中丝腺体中纤维蛋白相关基因的差异表达是由膳食差异诱导的,而膳食差异又是以品系为基础的。

IF 2.3 2区 农林科学 Q1 ENTOMOLOGY Journal of insect physiology Pub Date : 2024-08-16 DOI:10.1016/j.jinsphys.2024.104695
Tsuneyuki Tatsuke, Shuichiro Tomita
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摘要

家蚕(Bombyx mori)是鳞翅目昆虫的模式生物。它是一种寡食性昆虫,主要以桑叶为食,可用于工业生产生丝。人工饲料的开发为蚕提供了另一种营养来源;然而,一个重要的问题是,与桑叶饲养的蚕相比,人工饲料饲养的蚕产茧量较低。人工饲料和桑叶饲养的蚕在第五龄幼虫后期(蚕丝合成最活跃的时期)的丝腺差异尚不清楚。在这项研究中,我们利用三个品系,确定了人工饲料饲养的第五龄第五天蚕幼虫与桑叶饲养的第五龄第五天蚕幼虫中后期丝腺转录组的差异:大藏、Nichi01 和 J137 × C146。我们发现,中丝腺中与丝有关的基因纤维六聚氨基甲酸(fhx)、纤维蛋白轻链(fibL)和纤维蛋白重链(fibH)以及后丝腺中的 ser1 都以依赖于品系的方式差异表达。与桑叶饲养的蚕相比,人工日粮饲养的蚕,中丝腺中的fhx、fibL和fibH在Nichi01中上调,在J137 × C146中下调,而后丝腺中的ser1在J137 × C146中上调。我们的研究结果表明,蚕幼虫的食性和品系会影响其第五龄后期丝腺中产丝相关基因的表达。
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Differential expression of fibroin-related genes in middle silk glands is induced by dietary differences in a strain-dependent manner in Bombyx mori

The silkworm (Bombyx mori) is a model organism for lepidopteran insects. It is an oligophagous insect that primarily feeds on mulberry leaves and has industrial use for the production of raw silk. The development of artificial diets has provided an alternative nutrient source for silkworms; however, one significant issue is that the production of cocoons is lower in silkworms reared on artificial diets compared with those reared on mulberry leaves. The differences in the silk gland in the late-stage fifth instar silkworm larvae, when silk synthesis is most active, between those raised on artificial diets and mulberry leaves, are unknown. In this study, we identified differences in the transcriptomes of the middle and posterior silk glands of fifth instar day five silkworm larvae reared on artificial diets compared with those reared on mulberry leaves using three strains: Daizo, Nichi01, and J137 × C146. We found that the silk-related genes fibrohexamerin (fhx), fibroin-light-chain (fibL), and fibroin-heavy-chain (fibH) in the middle silk gland, and ser1 in the posterior silk gland, were differentially expressed in a strain-dependent manner. In silkworms reared on artificial diets, fhx, fibL, and fibH in the middle silk gland were upregulated in Nichi01 and downregulated in J137 × C146, whereas ser1 in the posterior silk gland was upregulated in J137 × C146 compared with silkworms reared on mulberry leaves. Our results demonstrate that the diet and strain of silkworm larvae affect the expression of genes related to silk production in their silk glands during the late fifth instar stage.

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来源期刊
Journal of insect physiology
Journal of insect physiology 生物-昆虫学
CiteScore
4.50
自引率
4.50%
发文量
77
审稿时长
57 days
期刊介绍: All aspects of insect physiology are published in this journal which will also accept papers on the physiology of other arthropods, if the referees consider the work to be of general interest. The coverage includes endocrinology (in relation to moulting, reproduction and metabolism), pheromones, neurobiology (cellular, integrative and developmental), physiological pharmacology, nutrition (food selection, digestion and absorption), homeostasis, excretion, reproduction and behaviour. Papers covering functional genomics and molecular approaches to physiological problems will also be included. Communications on structure and applied entomology can be published if the subject matter has an explicit bearing on the physiology of arthropods. Review articles and novel method papers are also welcomed.
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