{"title":"利用 LC-QTOF-MS 鉴别鱼腥草叶片中的生物活性化合物对粘毛蓟马和禾谷圆线虫的杀线虫活性","authors":"","doi":"10.1016/j.cropro.2024.106875","DOIUrl":null,"url":null,"abstract":"<div><p><em>Hirschmanniella mucronata</em> and <em>Meloidogyne graminicola</em> are the main plant-parasitic nematodes found in paddy fields in Thailand causing significant rice production yield losses. Although these nematodes are found widely in fields, an effective management method has not been documented. Here, we examined the nematicidal effects of different cavalcade leaf ages (1-, 2- and 3-month-old), used as aqueous extracts and soil amendments, on <em>H. mucronata</em> and <em>M. graminicola</em>. <em>In vitro</em> tests evidenced maximum mortality of <em>H. mucronata</em> (55.6–60.0%) in 50 mg ml<sup>−1</sup> extract from all leaf ages, while mortality (65.9%) and hatchability (67.0%) of <em>M. graminicola</em> second-stage juveniles were observed in 50 mg ml<sup>−1</sup> extract from 1-month-old leaves only. Moreover, aqueous extracts of cavalcade showed a repellent effect on both nematodes. Similarly, greenhouse experiments showed a significant reduction of <em>H. mucronata</em> population densities and reproduction of <em>M. graminicola</em> in soil amended with 0.5 and 1.0% (w/w) from each leaf age. LC-QTOF-MS analysis determined that three bioactive compounds, including quercetin, rutin, and kaempferol, are associated with the nematicidal activity of cavalcade against <em>H. mucronata</em> and <em>M. graminicola</em>. The information derived from this study indicates that the leaves of cavalcade are a source of promising phytonematicidals. This is the first study to assess and document its potential for nematicidal activity against <em>H. mucronata</em> and <em>M. graminicola</em> in Thailand. However, further research is needed to evaluate their efficacy under paddy field conditions.</p></div>","PeriodicalId":10785,"journal":{"name":"Crop Protection","volume":null,"pages":null},"PeriodicalIF":2.5000,"publicationDate":"2024-08-02","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":"0","resultStr":"{\"title\":\"Identification of bioactive compounds in cavalcade leaves for nematicidal activity against Hirschmanniella mucronata and Meloidogyne graminicola using LC-QTOF-MS\",\"authors\":\"\",\"doi\":\"10.1016/j.cropro.2024.106875\",\"DOIUrl\":null,\"url\":null,\"abstract\":\"<div><p><em>Hirschmanniella mucronata</em> and <em>Meloidogyne graminicola</em> are the main plant-parasitic nematodes found in paddy fields in Thailand causing significant rice production yield losses. Although these nematodes are found widely in fields, an effective management method has not been documented. Here, we examined the nematicidal effects of different cavalcade leaf ages (1-, 2- and 3-month-old), used as aqueous extracts and soil amendments, on <em>H. mucronata</em> and <em>M. graminicola</em>. <em>In vitro</em> tests evidenced maximum mortality of <em>H. mucronata</em> (55.6–60.0%) in 50 mg ml<sup>−1</sup> extract from all leaf ages, while mortality (65.9%) and hatchability (67.0%) of <em>M. graminicola</em> second-stage juveniles were observed in 50 mg ml<sup>−1</sup> extract from 1-month-old leaves only. Moreover, aqueous extracts of cavalcade showed a repellent effect on both nematodes. Similarly, greenhouse experiments showed a significant reduction of <em>H. mucronata</em> population densities and reproduction of <em>M. graminicola</em> in soil amended with 0.5 and 1.0% (w/w) from each leaf age. LC-QTOF-MS analysis determined that three bioactive compounds, including quercetin, rutin, and kaempferol, are associated with the nematicidal activity of cavalcade against <em>H. mucronata</em> and <em>M. graminicola</em>. The information derived from this study indicates that the leaves of cavalcade are a source of promising phytonematicidals. This is the first study to assess and document its potential for nematicidal activity against <em>H. mucronata</em> and <em>M. graminicola</em> in Thailand. However, further research is needed to evaluate their efficacy under paddy field conditions.