An automated method for thrombocyte counting in capillary microsamples

Introduction

We aimed to develop an automated, low-volume method for thrombocyte counting in capillary blood using the Sysmex predilution (PD) mode.

Methods

Microsamples were prepared by resuspension of 50 μL blood in 300 μL DCL CellPack. Thrombocyte counting was done in the impedance (PLT-I) and fluorescence (PLT-F) channels. The imprecision and bias was evaluated in >394 microsamples from adult blood. Preanalytical factors (skin-piercing, storage, and transportation in our pneumatic tube system) was assessed, and studies on pediatric microsamples were made for comparison. The improvement in analytical quality and turnaround time was examined.

Results

For PLT-F, the imprecision was 1.1%–3.7%, and the bias was 10.1% (95% CI: 8.8–11.3). After skin-piercing, the bias was 8.1% (95% CI: 5.6–10.6) and the imprecision 1.9% (95% CI: 1.3–2.5). Thrombocyte counts kept stable after 4 h at room temperature (94.8% [95% CI: 93.2–96.4]) and after pneumatic tube transportation [6.7% (95% CI: 4.8–8.6)]. The bias of the PD mode for pediatric microsamples was 13.0% (95% CI: −8.4–34.4) in the PLT-F channel. The automated method had a considerably lower imprecision than the existing manual thrombocyte counting method and reduced turnaround times.

Conclusion

The automated microsample method offers a low-volume alternative for measurement of thrombocytes. The method appears useful also in pediatric samples.