量化细菌中 cAMP 信息传递的第二信使信号的最佳频率

Jiarui Xiong, Liang Wang, Jialun Lin, Lei Ni, Rongrong Zhang, Shuai Yang, Yajia Huang, Jun Chu, Fan Jin
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引用次数: 0

摘要

细菌第二信使对于将环境信息传递给细胞反应至关重要。然而,量化它们的信息传递能力仍然具有挑战性。在这里,我们利用定向基因敲除、光遗传学和荧光 cAMP 探针,在铜绿假单胞菌中设计了一个孤立的 cAMP 信号通道。这种设计允许对 cAMP 动态进行精确的光学控制和实时监测。通过将实验数据与信息理论相结合,我们揭示了光介导的 cAMP 信号传递的最佳频率,它能最大限度地传输信息,达到约 40 比特/小时。这一频率与 cAMP 降解动力学密切相关,并采用了双态编码方案。我们的发现提出了一种通过第二信使信号的时间编码对多个基因进行微调的机制,为细菌的适应策略提供了新的见解。
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Optimal Frequency in Second Messenger Signaling Quantifying cAMP Information Transmission in Bacteria
Bacterial second messengers are crucial for transmitting environmental information to cellular responses. However, quantifying their information transmission capacity remains challenging. Here, we engineer an isolated cAMP signaling channel in Pseudomonas aeruginosa using targeted gene knockouts, optogenetics, and a fluorescent cAMP probe. This design allows precise optical control and real-time monitoring of cAMP dynamics. By integrating experimental data with information theory, we reveal an optimal frequency for light-mediated cAMP signaling that maximizes information transmission, reaching about 40 bits/h. This rate correlates strongly with cAMP degradation kinetics and employs a two-state encoding scheme. Our findings suggest a mechanism for fine-tuned regulation of multiple genes through temporal encoding of second messenger signals, providing new insights into bacterial adaptation strategies. This approach offers a framework for quantifying information processing in cellular signaling systems.
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