鉴定 BiP 作为温度传感器介导温度诱导的秀丽隐杆线虫种系性别逆转。

IF 9.4 1区 生物学 Q1 BIOCHEMISTRY & MOLECULAR BIOLOGY EMBO Journal Pub Date : 2024-09-01 Epub Date: 2024-08-12 DOI:10.1038/s44318-024-00197-z
Jing Shi, Danli Sheng, Jie Guo, Fangyuan Zhou, Shaofeng Wu, Hongyun Tang
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引用次数: 0

摘要

动物的性别决定不仅由核型决定,还可以通过不明确的传导机制受温度等环境线索的调节。此外,与早期认为性别可能完全由核型或温度决定的观点不同,最近的观察表明,这些因素反而共同调控性别,提出了另一个机理之谜。在这里,我们发现某些野生分离株和突变株的秀丽隐杆线虫表现出基因型生殖系性别决定(GGSD),但具有温度覆盖机制。此外,我们还发现ER伴侣蛋白BiP能将温度信息转化为种系性别控制信号,从而使GGSD和温度依赖性种系性别决定(TGSD)共存。在分子水平上,温度升高对ER蛋白折叠的要求增加,导致BiP螯合,导致ERAD依赖性地降解卵母细胞命运驱动因子TRA-2,从而促进雄性生殖系的命运。值得注意的是,通过实验操纵 BiP 或 TRA-2 的表达可以在 GGSD 和 TGSD 之间切换。从生理学角度看,TGSD能让优雅雌雄同体在较高温度下保持育雏规模。此外,在不同的非雌雄同体线虫物种中,BiP 也能影响生殖细胞的性别决定。总之,我们的研究发现热敏性 BiP 是 TGSD 中一种保守的温度传感器,并为 GGSD 和 TGSD 之间的转变提供了机理上的见解。
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Identification of BiP as a temperature sensor mediating temperature-induced germline sex reversal in C. elegans.

Sex determination in animals is not only determined by karyotype but can also be modulated by environmental cues like temperature via unclear transduction mechanisms. Moreover, in contrast to earlier views that sex may exclusively be determined by either karyotype or temperature, recent observations suggest that these factors rather co-regulate sex, posing another mechanistic mystery. Here, we discovered that certain wild-isolated and mutant C. elegans strains displayed genotypic germline sex determination (GGSD), but with a temperature-override mechanism. Further, we found that BiP, an ER chaperone, transduces temperature information into a germline sex-governing signal, thereby enabling the coexistence of GGSD and temperature-dependent germline sex determination (TGSD). At the molecular level, increased ER protein-folding requirements upon increased temperatures lead to BiP sequestration, resulting in ERAD-dependent degradation of the oocyte fate-driving factor, TRA-2, thus promoting male germline fate. Remarkably, experimentally manipulating BiP or TRA-2 expression allows to switch between GGSD and TGSD. Physiologically, TGSD allows C. elegans hermaphrodites to maintain brood size at warmer temperatures. Moreover, BiP can also influence germline sex determination in a different, non-hermaphroditic nematode species. Collectively, our findings identify thermosensitive BiP as a conserved temperature sensor in TGSD, and provide mechanistic insights into the transition between GGSD and TGSD.

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来源期刊
EMBO Journal
EMBO Journal 生物-生化与分子生物学
CiteScore
18.90
自引率
0.90%
发文量
246
审稿时长
1.5 months
期刊介绍: The EMBO Journal has stood as EMBO's flagship publication since its inception in 1982. Renowned for its international reputation in quality and originality, the journal spans all facets of molecular biology. It serves as a platform for papers elucidating original research of broad general interest in molecular and cell biology, with a distinct focus on molecular mechanisms and physiological relevance. With a commitment to promoting articles reporting novel findings of broad biological significance, The EMBO Journal stands as a key contributor to advancing the field of molecular biology.
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