将软红冬小麦栽培品种 AGS2000 的茎锈病抗性精细映射到 6D 染色体上的 NLR 基因簇。

IF 4.4 1区 农林科学 Q1 AGRONOMY Theoretical and Applied Genetics Pub Date : 2024-08-19 DOI:10.1007/s00122-024-04702-0
L Rivera-Burgos, C VanGessel, M Guedira, J Smith, D Marshall, Y Jin, M Rouse, G Brown-Guedira
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引用次数: 0

摘要

Puccinia graminis f. sp. tritici (Pgt) Ug99-emerging剧毒品系对全球小麦生产构成了重大挑战。为了满足当前和未来的需求,必须找到新的抗性来源。需要鉴定能够追踪抗性基因的标记,以部署抗击高致病性病原物品系的战略。对一个 DH 群体的田间评估发现了一个抗茎锈病(Sr)的 QTL,即来自育种品系 MD01W28-08-11 的 QSr.nc-6D,该 QTL 位于染色体臂 6DS 的远端区域,在该区域已鉴定出 Sr 抗性基因 Sr42、SrCad 和 SrTmp。在 AGS2000 × LA95135 杂交的一个 DH 群体和一个 RIL 群体中发现了幼苗抗 Pgt 杂交种 TTKSK 的基因座。抗性栽培品种 AGS2000 在 MD01W28-08-11 的血统中,我们的研究结果表明它是该育种品系的 Sr 抗性来源。我们利用已发表的标记和外显子组捕获数据,丰富了 QSr.nc-6D 侧翼 10 Mb 区域的标记密度。我们在杂合子近交系中进行了精细作图,发现了三个与抗性共分离的标记,并将 QSr.nc-6D 限定在 1.3 Mb 的区域内。我们进一步利用来自其他基因组组装的信息,确定了 6DS 中携带 NLR 基因簇的共线区域。与我们的共分离 SNP 相对应的 KASP 检测方法的评估表明,它们可用于在育种计划中跟踪这种 Sr 抗性。然而,我们的研究结果也强调了在缺乏抗性基因型基因组序列的情况下,在这种复杂区域中鉴定抗性基因所面临的挑战。
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Fine mapping of stem rust resistance derived from soft red winter wheat cultivar AGS2000 to an NLR gene cluster on chromosome 6D.

The Puccinia graminis f. sp. tritici (Pgt) Ug99-emerging virulent races present a major challenge to global wheat production. To meet present and future needs, new sources of resistance must be found. Identification of markers that allow tracking of resistance genes is needed for deployment strategies to combat highly virulent pathogen races. Field evaluation of a DH population located a QTL for stem rust (Sr) resistance, QSr.nc-6D from the breeding line MD01W28-08-11 to the distal region of chromosome arm 6DS where Sr resistance genes Sr42, SrCad, and SrTmp have been identified. A locus for seedling resistance to Pgt race TTKSK was identified in a DH population and an RIL population derived from the cross AGS2000 × LA95135. The resistant cultivar AGS2000 is in the pedigree of MD01W28-08-11 and our results suggest that it is the source of Sr resistance in this breeding line. We exploited published markers and exome capture data to enrich marker density in a 10 Mb region flanking QSr.nc-6D. Our fine mapping in heterozygous inbred families identified three markers co-segregating with resistance and delimited QSr.nc-6D to a 1.3 Mb region. We further exploited information from other genome assemblies and identified collinear regions of 6DS harboring clusters of NLR genes. Evaluation of KASP assays corresponding to our co-segregating SNP suggests that they can be used to track this Sr resistance in breeding programs. However, our results also underscore the challenges posed in identifying genes underlying resistance in such complex regions in the absence of genome sequence from the resistant genotypes.

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来源期刊
CiteScore
9.60
自引率
7.40%
发文量
241
审稿时长
2.3 months
期刊介绍: Theoretical and Applied Genetics publishes original research and review articles in all key areas of modern plant genetics, plant genomics and plant biotechnology. All work needs to have a clear genetic component and significant impact on plant breeding. Theoretical considerations are only accepted in combination with new experimental data and/or if they indicate a relevant application in plant genetics or breeding. Emphasizing the practical, the journal focuses on research into leading crop plants and articles presenting innovative approaches.
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