Xiaofeng Luo, Xiaojing Xu, Jiahui Xu, Xiaoting Zhao, Ranran Zhang, Yiping Shi, Mingyu Xia, Baoshan Xian, Wenguan Zhou, Chuan Zheng, Shaowei Wei, Lei Wang, Junbo Du, Weiguo Liu, Kai Shu
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Real-time quantitative RT-PCR (qRT-PCR) analysis confirmed that MT priming increases the expression levels of GA biosynthesis genes, ABA catabolism genes, and ROS biosynthesis genes while decreasing the expression of positive ABA regulatory genes. Further, measurements of ABA and GA concentrations are consistent with these trends. Following MT priming, quantification of ROS metabolism-related enzyme activities and the concentrations of H<sub>2</sub>O<sub>2</sub> and superoxide anions (O<sub>2</sub><sup>−</sup>) after MT priming were consistent with the results of transcriptome analysis and qRT-PCR. Finally, exogenous application of GA, fluridone (an ABA biosynthesis inhibitor), or H<sub>2</sub>O<sub>2</sub> partially rescued the poor germination of non-primed seeds under flooding stress. Collectively, this study uncovers the application and molecular mechanisms underlying MT priming in modulating crop seed vigor under flooding stress.</p></div>","PeriodicalId":198,"journal":{"name":"Journal of Pineal Research","volume":"76 5","pages":""},"PeriodicalIF":8.3000,"publicationDate":"2024-08-15","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":"0","resultStr":"{\"title\":\"Melatonin Priming Promotes Crop Seed Germination and Seedling Establishment Under Flooding Stress by Mediating ABA, GA, and ROS Cascades\",\"authors\":\"Xiaofeng Luo, Xiaojing Xu, Jiahui Xu, Xiaoting Zhao, Ranran Zhang, Yiping Shi, Mingyu Xia, Baoshan Xian, Wenguan Zhou, Chuan Zheng, Shaowei Wei, Lei Wang, Junbo Du, Weiguo Liu, Kai Shu\",\"doi\":\"10.1111/jpi.13004\",\"DOIUrl\":null,\"url\":null,\"abstract\":\"<div>\\n \\n <p>Both seed germination and subsequent seedling establishment are key checkpoints during the life cycle of seed plants, yet flooding stress markedly inhibits both processes, leading to economic losses from agricultural production. 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引用次数: 0
摘要
种子萌发和随后的成苗是种子植物生命周期中的关键检查点,然而洪水胁迫会明显抑制这两个过程,导致农业生产的经济损失。在这里,我们报告了褪黑激素(MT)种子萌发处理可提高大豆、小麦、玉米和紫花苜蓿等几种作物种子在洪水胁迫下的表现。转录组分析表明,褪黑激素促进种子萌发和成苗与脱落酸(ABA)、赤霉素(GA)和活性氧(ROS)生物合成和信号通路的变化有关。实时定量 RT-PCR (qRT-PCR)分析证实,MT 引物提高了 GA 生物合成基因、ABA 分解基因和 ROS 生物合成基因的表达水平,同时降低了 ABA 正调控基因的表达水平。此外,ABA 和 GA 浓度的测量结果也与这些趋势一致。在 MT 诱导后,ROS 代谢相关酶活性的定量以及 H2O2 和超氧阴离子(O2 -)的浓度与转录组分析和 qRT-PCR 的结果一致。最后,外源施用 GA、氟利酮(一种 ABA 生物合成抑制剂)或 H2O2 可部分缓解未引种种子在淹水胁迫下萌发不良的问题。总之,本研究揭示了在洪水胁迫下MT引物调节作物种子活力的应用和分子机制。
Melatonin Priming Promotes Crop Seed Germination and Seedling Establishment Under Flooding Stress by Mediating ABA, GA, and ROS Cascades
Both seed germination and subsequent seedling establishment are key checkpoints during the life cycle of seed plants, yet flooding stress markedly inhibits both processes, leading to economic losses from agricultural production. Here, we report that melatonin (MT) seed priming treatment enhances the performance of seeds from several crops, including soybean, wheat, maize, and alfalfa, under flooding stress. Transcriptome analysis revealed that MT priming promotes seed germination and seedling establishment associated with changes in abscisic acid (ABA), gibberellin (GA), and reactive oxygen species (ROS) biosynthesis and signaling pathways. Real-time quantitative RT-PCR (qRT-PCR) analysis confirmed that MT priming increases the expression levels of GA biosynthesis genes, ABA catabolism genes, and ROS biosynthesis genes while decreasing the expression of positive ABA regulatory genes. Further, measurements of ABA and GA concentrations are consistent with these trends. Following MT priming, quantification of ROS metabolism-related enzyme activities and the concentrations of H2O2 and superoxide anions (O2−) after MT priming were consistent with the results of transcriptome analysis and qRT-PCR. Finally, exogenous application of GA, fluridone (an ABA biosynthesis inhibitor), or H2O2 partially rescued the poor germination of non-primed seeds under flooding stress. Collectively, this study uncovers the application and molecular mechanisms underlying MT priming in modulating crop seed vigor under flooding stress.
期刊介绍:
The Journal of Pineal Research welcomes original scientific research on the pineal gland and melatonin in vertebrates, as well as the biological functions of melatonin in non-vertebrates, plants, and microorganisms. Criteria for publication include scientific importance, novelty, timeliness, and clarity of presentation. The journal considers experimental data that challenge current thinking and welcomes case reports contributing to understanding the pineal gland and melatonin research. Its aim is to serve researchers in all disciplines related to the pineal gland and melatonin.