{"title":"中丝腺体中纤维蛋白相关基因的差异表达是由膳食差异诱导的,而膳食差异又是以品系为基础的。","authors":"Tsuneyuki Tatsuke, Shuichiro Tomita","doi":"10.1016/j.jinsphys.2024.104695","DOIUrl":null,"url":null,"abstract":"<div><p>The silkworm (<em>Bombyx mori</em>) is a model organism for lepidopteran insects. It is an oligophagous insect that primarily feeds on mulberry leaves and has industrial use for the production of raw silk. The development of artificial diets has provided an alternative nutrient source for silkworms; however, one significant issue is that the production of cocoons is lower in silkworms reared on artificial diets compared with those reared on mulberry leaves. The differences in the silk gland in the late-stage fifth instar silkworm larvae, when silk synthesis is most active, between those raised on artificial diets and mulberry leaves, are unknown. In this study, we identified differences in the transcriptomes of the middle and posterior silk glands of fifth instar day five silkworm larvae reared on artificial diets compared with those reared on mulberry leaves using three strains: Daizo, Nichi01, and J137 × C146. We found that the silk-related genes <em>fibrohexamerin</em> (<em>fhx</em>), <em>fibroin-light-chain</em> (<em>fibL</em>), and <em>fibroin-heavy-chain</em> (<em>fibH</em>) in the middle silk gland, and <em>ser1</em> in the posterior silk gland, were differentially expressed in a strain-dependent manner. In silkworms reared on artificial diets, <em>fhx</em>, <em>fibL</em>, and <em>fibH</em> in the middle silk gland were upregulated in Nichi01 and downregulated in J137 × C146, whereas <em>ser1</em> in the posterior silk gland was upregulated in J137 × C146 compared with silkworms reared on mulberry leaves. Our results demonstrate that the diet and strain of silkworm larvae affect the expression of genes related to silk production in their silk glands during the late fifth instar stage.</p></div>","PeriodicalId":16189,"journal":{"name":"Journal of insect physiology","volume":"158 ","pages":"Article 104695"},"PeriodicalIF":2.3000,"publicationDate":"2024-08-16","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":"0","resultStr":"{\"title\":\"Differential expression of fibroin-related genes in middle silk glands is induced by dietary differences in a strain-dependent manner in Bombyx mori\",\"authors\":\"Tsuneyuki Tatsuke, Shuichiro Tomita\",\"doi\":\"10.1016/j.jinsphys.2024.104695\",\"DOIUrl\":null,\"url\":null,\"abstract\":\"<div><p>The silkworm (<em>Bombyx mori</em>) is a model organism for lepidopteran insects. It is an oligophagous insect that primarily feeds on mulberry leaves and has industrial use for the production of raw silk. The development of artificial diets has provided an alternative nutrient source for silkworms; however, one significant issue is that the production of cocoons is lower in silkworms reared on artificial diets compared with those reared on mulberry leaves. The differences in the silk gland in the late-stage fifth instar silkworm larvae, when silk synthesis is most active, between those raised on artificial diets and mulberry leaves, are unknown. In this study, we identified differences in the transcriptomes of the middle and posterior silk glands of fifth instar day five silkworm larvae reared on artificial diets compared with those reared on mulberry leaves using three strains: Daizo, Nichi01, and J137 × C146. We found that the silk-related genes <em>fibrohexamerin</em> (<em>fhx</em>), <em>fibroin-light-chain</em> (<em>fibL</em>), and <em>fibroin-heavy-chain</em> (<em>fibH</em>) in the middle silk gland, and <em>ser1</em> in the posterior silk gland, were differentially expressed in a strain-dependent manner. In silkworms reared on artificial diets, <em>fhx</em>, <em>fibL</em>, and <em>fibH</em> in the middle silk gland were upregulated in Nichi01 and downregulated in J137 × C146, whereas <em>ser1</em> in the posterior silk gland was upregulated in J137 × C146 compared with silkworms reared on mulberry leaves. Our results demonstrate that the diet and strain of silkworm larvae affect the expression of genes related to silk production in their silk glands during the late fifth instar stage.