</p></div>\",\"PeriodicalId\":10785,\"journal\":{\"name\":\"Crop Protection\",\"volume\":null,\"pages\":null},\"PeriodicalIF\":2.5000,\"publicationDate\":\"2024-08-02\",\"publicationTypes\":\"Journal Article\",\"fieldsOfStudy\":null,\"isOpenAccess\":false,\"openAccessPdf\":\"\",\"citationCount\":\"0\",\"resultStr\":null,\"platform\":\"Semanticscholar\",\"paperid\":null,\"PeriodicalName\":\"Crop Protection\",\"FirstCategoryId\":\"97\",\"ListUrlMain\":\"https://www.sciencedirect.com/science/article/pii/S026121942400303X\",\"RegionNum\":2,\"RegionCategory\":\"农林科学\",\"ArticlePicture\":[],\"TitleCN\":null,\"AbstractTextCN\":null,\"PMCID\":null,\"EPubDate\":\"\",\"PubModel\":\"\",\"JCR\":\"Q1\",\"JCRName\":\"AGRONOMY\",\"Score\":null,\"Total\":0}","platform":"Semanticscholar","paperid":null,"PeriodicalName":"Crop Protection","FirstCategoryId":"97","ListUrlMain":"https://www.sciencedirect.com/science/article/pii/S026121942400303X","RegionNum":2,"RegionCategory":"农林科学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":null,"EPubDate":"","PubModel":"","JCR":"Q1","JCRName":"AGRONOMY","Score":null,"Total":0}
Identification of bioactive compounds in cavalcade leaves for nematicidal activity against Hirschmanniella mucronata and Meloidogyne graminicola using LC-QTOF-MS
Hirschmanniella mucronata and Meloidogyne graminicola are the main plant-parasitic nematodes found in paddy fields in Thailand causing significant rice production yield losses. Although these nematodes are found widely in fields, an effective management method has not been documented. Here, we examined the nematicidal effects of different cavalcade leaf ages (1-, 2- and 3-month-old), used as aqueous extracts and soil amendments, on H. mucronata and M. graminicola. In vitro tests evidenced maximum mortality of H. mucronata (55.6–60.0%) in 50 mg ml−1 extract from all leaf ages, while mortality (65.9%) and hatchability (67.0%) of M. graminicola second-stage juveniles were observed in 50 mg ml−1 extract from 1-month-old leaves only. Moreover, aqueous extracts of cavalcade showed a repellent effect on both nematodes. Similarly, greenhouse experiments showed a significant reduction of H. mucronata population densities and reproduction of M. graminicola in soil amended with 0.5 and 1.0% (w/w) from each leaf age. LC-QTOF-MS analysis determined that three bioactive compounds, including quercetin, rutin, and kaempferol, are associated with the nematicidal activity of cavalcade against H. mucronata and M. graminicola. The information derived from this study indicates that the leaves of cavalcade are a source of promising phytonematicidals. This is the first study to assess and document its potential for nematicidal activity against H. mucronata and M. graminicola in Thailand. However, further research is needed to evaluate their efficacy under paddy field conditions.
期刊介绍:
The Editors of Crop Protection especially welcome papers describing an interdisciplinary approach showing how different control strategies can be integrated into practical pest management programs, covering high and low input agricultural systems worldwide. Crop Protection particularly emphasizes the practical aspects of control in the field and for protected crops, and includes work which may lead in the near future to more effective control. The journal does not duplicate the many existing excellent biological science journals, which deal mainly with the more fundamental aspects of plant pathology, applied zoology and weed science. Crop Protection covers all practical aspects of pest, disease and weed control, including the following topics:
-Abiotic damage-
Agronomic control methods-
Assessment of pest and disease damage-
Molecular methods for the detection and assessment of pests and diseases-
Biological control-
Biorational pesticides-
Control of animal pests of world crops-
Control of diseases of crop plants caused by microorganisms-
Control of weeds and integrated management-
Economic considerations-
Effects of plant growth regulators-
Environmental benefits of reduced pesticide use-
Environmental effects of pesticides-
Epidemiology of pests and diseases in relation to control-
GM Crops, and genetic engineering applications-
Importance and control of postharvest crop losses-
Integrated control-
Interrelationships and compatibility among different control strategies-
Invasive species as they relate to implications for crop protection-
Pesticide application methods-
Pest management-
Phytobiomes for pest and disease control-
Resistance management-
Sampling and monitoring schemes for diseases, nematodes, pests and weeds.