Fabian Bick MSc , Claudia M. Brenis Gómez MD , Inés Lammens MD , Justine Van Moorleghem BSc , Caroline De Wolf BSc , Sam Dupont BSc , Laure Dumoutier PhD , Neal P. Smith MSc , Alexandra-Chloé Villani PhD , Robin Browaeys PhD , Jehan Alladina MD , Alexis M. Haring BSc , Benjamin D. Medoff MD , Josalyn L. Cho MD , René Bigirimana PhD , Joao Vieira MSc , Hamida Hammad PhD , Christophe Blanchetot PhD , Martijn J. Schuijs PhD , Bart N. Lambrecht MD, PhD
{"title":"白细胞介素-2 家族细胞因子 IL-9 和 IL-21 对哮喘的先天性和适应性 2 型免疫有不同的调节作用。","authors":"Fabian Bick MSc , Claudia M. Brenis Gómez MD , Inés Lammens MD , Justine Van Moorleghem BSc , Caroline De Wolf BSc , Sam Dupont BSc , Laure Dumoutier PhD , Neal P. Smith MSc , Alexandra-Chloé Villani PhD , Robin Browaeys PhD , Jehan Alladina MD , Alexis M. Haring BSc , Benjamin D. Medoff MD , Josalyn L. Cho MD , René Bigirimana PhD , Joao Vieira MSc , Hamida Hammad PhD , Christophe Blanchetot PhD , Martijn J. Schuijs PhD , Bart N. Lambrecht MD, PhD","doi":"10.1016/j.jaci.2024.07.024","DOIUrl":null,"url":null,"abstract":"<div><h3>Background</h3><div>Asthma is often accompanied by type 2 immunity rich in IL-4, IL-5, and IL-13 cytokines produced by T<sub>H</sub>2 lymphocytes or type 2 innate lymphoid cells (ILC2s). IL-2 family cytokines play a key role in the differentiation, homeostasis, and effector function of innate and adaptive lymphocytes.</div></div><div><h3>Objective</h3><div>IL-9 and IL-21 boost activation and proliferation of T<sub>H</sub>2 and ILC2s, but the relative importance and potential synergism between these γ common chain cytokines are currently unknown.</div></div><div><h3>Methods</h3><div>Using newly generated antibodies, we inhibited IL-9 and IL-21 alone or in combination in various murine models of asthma. In a translational approach using segmental allergen challenge, we recently described elevated IL-9 levels in human subjects with allergic asthma compared with nonasthmatic controls. Here, we also measured IL-21 in both groups.</div></div><div><h3>Results</h3><div>IL-9 played a central role in controlling innate IL-33–induced lung inflammation by promoting proliferation and activation of ILC2s in an IL-21–independent manner. Conversely, chronic house dust mite–induced airway inflammation, mainly driven by adaptive immunity, was solely dependent on IL-21, which controlled T<sub>H</sub>2 activation, eosinophilia, total serum IgE, and formation of tertiary lymphoid structures. In a model of innate on adaptive immunity driven by papain allergen, a clear synergy was found between both pathways, as combined anti-IL-9 or anti-IL-21 blockade was superior in reducing key asthma features. In human bronchoalveolar lavage samples we measured elevated IL-21 protein within the allergic asthmatic group compared with the allergic control group. We also found increased <em>IL21R</em> transcripts and predicted IL-21 ligand activity in various disease-associated cell subsets.</div></div><div><h3>Conclusions</h3><div>IL-9 and IL-21 play important and nonredundant roles in allergic asthma by boosting ILC2s and T<sub>H</sub>2 cells, revealing a dual IL-9 and IL-21 targeting strategy as a new and testable approach.</div></div>","PeriodicalId":14936,"journal":{"name":"Journal of Allergy and Clinical Immunology","volume":"154 5","pages":"Pages 1129-1145"},"PeriodicalIF":11.4000,"publicationDate":"2024-11-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":"0","resultStr":"{\"title\":\"IL-2 family cytokines IL-9 and IL-21 differentially regulate innate and adaptive type 2 immunity in asthma\",\"authors\":\"Fabian Bick MSc , Claudia M. Brenis Gómez MD , Inés Lammens MD , Justine Van Moorleghem BSc , Caroline De Wolf BSc , Sam Dupont BSc , Laure Dumoutier PhD , Neal P. Smith MSc , Alexandra-Chloé Villani PhD , Robin Browaeys PhD , Jehan Alladina MD , Alexis M. Haring BSc , Benjamin D. Medoff MD , Josalyn L. Cho MD , René Bigirimana PhD , Joao Vieira MSc , Hamida Hammad PhD , Christophe Blanchetot PhD , Martijn J. Schuijs PhD , Bart N. Lambrecht MD, PhD\",\"doi\":\"10.1016/j.jaci.2024.07.024\",\"DOIUrl\":null,\"url\":null,\"abstract\":\"<div><h3>Background</h3><div>Asthma is often accompanied by type 2 immunity rich in IL-4, IL-5, and IL-13 cytokines produced by T<sub>H</sub>2 lymphocytes or type 2 innate lymphoid cells (ILC2s). IL-2 family cytokines play a key role in the differentiation, homeostasis, and effector function of innate and adaptive lymphocytes.</div></div><div><h3>Objective</h3><div>IL-9 and IL-21 boost activation and proliferation of T<sub>H</sub>2 and ILC2s, but the relative importance and potential synergism between these γ common chain cytokines are currently unknown.</div></div><div><h3>Methods</h3><div>Using newly generated antibodies, we inhibited IL-9 and IL-21 alone or in combination in various murine models of asthma. In a translational approach using segmental allergen challenge, we recently described elevated IL-9 levels in human subjects with allergic asthma compared with nonasthmatic controls. Here, we also measured IL-21 in both groups.