利用克拉克电极装置作为小型昆虫的呼吸计:在蚂蚁身上进行的令人信服的测试,可以检测到不连续的呼吸。

IF 2.3 2区 农林科学 Q1 ENTOMOLOGY Journal of insect physiology Pub Date : 2024-08-17 DOI:10.1016/j.jinsphys.2024.104698
{"title":"利用克拉克电极装置作为小型昆虫的呼吸计:在蚂蚁身上进行的令人信服的测试,可以检测到不连续的呼吸。","authors":"","doi":"10.1016/j.jinsphys.2024.104698","DOIUrl":null,"url":null,"abstract":"<div><p>Respirometry provides a direct measure of an organism’s O<sub>2</sub> consumption rate (VO<sub>2</sub>), which is a significant component of its metabolic rate (energy expenditure). Amongst ants, variations in lifespan between different social castes (such as workers and queens) can be substantial, varying depending on the species. As metabolic rate is higher in short-living species, we aimed to determine how VO<sub>2</sub> and longevity may have coevolved within ant casts. Measuring VO<sub>2</sub> in such tiny animal models can be challenging, and as a first methodological step, we validate the use of a Clark electrode, initially designed for measuring mitochondrial respiration control pathways, for assessing VO<sub>2</sub> in ants within a sealed chamber. This was done by comparing it with stop-flow VO<sub>2</sub> and CO<sub>2</sub> production, using a traditional indirect calorimetry device. The global aim is to provide a reliable protocol to conduct accurate comparisons of metabolic rates within and among ant species. As expected, using the Clark electrode entails high time resolution and revealed that queens and workers exhibited discontinuous gas exchange, with episodes of apnea lasting up to 20 min.</p></div>","PeriodicalId":16189,"journal":{"name":"Journal of insect physiology","volume":null,"pages":null},"PeriodicalIF":2.3000,"publicationDate":"2024-08-17","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":"0","resultStr":"{\"title\":\"Utilization of a Clark electrode device as a respirometer for small insects: A convincing test on ants allowing to detect discontinuous gas exchange\",\"authors\":\"\",\"doi\":\"10.1016/j.jinsphys.2024.104698\",\"DOIUrl\":null,\"url\":null,\"abstract\":\"<div><p>Respirometry provides a direct measure of an organism’s O<sub>2</sub> consumption rate (VO<sub>2</sub>), which is a significant component of its metabolic rate (energy expenditure). Amongst ants, variations in lifespan between different social castes (such as workers and queens) can be substantial, varying depending on the species. As metabolic rate is higher in short-living species, we aimed to determine how VO<sub>2</sub> and longevity may have coevolved within ant casts. Measuring VO<sub>2</sub> in such tiny animal models can be challenging, and as a first methodological step, we validate the use of a Clark electrode, initially designed for measuring mitochondrial respiration control pathways, for assessing VO<sub>2</sub> in ants within a sealed chamber. This was done by comparing it with stop-flow VO<sub>2</sub> and CO<sub>2</sub> production, using a traditional indirect calorimetry device. The global aim is to provide a reliable protocol to conduct accurate comparisons of metabolic rates within and among ant species. As expected, using the Clark electrode entails high time resolution and revealed that queens and workers exhibited discontinuous gas exchange, with episodes of apnea lasting up to 20 min.</p></div>\",\"PeriodicalId\":16189,\"journal\":{\"name\":\"Journal of insect physiology\",\"volume\":null,\"pages\":null},\"PeriodicalIF\":2.3000,\"publicationDate\":\"2024-08-17\",\"publicationTypes\":\"Journal Article\",\"fieldsOfStudy\":null,\"isOpenAccess\":false,\"openAccessPdf\":\"\",\"citationCount\":\"0\",\"resultStr\":null,\"platform\":\"Semanticscholar\",\"paperid\":null,\"PeriodicalName\":\"Journal of insect physiology\",\"FirstCategoryId\":\"97\",\"ListUrlMain\":\"https://www.sciencedirect.com/science/article/pii/S0022191024000866\",\"RegionNum\":2,\"RegionCategory\":\"农林科学\",\"ArticlePicture\":[],\"TitleCN\":null,\"AbstractTextCN\":null,\"PMCID\":null,\"EPubDate\":\"\",\"PubModel\":\"\",\"JCR\":\"Q1\",\"JCRName\":\"ENTOMOLOGY\",\"Score\":null,\"Total\":0}","platform":"Semanticscholar","paperid":null,"PeriodicalName":"Journal of insect physiology","FirstCategoryId":"97","ListUrlMain":"https://www.sciencedirect.com/science/article/pii/S0022191024000866","RegionNum":2,"RegionCategory":"农林科学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":null,"EPubDate":"","PubModel":"","JCR":"Q1","JCRName":"ENTOMOLOGY","Score":null,"Total":0}
引用次数: 0

