肿瘤免疫微环境背景下犬骨肉瘤中巨噬细胞集落刺激因子(M-CSF)和白细胞介素-34(IL-34)的表达。

IF 2.3 2区 农林科学 Q1 VETERINARY SCIENCES Veterinary and comparative oncology Pub Date : 2024-12-01 Epub Date: 2024-08-20 DOI:10.1111/vco.12993
Christopher J Robinson, Cambri E Moeller, Cally N Quick, Christine E Goodermuth, Mariano Carossino, Sita S Withers
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引用次数: 0

摘要

犬骨肉瘤(OSA)是一种已被证明能调节宿主免疫系统的恶性肿瘤。巨噬细胞集落刺激因子(M-CSF;CSF1)和白细胞介素-34(IL-34;IL34)都是集落刺激因子 1 受体(CSF-1R)的配体,可能在包括 OSA 在内的多种人类癌症的发病机制中发挥作用。本研究旨在:(1) 评估犬 OSA 细胞系和组织样本中 M-CSF 和 IL-34 的表达;(2) 确定犬 OSA 组织中 M-CSF 和 IL-34 的表达与免疫细胞浸润之间的相关性。用对照培养基、TNFα(10 毫微克/毫升)或 IL-1β(10 毫微克/毫升)处理四种犬 OSA 细胞系和犬成骨细胞,并用 RT-qPCR 和 ELISA 进行分析。在所有细胞系中都能检测到 IL-34 和 M-CSF mRNA 和蛋白质,但只有在暴露于 TNFα 或 IL-1β 后才能持续观察到转录表达的上调。OSA 细胞系中 CSF1 和 IL34 mRNA 的基线表达量等于或高于犬成骨细胞。所有 10 个 OSA 组织样本都不同程度地表达了 IL34 和 CSF1 转录本。此外,CSF1和IL34的表达均与M1巨噬细胞系相关转录本(CD80和IL15RA)呈中度至高度相关。CSF1 与 CD163 呈中度相关,但 IL34 与 M2 巨噬细胞相关转录本(CD163 和 CCL24)均无相关性。总之,IL-34 和 M-CSF 在犬 OSA 细胞系和组织中均有表达,其表达与多种免疫相关转录本呈正相关。
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Macrophage Colony Stimulating Factor (M-CSF) and Interleukin-34 (IL-34) Expression in Canine Osteosarcoma in the Context of the Tumour Immune Microenvironment.

Canine osteosarcoma (OSA) is a malignancy that has been shown to modulate the host immune system. Macrophage colony-stimulating factor (M-CSF; CSF1) and interleukin-34 (IL-34; IL34) are both ligands of colony stimulating factor 1 receptor (CSF-1R), and may play a role in the pathogenesis of a variety of human cancers, including OSA. This study aimed to, (1) assess M-CSF and IL-34 expression in canine OSA cell lines and tissue samples, and (2) determine any correlations between M-CSF and IL-34 expression and immune cell infiltrates within canine OSA tissues. Four canine OSA cell lines and canine osteoblasts were treated with control media, TNFα (10 ng/mL) or IL-1β (10 ng/mL) and analysed with RT-qPCR and ELISA. IL-34 and M-CSF mRNA and protein were detectable in all cell lines, however upregulation following TNFα or IL-1β exposure was only consistently observed for transcript expression. Baseline expression of CSF1 and IL34 mRNA in OSA cell lines was equal to or higher than that of canine osteoblasts. All 10 OSA tissue samples expressed IL34 and CSF1 transcripts to varying degrees. Furthermore, CSF1 and IL34 expression both showed a moderate to high degree of correlation with M1 macrophage lineage-associated transcripts (CD80 and IL15RA). There was a moderate degree of correlation between CSF1 and CD163, but no correlation between IL34 and either M2 macrophage-associated transcripts (CD163 and CCL24). In summary, IL-34 and M-CSF are expressed in canine OSA cell lines and tissues, and expression positively correlates with a wide range of immune-related transcripts.

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来源期刊
Veterinary and comparative oncology
Veterinary and comparative oncology 农林科学-兽医学
CiteScore
4.80
自引率
9.50%
发文量
75
审稿时长
>24 weeks
期刊介绍: Veterinary and Comparative Oncology (VCO) is an international, peer-reviewed journal integrating clinical and scientific information from a variety of related disciplines and from worldwide sources for all veterinary oncologists and cancer researchers concerned with aetiology, diagnosis and clinical course of cancer in domestic animals and its prevention. With the ultimate aim of diminishing suffering from cancer, the journal supports the transfer of knowledge in all aspects of veterinary oncology, from the application of new laboratory technology to cancer prevention, early detection, diagnosis and therapy. In addition to original articles, the journal publishes solicited editorials, review articles, commentary, correspondence and abstracts from the published literature. Accordingly, studies describing laboratory work performed exclusively in purpose-bred domestic animals (e.g. dogs, cats, horses) will not be considered.
期刊最新文献
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