expression. This led to an increase in the abundance of genes involved in metabolism, cellular processes, environmental information processing, genetic information processing, and human diseases. The moderate amount of Fe(III) promoted the production of microbial components and accelerated the degree of DOM humification. It is also worth mentioning that excessive Fe(III) inhibited DOM degradation. Overall, this work explored the characteristics of bacterial community and DOM changes in Fe(III)-stressed Chlorococcum sp. GD as an example, which contributes to an in-depth understanding of microbial community diversity and element cycling in aquatic ecosystems.
期刊介绍:
Algal Research is an international phycology journal covering all areas of emerging technologies in algae biology, biomass production, cultivation, harvesting, extraction, bioproducts, biorefinery, engineering, and econometrics. Algae is defined to include cyanobacteria, microalgae, and protists and symbionts of interest in biotechnology. The journal publishes original research and reviews for the following scope: algal biology, including but not exclusive to: phylogeny, biodiversity, molecular traits, metabolic regulation, and genetic engineering, algal cultivation, e.g. phototrophic systems, heterotrophic systems, and mixotrophic systems, algal harvesting and extraction systems, biotechnology to convert algal biomass and components into biofuels and bioproducts, e.g., nutraceuticals, pharmaceuticals, animal feed, plastics, etc. algal products and their economic assessment