通过在根白质中表达 Bt 杀线虫蛋白有效控制根结线虫。

IF 17.1 1区 生物学 Q1 BIOCHEMISTRY & MOLECULAR BIOLOGY Molecular Plant Pub Date : 2024-10-07 Epub Date: 2024-08-14 DOI:10.1016/j.molp.2024.08.004
Yong Wang, Mengnan Wang, Yali Zhang, Longwei Peng, Dadong Dai, Fengjuan Zhang, Jiang Zhang
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引用次数: 0

摘要

根结线虫(RKNs)是一种感染寄主植物根部的植物害虫。苏云金芽孢杆菌(Bt)杀线虫蛋白对线虫具有毒性。然而,由于转基因植物缺乏有效的传递系统,杀线虫蛋白在植物保护中的应用受到阻碍。在这项研究中,我们发现在虫瘿和 RKN 诱导的巨细胞中积累了白质体(根质体)。通过共聚焦显微镜和免疫电镜观察,RKN 感染导致白质体降解为小囊泡状结构,负责向 RKN 运送蛋白质。我们还通过 Western 印迹分析进一步证明,白细胞中不同大小的蛋白质可被黑僵菌雌虫吸收。为了进一步探索白细胞的潜在应用,我们将 Bt 晶体蛋白 Cry5Ba2 与转运肽融合后导入烟草和番茄白细胞。由此产生的转基因植株对 RKN 具有显著的抗性。耐人寻味的是,当 Cry5Ba2 蛋白通过质体而不是细胞质传递时,RKN 雌虫会优先吸收它。后代产量的减少与杀线虫蛋白的传递效率呈正相关。总之,这项研究为了解 RKNs 的取食行为及其摄取白质体蛋白的能力提供了新的视角。此外,该研究还证明了白细胞作为杀线虫蛋白高效递送系统的潜在用途,为控制线虫提供了一种前景广阔的方法。
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Efficient control of root-knot nematodes by expressing Bt nematicidal proteins in root leucoplasts.

Root-knot nematodes (RKNs) are plant pests that infect the roots of host plants. Bacillus thuringiensis (Bt) nematicidal proteins exhibited toxicity to nematodes. However, the application of nematicidal proteins for plant protection is hampered by the lack of effective delivery systems in transgenic plants. In this study, we discovered the accumulation of leucoplasts (root plastids) in galls and RKN-induced giant cells. RKN infection causes the degradation of leucoplasts into small vesicle-like structures, which are responsible for delivering proteins to RKNs, as observed through confocal microscopy and immunoelectron microscopy. We showed that different-sized proteins from leucoplasts could be taken up by Meloidogyne incognita female. To further explore the potential applications of leucoplasts, we introduced the Bt crystal protein Cry5Ba2 into tobacco and tomato leucoplasts by fusing it with a transit peptide. The transgenic plants showed significant resistance to RKNs. Intriguingly, RKN females preferentially took up Cry5Ba2 protein when delivered through plastids rather than the cytosol. The decrease in progeny was positively correlated with the delivery efficiency of the nematicidal protein. In conclusion, this study offers new insights into the feeding behavior of RKNs and their ability to ingest leucoplast proteins, and demonstrates that root leucoplasts can be used for delivering nematicidal proteins, thereby offering a promising approach for nematode control.

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来源期刊
Molecular Plant
Molecular Plant 植物科学-生化与分子生物学
CiteScore
37.60
自引率
2.20%
发文量
1784
审稿时长
1 months
期刊介绍: Molecular Plant is dedicated to serving the plant science community by publishing novel and exciting findings with high significance in plant biology. The journal focuses broadly on cellular biology, physiology, biochemistry, molecular biology, genetics, development, plant-microbe interaction, genomics, bioinformatics, and molecular evolution. Molecular Plant publishes original research articles, reviews, Correspondence, and Spotlights on the most important developments in plant biology.
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