</p></div>\",\"PeriodicalId\":16189,\"journal\":{\"name\":\"Journal of insect physiology\",\"volume\":\"158 \",\"pages\":\"Article 104695\"},\"PeriodicalIF\":2.3000,\"publicationDate\":\"2024-08-16\",\"publicationTypes\":\"Journal Article\",\"fieldsOfStudy\":null,\"isOpenAccess\":false,\"openAccessPdf\":\"\",\"citationCount\":\"0\",\"resultStr\":null,\"platform\":\"Semanticscholar\",\"paperid\":null,\"PeriodicalName\":\"Journal of insect physiology\",\"FirstCategoryId\":\"97\",\"ListUrlMain\":\"https://www.sciencedirect.com/science/article/pii/S0022191024000830\",\"RegionNum\":2,\"RegionCategory\":\"农林科学\",\"ArticlePicture\":[],\"TitleCN\":null,\"AbstractTextCN\":null,\"PMCID\":null,\"EPubDate\":\"\",\"PubModel\":\"\",\"JCR\":\"Q1\",\"JCRName\":\"ENTOMOLOGY\",\"Score\":null,\"Total\":0}","platform":"Semanticscholar","paperid":null,"PeriodicalName":"Journal of insect physiology","FirstCategoryId":"97","ListUrlMain":"https://www.sciencedirect.com/science/article/pii/S0022191024000830","RegionNum":2,"RegionCategory":"农林科学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":null,"EPubDate":"","PubModel":"","JCR":"Q1","JCRName":"ENTOMOLOGY","Score":null,"Total":0}
Differential expression of fibroin-related genes in middle silk glands is induced by dietary differences in a strain-dependent manner in Bombyx mori
The silkworm (Bombyx mori) is a model organism for lepidopteran insects. It is an oligophagous insect that primarily feeds on mulberry leaves and has industrial use for the production of raw silk. The development of artificial diets has provided an alternative nutrient source for silkworms; however, one significant issue is that the production of cocoons is lower in silkworms reared on artificial diets compared with those reared on mulberry leaves. The differences in the silk gland in the late-stage fifth instar silkworm larvae, when silk synthesis is most active, between those raised on artificial diets and mulberry leaves, are unknown. In this study, we identified differences in the transcriptomes of the middle and posterior silk glands of fifth instar day five silkworm larvae reared on artificial diets compared with those reared on mulberry leaves using three strains: Daizo, Nichi01, and J137 × C146. We found that the silk-related genes fibrohexamerin (fhx), fibroin-light-chain (fibL), and fibroin-heavy-chain (fibH) in the middle silk gland, and ser1 in the posterior silk gland, were differentially expressed in a strain-dependent manner. In silkworms reared on artificial diets, fhx, fibL, and fibH in the middle silk gland were upregulated in Nichi01 and downregulated in J137 × C146, whereas ser1 in the posterior silk gland was upregulated in J137 × C146 compared with silkworms reared on mulberry leaves. Our results demonstrate that the diet and strain of silkworm larvae affect the expression of genes related to silk production in their silk glands during the late fifth instar stage.
期刊介绍:
All aspects of insect physiology are published in this journal which will also accept papers on the physiology of other arthropods, if the referees consider the work to be of general interest. The coverage includes endocrinology (in relation to moulting, reproduction and metabolism), pheromones, neurobiology (cellular, integrative and developmental), physiological pharmacology, nutrition (food selection, digestion and absorption), homeostasis, excretion, reproduction and behaviour. Papers covering functional genomics and molecular approaches to physiological problems will also be included. Communications on structure and applied entomology can be published if the subject matter has an explicit bearing on the physiology of arthropods. Review articles and novel method papers are also welcomed.