摘要

呼吸测定法可直接测量生物体的氧气消耗率(VO2),这是生物体代谢率(能量消耗)的重要组成部分。在蚂蚁中,不同社会等级(如工蚁和蚁后)之间的寿命差异很大,因物种而异。由于短寿命物种的新陈代谢率较高,我们的目标是确定 VO2 和寿命在蚂蚁种群中是如何共同进化的。在如此微小的动物模型中测量 VO2 可能具有挑战性,作为方法论的第一步,我们验证了使用克拉克电极(最初设计用于测量线粒体呼吸控制途径)评估密封舱中蚂蚁的 VO2。为此,我们使用传统的间接量热装置,将其与停止流动的 VO2 和二氧化碳产生量进行了比较。总体目标是提供一种可靠的方案,对蚂蚁物种内部和物种之间的代谢率进行准确的比较。不出所料,使用克拉克电极可以获得很高的时间分辨率,并发现蚁后和工蚁表现出不连续的呼吸,呼吸暂停可持续长达 20 分钟。
本文章由计算机程序翻译,如有差异,请以英文原文为准。

摘要图片

查看原文
分享 分享
微信好友 朋友圈 QQ好友 复制链接
本刊更多论文
Utilization of a Clark electrode device as a respirometer for small insects: A convincing test on ants allowing to detect discontinuous gas exchange

Respirometry provides a direct measure of an organism’s O2 consumption rate (VO2), which is a significant component of its metabolic rate (energy expenditure). Amongst ants, variations in lifespan between different social castes (such as workers and queens) can be substantial, varying depending on the species. As metabolic rate is higher in short-living species, we aimed to determine how VO2 and longevity may have coevolved within ant casts. Measuring VO2 in such tiny animal models can be challenging, and as a first methodological step, we validate the use of a Clark electrode, initially designed for measuring mitochondrial respiration control pathways, for assessing VO2 in ants within a sealed chamber. This was done by comparing it with stop-flow VO2 and CO2 production, using a traditional indirect calorimetry device. The global aim is to provide a reliable protocol to conduct accurate comparisons of metabolic rates within and among ant species. As expected, using the Clark electrode entails high time resolution and revealed that queens and workers exhibited discontinuous gas exchange, with episodes of apnea lasting up to 20 min.

求助全文
通过发布文献求助,成功后即可免费获取论文全文。 去求助
来源期刊
Journal of insect physiology
Journal of insect physiology 生物-昆虫学
CiteScore
4.50
自引率
4.50%
发文量
77
审稿时长
57 days
期刊介绍: All aspects of insect physiology are published in this journal which will also accept papers on the physiology of other arthropods, if the referees consider the work to be of general interest. The coverage includes endocrinology (in relation to moulting, reproduction and metabolism), pheromones, neurobiology (cellular, integrative and developmental), physiological pharmacology, nutrition (food selection, digestion and absorption), homeostasis, excretion, reproduction and behaviour. Papers covering functional genomics and molecular approaches to physiological problems will also be included. Communications on structure and applied entomology can be published if the subject matter has an explicit bearing on the physiology of arthropods. Review articles and novel method papers are also welcomed.
期刊最新文献
Central role of squid gene during oocyte development in the Hemiptera Rhodnius prolixus. Aversive sexual learning in the kissing bug Rhodnius prolixus: Modulation of different sexual responses in males and females The effects of diet and semiochemical exposure on male Bactrocera dorsalis (Hendel) (Diptera: Tephritidae) metabolic rate at a range of temperatures Attractiveness versus stickiness: Behavioural preferences of Drosophila melanogaster with competing visual stimuli Gene regulation by mating depends on time, diet, and body region in female Aedes aegypti
×
引用
GB/T 7714-2015
复制
MLA
复制
APA
复制
导出至
BibTeX EndNote RefMan NoteFirst NoteExpress
×
×
提示
您的信息不完整,为了账户安全,请先补充。
现在去补充
×
提示
您因"违规操作"
具体请查看互助需知
我知道了
×
提示
现在去查看 取消
×
提示
确定
0
微信
客服QQ
Book学术公众号 扫码关注我们
反馈
×
意见反馈
请填写您的意见或建议
请填写您的手机或邮箱
已复制链接
已复制链接
快去分享给好友吧!
我知道了
×
扫码分享
扫码分享
Book学术官方微信
Book学术文献互助
Book学术文献互助群
群 号:481959085
Book学术
文献互助 智能选刊 最新文献 互助须知 联系我们:info@booksci.cn
Book学术提供免费学术资源搜索服务,方便国内外学者检索中英文文献。致力于提供最便捷和优质的服务体验。
Copyright © 2023 Book学术 All rights reserved.
ghs 京公网安备 11010802042870号 京ICP备2023020795号-1