{"title":"改善 MoS2/Fe3O4/GNR 纳米复合材料在癌症治疗中的光热特性。","authors":"Behdad Shariati, Mohammad Taghi Goodarzi, Alireza Jalali, Nasrin Salehi, Majid Mozaffari","doi":"10.1007/s10856-024-06819-6","DOIUrl":null,"url":null,"abstract":"<div><p>The objective of the present study was to develop a novel molybdenum disulfide/iron oxide/gold nanorods (MoS<sub>2</sub>/Fe<sub>3</sub>O<sub>4</sub>/GNR) nanocomposite (MFG) with different concentrations of AgNO<sub>3</sub> solution (MFG1, MFG2, and MFG3) for topical doxorubicin (DOX) drug delivery. Then, these nanocomposites were synthesized and characterized by Fourier transform infrared (FTIR), Transmission electron microscopy (TEM), Dynamic light scattering (DLS), and Ultraviolet-visible (UV–Vis) spectroscopies to confirm their structural and optical properties. Cytotoxicity of samples on Hela cell was determined using MTT assay. Results indicated that nanocomposites possess little cytotoxicity without NIR laser irradiation. 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引用次数: 0
摘要
本研究旨在开发一种新型二硫化钼/氧化铁/金纳米棒（MoS2/Fe3O4/GNR）纳米复合材料（MFG），并将其与不同浓度的 AgNO3 溶液（MFG1、MFG2 和 MFG3）混合，用于局部给药多柔比星（DOX）。然后，合成了这些纳米复合材料，并通过傅立叶变换红外光谱（FTIR）、透射电子显微镜（TEM）、动态光散射（DLS）和紫外可见光谱（UV-Vis）对其进行表征，以确认其结构和光学特性。使用 MTT 试验测定了样品对 Hela 细胞的细胞毒性。结果表明，在没有近红外激光照射的情况下，纳米复合材料的细胞毒性很小。此外，当该纳米复合材料中的 AgNO3 溶液浓度增加时，Hela 细胞的相对活力降低。使用近红外激光照射时，当样品浓度增加时，Hela 细胞的相对存活率降低。利用吖啶橙/碘化丙啶（PI）染色法和流式细胞术评估了这些纳米复合材料对 Hela 细胞凋亡的影响。结果表明，当纳米复合材料中的 DOX 负荷增加时，细胞活力降低。因此，这些特性使 MFG3 纳米复合材料成为光热疗法和药物负载的良好候选材料。
Improvement photothermal property of MoS2/Fe3O4/GNR nanocomposite in cancer treatment
The objective of the present study was to develop a novel molybdenum disulfide/iron oxide/gold nanorods (MoS2/Fe3O4/GNR) nanocomposite (MFG) with different concentrations of AgNO3 solution (MFG1, MFG2, and MFG3) for topical doxorubicin (DOX) drug delivery. Then, these nanocomposites were synthesized and characterized by Fourier transform infrared (FTIR), Transmission electron microscopy (TEM), Dynamic light scattering (DLS), and Ultraviolet-visible (UV–Vis) spectroscopies to confirm their structural and optical properties. Cytotoxicity of samples on Hela cell was determined using MTT assay. Results indicated that nanocomposites possess little cytotoxicity without NIR laser irradiation. Also, the relative viabilities of Hela cells decreased when the concentration of AgNO3 solution increased in this nanocomposite. Using NIR irradiation, the relative viabilities of Hela cells decreased when the concentration of samples increased. Acridine orange/propidium iodide (PI) staining, flow cytometry were recruited to evaluate the effect of these nanocomposites on apoptosis of Hela cells. Finally, results revealed when DOX loading increased in nanocomposite, then cell viability was decreased in it. Therefore, these properties make MFG3 nanocomposite a good candidate for photothermal therapy and drug loading.
期刊介绍：
The Journal of Materials Science: Materials in Medicine publishes refereed papers providing significant progress in the application of biomaterials and tissue engineering constructs as medical or dental implants, prostheses and devices. Coverage spans a wide range of topics from basic science to clinical applications, around the theme of materials in medicine and dentistry. The central element is the development of synthetic and natural materials used in orthopaedic, maxillofacial, cardiovascular, neurological, ophthalmic and dental applications. Special biomedical topics include biomaterial synthesis and characterisation, biocompatibility studies, nanomedicine, tissue engineering constructs and cell substrates, regenerative medicine, computer modelling and other advanced experimental methodologies.
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