稳定蝇 Stomoxys calcitrans 唾液腺的形态分析和脂质组成强化了摄食行为的重要特征。

IF 1.6 3区 农林科学 Q2 ENTOMOLOGY Medical and Veterinary Entomology Pub Date : 2024-08-13 DOI:10.1111/mve.12751
Melissa Florencio, Francisco Romulo Oilveira Magalhães, Aline Araujo Zuma, Cinara Lima Oliveira Lugathe, Dayana Rosa, Karina Riguete, Maria Cristina Machado Motta, Aparecida Alves do Nascimento, Jacenir Reis dos Santos Mallet, Georgia Correa Atella, Patrícia Fampa
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摘要

厩蝇 Stomoxys calcitrans L.(双翅目:鹟科)是一种食血昆虫,对兽医具有重要意义,因为它是家畜中各种病原体的机械载体。食血昆虫的唾液中含有重要的药理活性分子,可损害血液凝固、促进血管扩张和调节宿主免疫系统反应,这些都是成功捕食的关键过程。这些特性也使病原体得以传播。在本研究中,我们描述了一种高效的方案,用于解剖钙蟾蜍唾液腺、其形态特征和脂质概况。管状腺体的平均长度为 3.23 毫米,后端呈球状,前端狭窄。组织学分析显示,单层大的多角形上皮细胞,细胞核体积大,细胞质中脂质含量高。超微结构分析表明,上皮细胞中含有丰富的线粒体、游离核糖体和高尔基复合体空泡,粗面内质网也有很大的延伸,其中含有电子致密物质。薄层色谱法的脂质分析表明,苍蝇唾液腺中主要是中性脂肪酸和磷脂酰胆碱。溶血磷脂酰胆碱是一种重要的信号生物大分子,参与不同的代谢过程,包括宿主的免疫调节和病原体的增殖和分化。
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Morphological analysis and lipid composition of the stable fly Stomoxys calcitrans salivary glands reinforce important features of feeding behaviour Análises morfológicas e composição lipídica das glândulas salivares de Stomoxys calcitrans, a mosca-dos-estábulos, reforçam importantes características de seu comportamento alimentar

Stomoxys calcitrans L. (Diptera: Muscidae), the stable fly, is a hematophagous insect of great veterinary importance, because it is a mechanical vector of diverse pathogens in livestock. The saliva of blood-feeding insects presents important pharmacologically active molecules that impair blood clotting, promote vasodilation and modulate the host immune system response, crucial processes for successful feeding. These properties also enable pathogens' transmission. In the present work, we describe an efficient protocol to dissect S. calcitrans salivary glands, their morphological characteristics and lipid profile. The mean length of the tubular gland is 3.23 mm with a bulbous posterior end and a narrow anterior end. Histological analysis revealed a monolayer of large polygonal epithelial cells with voluminous nuclei and high lipid content in their cytoplasm. Ultrastructural analysis showed that the epithelium is rich in mitochondria, free ribosomes, Golgi complex cisternae, presenting a great extension of rough endoplasmic reticulum that contains an electron-dense material. Lipid analysis by thin-layer chromatography showed that neutral fatty acids and phosphatidylcholine are predominant in the fly salivary glands. Lysophosphatidylcholine, an important signalling biomolecule involved in different metabolic processes, including host's immunomodulation and pathogens proliferation and differentiation, is also present.

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来源期刊
Medical and Veterinary Entomology
Medical and Veterinary Entomology 农林科学-昆虫学
CiteScore
3.70
自引率
5.30%
发文量
65
审稿时长
12-24 weeks
期刊介绍: Medical and Veterinary Entomology is the leading periodical in its field. The Journal covers the biology and control of insects, ticks, mites and other arthropods of medical and veterinary importance. The main strengths of the Journal lie in the fields of: -epidemiology and transmission of vector-borne pathogens changes in vector distribution that have impact on the pathogen transmission- arthropod behaviour and ecology- novel, field evaluated, approaches to biological and chemical control methods- host arthropod interactions. Please note that we do not consider submissions in forensic entomology.
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