NADP-苹果酸酶 OsNADP-ME2 参与赤霉素信号转导调节水稻植株高度

IF 4.8 1区 农林科学 Q1 AGRONOMY Rice Pub Date : 2024-08-17 DOI:10.1186/s12284-024-00729-5
Bing Li, Xiaolong Zhou, Wei Yao, Jinjun Lin, Xiaowen Ding, Qianru Chen, Hao Huang, Wenfeng Chen, Xilai Huang, Sujun Pan, Yinghui Xiao, Jianfeng Liu, Xionglun Liu, Jinling Liu
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引用次数: 0

摘要

植物 NADP 苹果酸酶(NADP-MEs)是一类氧化脱羧酶,在生物体内介导苹果酸代谢。尽管 NADP-MEs 在调控多种生物过程中发挥着关键作用,但人们对 NADP-MEs 在植物生长发育中的作用仍然知之甚少。在此,我们研究了水稻细胞质 OsNADP-ME2 在调控植株高度中的功能。结果表明,RNAi沉默和敲除水稻中的OsNADP-ME2会导致植株矮小,与之相关的植物激素赤霉素(GA)生物合成和信号转导基因的表达受到显著抑制,但GA信号转导抑制因子SLR1的表达上调。在 RNAi 株系中,主要生物活性 GA1、GA4 和 GA7 的积累明显改变,外源 GA 处理会影响 OsNADP-ME2 RNAi 株系的矮小表型。OsNADP-ME2 的 RNAi 沉默也会导致 NADP-ME 活性的降低,进而导致丙酮酸产量的减少。因此,我们的数据揭示了植物 NADP-ME 通过调控生物活性 GAs 积累和 GA 信号转导在调节水稻株高方面的新功能,为水稻植株结构改良提供了宝贵的基因资源。
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NADP-malic Enzyme OsNADP-ME2 Modulates Plant Height Involving in Gibberellin Signaling in Rice.

Plants NADP-malic enzymes (NADP-MEs) act as a class of oxidative decarboxylase to mediate malic acid metabolism in organisms. Despite NADP-MEs have been demonstrated to play pivotal roles in regulating diverse biological processes, the role of NADP-MEs involving in plant growth and development remains rarely known. Here, we characterized the function of rice cytosolic OsNADP-ME2 in regulating plant height. The results showed that RNAi silencing and knock-out of OsNADP-ME2 in rice results in a dwarf plant structure, associating with significant expression inhibition of genes involving in phytohormone Gibberellin (GA) biosynthesis and signaling transduction, but with up-regulation for the expression of GA signaling suppressor SLR1. The accumulation of major bioactive GA1, GA4 and GA7 are evidently altered in RNAi lines, and exogenous GA treatment compromises the dwarf phenotype of OsNADP-ME2 RNAi lines. RNAi silencing of OsNADP-ME2 also causes the reduction of NADP-ME activity associating with decreased production of pyruvate. Thus, our data revealed a novel function of plant NADP-MEs in modulation of rice plant height through regulating bioactive GAs accumulation and GA signaling, and provided a valuable gene resource for rice plant architecture improvement.

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来源期刊
Rice
Rice AGRONOMY-
CiteScore
10.10
自引率
3.60%
发文量
60
审稿时长
>12 weeks
期刊介绍: Rice aims to fill a glaring void in basic and applied plant science journal publishing. This journal is the world''s only high-quality serial publication for reporting current advances in rice genetics, structural and functional genomics, comparative genomics, molecular biology and physiology, molecular breeding and comparative biology. Rice welcomes review articles and original papers in all of the aforementioned areas and serves as the primary source of newly published information for researchers and students in rice and related research.
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