从三叶一枝花壁中提取的多糖成分及其通过减轻氧化应激对链脲佐菌素诱导的糖尿病小鼠的肾保护作用。

IF 4.8 3区 化学 Q1 CHEMISTRY, MEDICINAL Natural Products and Bioprospecting Pub Date : 2024-08-19 DOI:10.1007/s13659-024-00467-7
Hai-Hui Guo, Lei Wu, Dan Mi, Xing-Yu Zhang, Fu-Mei He, Ting Lei, Fu-Sheng Wang
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引用次数: 0

摘要

滇贝母(Triplostegia glandulifera Wall,T. glandulifera)是中国云南少数民族常用来治疗肾病的一种民族药。然而,有关这种物质的肾脏保护作用的报道很少,其有效成分也不清楚。本研究采用水提取-酒精沉淀法提取了多糖组分 TGB 和 TGC,并测定了它们的分子量(Mw)和单糖组成。研究采用体外高糖诱导 HRMCs 模型和体内 STZ 诱导的糖尿病小鼠模型,考察了 TGB 和 TGC 部分对糖尿病肾病(DN)的保护作用。HPLC 分析表明,TGB 含有 D-半乳糖醛酸、D-葡萄糖、D-半乳糖和 D-阿拉伯糖,其 Mw 低于 TGC。在体外,TGB 具有浓度依赖性抗氧化活性,能有效减少 HRMC 的异常增殖,同时减轻其氧化应激。在糖尿病小鼠体内,TGB 能纠正糖脂代谢失调,减轻肾脏的氧化应激。此外,它还能改善肾功能,减少肾组织损伤。该研究表明,低 Mw 多糖(TGB)通过抗氧化应激机制具有更好的抗 DN 活性。
本文章由计算机程序翻译,如有差异,请以英文原文为准。

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Polysaccharide fraction from Triplostegia glandulifera Wall and its renoprotective effect in streptozotocin-induced diabetic mice by attenuating oxidative stress

Triplostegia glandulifera Wall (T. glandulifera) is an ethnomedicine commonly used by ethnic minorities in Yunnan, China, to treat kidney disease. However, there are few reports on the renoprotective effects of this substance, and the active ingredients remain unclear. In this study, we extracted the polysaccharide fractions TGB and TGC using the water extraction-alcohol precipitation method and determined their molecular weight (Mw) and monosaccharide composition. The study investigated the protective effects of TGB and TGC fractions against diabetic nephropathy (DN) using an in vitro high glucose-induced HRMCs model and an in vivo STZ-induced diabetic mouse model. HPLC analysis revealed that TGB contained D-galacturonic acid, D-glucose, D-galactose, and D-arabinose, and had a lower Mw than TGC. In vitro, TGB showed concentration-dependent antioxidant activity and effectively reduced abnormal proliferation and while attenuating oxidative stress in HRMCs. In mice with diabetes, TGB corrected the dysregulation of glucose-lipid metabolism and alleviated oxidative stress in the kidneys. Additionally, it improved renal function and reduced renal tissue damage. The study suggests that the low Mw polysaccharides (TGB) have better activity against DN through the antioxidative stress mechanism.

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来源期刊
Natural Products and Bioprospecting
Natural Products and Bioprospecting CHEMISTRY, MEDICINAL-
CiteScore
8.30
自引率
2.10%
发文量
39
审稿时长
13 weeks
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