</div></div><div><h3>Results</h3><div>IL-9 played a central role in controlling innate IL-33–induced lung inflammation by promoting proliferation and activation of ILC2s in an IL-21–independent manner. Conversely, chronic house dust mite–induced airway inflammation, mainly driven by adaptive immunity, was solely dependent on IL-21, which controlled T<sub>H</sub>2 activation, eosinophilia, total serum IgE, and formation of tertiary lymphoid structures. In a model of innate on adaptive immunity driven by papain allergen, a clear synergy was found between both pathways, as combined anti-IL-9 or anti-IL-21 blockade was superior in reducing key asthma features. In human bronchoalveolar lavage samples we measured elevated IL-21 protein within the allergic asthmatic group compared with the allergic control group. We also found increased <em>IL21R</em> transcripts and predicted IL-21 ligand activity in various disease-associated cell subsets.</div></div><div><h3>Conclusions</h3><div>IL-9 and IL-21 play important and nonredundant roles in allergic asthma by boosting ILC2s and T<sub>H</sub>2 cells, revealing a dual IL-9 and IL-21 targeting strategy as a new and testable approach.</div></div>\",\"PeriodicalId\":14936,\"journal\":{\"name\":\"Journal of Allergy and Clinical Immunology\",\"volume\":\"154 5\",\"pages\":\"Pages 1129-1145\"},\"PeriodicalIF\":11.4000,\"publicationDate\":\"2024-11-01\",\"publicationTypes\":\"Journal Article\",\"fieldsOfStudy\":null,\"isOpenAccess\":false,\"openAccessPdf\":\"\",\"citationCount\":\"0\",\"resultStr\":null,\"platform\":\"Semanticscholar\",\"paperid\":null,\"PeriodicalName\":\"Journal of Allergy and Clinical Immunology\",\"FirstCategoryId\":\"3\",\"ListUrlMain\":\"https://www.sciencedirect.com/science/article/pii/S0091674924008170\",\"RegionNum\":1,\"RegionCategory\":\"医学\",\"ArticlePicture\":[],\"TitleCN\":null,\"AbstractTextCN\":null,\"PMCID\":null,\"EPubDate\":\"\",\"PubModel\":\"\",\"JCR\":\"Q1\",\"JCRName\":\"ALLERGY\",\"Score\":null,\"Total\":0}","platform":"Semanticscholar","paperid":null,"PeriodicalName":"Journal of Allergy and Clinical Immunology","FirstCategoryId":"3","ListUrlMain":"https://www.sciencedirect.com/science/article/pii/S0091674924008170","RegionNum":1,"RegionCategory":"医学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":null,"EPubDate":"","PubModel":"","JCR":"Q1","JCRName":"ALLERGY","Score":null,"Total":0}
IL-2 family cytokines IL-9 and IL-21 differentially regulate innate and adaptive type 2 immunity in asthma
Background
Asthma is often accompanied by type 2 immunity rich in IL-4, IL-5, and IL-13 cytokines produced by TH2 lymphocytes or type 2 innate lymphoid cells (ILC2s). IL-2 family cytokines play a key role in the differentiation, homeostasis, and effector function of innate and adaptive lymphocytes.
Objective
IL-9 and IL-21 boost activation and proliferation of TH2 and ILC2s, but the relative importance and potential synergism between these γ common chain cytokines are currently unknown.
Methods
Using newly generated antibodies, we inhibited IL-9 and IL-21 alone or in combination in various murine models of asthma. In a translational approach using segmental allergen challenge, we recently described elevated IL-9 levels in human subjects with allergic asthma compared with nonasthmatic controls. Here, we also measured IL-21 in both groups.
Results
IL-9 played a central role in controlling innate IL-33–induced lung inflammation by promoting proliferation and activation of ILC2s in an IL-21–independent manner. Conversely, chronic house dust mite–induced airway inflammation, mainly driven by adaptive immunity, was solely dependent on IL-21, which controlled TH2 activation, eosinophilia, total serum IgE, and formation of tertiary lymphoid structures. In a model of innate on adaptive immunity driven by papain allergen, a clear synergy was found between both pathways, as combined anti-IL-9 or anti-IL-21 blockade was superior in reducing key asthma features. In human bronchoalveolar lavage samples we measured elevated IL-21 protein within the allergic asthmatic group compared with the allergic control group. We also found increased IL21R transcripts and predicted IL-21 ligand activity in various disease-associated cell subsets.
Conclusions
IL-9 and IL-21 play important and nonredundant roles in allergic asthma by boosting ILC2s and TH2 cells, revealing a dual IL-9 and IL-21 targeting strategy as a new and testable approach.
期刊介绍：
The Journal of Allergy and Clinical Immunology is a prestigious publication that features groundbreaking research in the fields of Allergy, Asthma, and Immunology. This influential journal publishes high-impact research papers that explore various topics, including asthma, food allergy, allergic rhinitis, atopic dermatitis, primary immune deficiencies, occupational and environmental allergy, and other allergic and immunologic diseases. The articles not only report on clinical trials and mechanistic studies but also provide insights into novel therapies, underlying mechanisms, and important discoveries that contribute to our understanding of these diseases. By sharing this valuable information, the journal aims to enhance the diagnosis and management of patients in the future.
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