Melissa Florencio, Francisco Romulo Oilveira Magalhães, Aline Araujo Zuma, Cinara Lima Oliveira Lugathe, Dayana Rosa, Karina Riguete, Maria Cristina Machado Motta, Aparecida Alves do Nascimento, Jacenir Reis dos Santos Mallet, Georgia Correa Atella, Patrícia Fampa
{"title":"稳定蝇 Stomoxys calcitrans 唾液腺的形态分析和脂质组成强化了摄食行为的重要特征。","authors":"Melissa Florencio, Francisco Romulo Oilveira Magalhães, Aline Araujo Zuma, Cinara Lima Oliveira Lugathe, Dayana Rosa, Karina Riguete, Maria Cristina Machado Motta, Aparecida Alves do Nascimento, Jacenir Reis dos Santos Mallet, Georgia Correa Atella, Patrícia Fampa","doi":"10.1111/mve.12751","DOIUrl":null,"url":null,"abstract":"<p><i>Stomoxys calcitrans</i> L. (Diptera: Muscidae), the stable fly, is a hematophagous insect of great veterinary importance, because it is a mechanical vector of diverse pathogens in livestock. The saliva of blood-feeding insects presents important pharmacologically active molecules that impair blood clotting, promote vasodilation and modulate the host immune system response, crucial processes for successful feeding. These properties also enable pathogens' transmission. In the present work, we describe an efficient protocol to dissect <i>S. calcitrans</i> salivary glands, their morphological characteristics and lipid profile. The mean length of the tubular gland is 3.23 mm with a bulbous posterior end and a narrow anterior end. Histological analysis revealed a monolayer of large polygonal epithelial cells with voluminous nuclei and high lipid content in their cytoplasm. Ultrastructural analysis showed that the epithelium is rich in mitochondria, free ribosomes, Golgi complex cisternae, presenting a great extension of rough endoplasmic reticulum that contains an electron-dense material. Lipid analysis by thin-layer chromatography showed that neutral fatty acids and phosphatidylcholine are predominant in the fly salivary glands. Lysophosphatidylcholine, an important signalling biomolecule involved in different metabolic processes, including host's immunomodulation and pathogens proliferation and differentiation, is also present.</p>","PeriodicalId":18350,"journal":{"name":"Medical and Veterinary Entomology","volume":null,"pages":null},"PeriodicalIF":1.6000,"publicationDate":"2024-08-13","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":"0","resultStr":"{\"title\":\"Morphological analysis and lipid composition of the stable fly Stomoxys calcitrans salivary glands reinforce important features of feeding behaviour\\n Análises morfológicas e composição lipídica das glândulas salivares de Stomoxys calcitrans, a mosca-dos-estábulos, reforçam importantes características de seu comportamento alimentar\",\"authors\":\"Melissa Florencio, Francisco Romulo Oilveira Magalhães, Aline Araujo Zuma, Cinara Lima Oliveira Lugathe, Dayana Rosa, Karina Riguete, Maria Cristina Machado Motta, Aparecida Alves do Nascimento, Jacenir Reis dos Santos Mallet, Georgia Correa Atella, Patrícia Fampa\",\"doi\":\"10.1111/mve.12751\",\"DOIUrl\":null,\"url\":null,\"abstract\":\"<p><i>Stomoxys calcitrans</i> L. (Diptera: Muscidae), the stable fly, is a hematophagous insect of great veterinary importance, because it is a mechanical vector of diverse pathogens in livestock. The saliva of blood-feeding insects presents important pharmacologically active molecules that impair blood clotting, promote vasodilation and modulate the host immune system response, crucial processes for successful feeding. These properties also enable pathogens' transmission. In the present work, we describe an efficient protocol to dissect <i>S. calcitrans</i> salivary glands, their morphological characteristics and lipid profile. The mean length of the tubular gland is 3.23 mm with a bulbous posterior end and a narrow anterior end. Histological analysis revealed a monolayer of large polygonal epithelial cells with voluminous nuclei and high lipid content in their cytoplasm. Ultrastructural analysis showed that the epithelium is rich in mitochondria, free ribosomes, Golgi complex cisternae, presenting a great extension of rough endoplasmic reticulum that contains an electron-dense material. Lipid analysis by thin-layer chromatography showed that neutral fatty acids and phosphatidylcholine are predominant in the fly salivary glands. 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Morphological analysis and lipid composition of the stable fly Stomoxys calcitrans salivary glands reinforce important features of feeding behaviour
Análises morfológicas e composição lipídica das glândulas salivares de Stomoxys calcitrans, a mosca-dos-estábulos, reforçam importantes características de seu comportamento alimentar
Stomoxys calcitrans L. (Diptera: Muscidae), the stable fly, is a hematophagous insect of great veterinary importance, because it is a mechanical vector of diverse pathogens in livestock. The saliva of blood-feeding insects presents important pharmacologically active molecules that impair blood clotting, promote vasodilation and modulate the host immune system response, crucial processes for successful feeding. These properties also enable pathogens' transmission. In the present work, we describe an efficient protocol to dissect S. calcitrans salivary glands, their morphological characteristics and lipid profile. The mean length of the tubular gland is 3.23 mm with a bulbous posterior end and a narrow anterior end. Histological analysis revealed a monolayer of large polygonal epithelial cells with voluminous nuclei and high lipid content in their cytoplasm. Ultrastructural analysis showed that the epithelium is rich in mitochondria, free ribosomes, Golgi complex cisternae, presenting a great extension of rough endoplasmic reticulum that contains an electron-dense material. Lipid analysis by thin-layer chromatography showed that neutral fatty acids and phosphatidylcholine are predominant in the fly salivary glands. Lysophosphatidylcholine, an important signalling biomolecule involved in different metabolic processes, including host's immunomodulation and pathogens proliferation and differentiation, is also present.
期刊介绍:
Medical and Veterinary Entomology is the leading periodical in its field. The Journal covers the biology and control of insects, ticks, mites and other arthropods of medical and veterinary importance. The main strengths of the Journal lie in the fields of:
-epidemiology and transmission of vector-borne pathogens
changes in vector distribution that have impact on the pathogen transmission-
arthropod behaviour and ecology-
novel, field evaluated, approaches to biological and chemical control methods-
host arthropod interactions.
Please note that we do not consider submissions in forensic entomology.