揭示茉莉酸盐介导的植物对桃绿蚜(Myzus persicae)的免疫力:一种综合转录组学和 RNA 测序方法

IF 3.5 3区 生物学 Q1 PLANT SCIENCES Plant Growth Regulation Pub Date : 2024-08-05 DOI:10.1007/s10725-024-01198-y
Xianyou Wang, Yi Zeng, Li Li, Rongyao Fan, Yujun Yan, Longlong Guo, Ruijin Zhou
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引用次数: 0

摘要

绿桃蚜(GPA)被认为是最具破坏性的害虫之一,对桃树(Prunus persica)的生长和果实质量构成严重威胁。长非编码 RNA(lncRNA)是一类重要的内源 RNA,在植物中发挥基因调控作用。在这项研究中,我们采用高通量链特异性 RNA 测序技术,从健康和受 GPA 侵染的柿子组织中鉴定出了 1776 个 lncRNA。我们对差异基因表达进行了严格分析,得出了 2871 个差异表达基因(DEGs),其中 1803 个基因表现出上调,1068 个基因表现出下调,以应对桃树中 GPA 的存在。我们的研究结果揭示了 lncRNAs 作为关键的 microRNA(miRNA)靶标的潜力,从而对 miRNA 的活性产生重大影响。我们进一步预测了两个与茉莉酸(JA)通路相关的差异表达 lncRNA-DEG 对(circ16-miR482a 和 circ116-miR319a)。值得注意的是,桃树中的内源 JA 水平不断被诱导,主要是作为一种抵抗 GPA 侵染的机制。此外,喷施 JA 能显著减少 GPA 的数量。
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Unraveling jasmonate-mediated plant immunity against green peach aphid (Myzus persicae) in peach: an integrated transcriptomic and RNA sequencing approach

The green peach aphid (GPA) is considered one of the most destructive pests posing a significant threat to the growth and fruit quality of peach trees (Prunus persica). Long non-coding RNAs (lncRNAs) represent an essential group of endogenous RNAs that play gene regulatory roles in plants. In this study, we identified 1776 lncRNAs from healthy and GPA-infested P. persica tissues, employing high-throughput strand-specific RNA sequencing. Our rigorous analysis of differential gene expression yielded 2871 differentially-expressed genes (DEGs), with 1803 genes exhibiting upregulation and 1068 genes exhibiting downregulation in response to the presence of GPA in peach trees. Our findings reveal the potential of lncRNAs to serve as crucial microRNA (miRNA) targets, thereby exerting a significant influence on miRNA activity. We further predicted two differentially expressed lncRNA–DEG pairs (circ16–miR482a and circ116–miR319a) associated with jasmonic acid (JA) pathway. Notably, endogenous JA levels in peach trees were continuously induced, primarily as a resistance mechanism against GPA infestation. Furthermore, spray application of JA significantly curtailed the GPA population.

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来源期刊
Plant Growth Regulation
Plant Growth Regulation 生物-植物科学
CiteScore
6.90
自引率
9.50%
发文量
139
审稿时长
4.5 months
期刊介绍: Plant Growth Regulation is an international journal publishing original articles on all aspects of plant growth and development. We welcome manuscripts reporting question-based research using hormonal, physiological, environmental, genetical, biophysical, developmental or molecular approaches to the study of plant growth regulation. Emphasis is placed on papers presenting the results of original research. Occasional reviews on important topics will also be welcome. All contributions must be in English.
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