Mohammed Alkhadhrh, Reem Issa, Lidia Kamal Al-Halaseh, Lilian Alnsour, Ahmad Alsarayreh, Yaseen Al Qaisi, Sina M Matalqah, Safwan Aladwan
{"title":"研究类胡萝卜素强化的Helix aspersa粘液对氧化应激、胶原酶和酪氨酸酶活性的抑制作用。","authors":"Mohammed Alkhadhrh, Reem Issa, Lidia Kamal Al-Halaseh, Lilian Alnsour, Ahmad Alsarayreh, Yaseen Al Qaisi, Sina M Matalqah, Safwan Aladwan","doi":"10.1111/jocd.16455","DOIUrl":null,"url":null,"abstract":"<p><strong>Background: </strong>The investigations of snail slime and its possible biological activities have been performed recently.</p><p><strong>Aim: </strong>This study aimed to evaluate the properties of Helix aspersa snail slime (HAS), and carotenoids-fortified slime (HASC).</p><p><strong>Methods: </strong>Snails were fed with carrots for 10 days. Slime was collected and extracted using water. Saponins identification, antioxidant, collagenase, and tyrosinase enzyme inhibition activities, carotenoids, total phenols, and flavonoids content, were determined. UHPLC-MS/MS analysis was performed for phytochemical characterization.</p><p><strong>Results: </strong>Saponins were detected in the HAS extract only. However, HASC was shown to contain a higher carotenoid content than HAS (29.51 ± 0.4 vs. 18.11 ± 0.2 μg/g). similarly, total phenolic and flavonoid content were higher for the the HASC extract compared to the HAS (182.3 ± 5.2 vs. 150.28 ± 3.3 mg/g equivalent to gallic acid), and (77.62 ± 1.2 vs. 14.19 ± 0.9 mg/g equivalent to quercetin). As expected, the HASC extract exhibited higher antioxidant activity compared to the HAS, using DPPH and the ABTS assays (IC<sub>50</sub> = 7.75 ± 0.14 vs. 20.1 ± 0.4 μg/mL), and (IC<sub>50</sub> = 7.6 ± 0.26 vs. 19.57 ± 1.4 μg/mL). UHPLC-MS/MS analysis revealed the presence of several phytocomponents of which, hexadecanoic acid and ascorbic acid, were observed in the HASC extract. Furthermore, HASC extract exhibited superior inhibitory activity compared to HAS against collagenase and tyrosinase enzymes (IC<sub>50</sub> = 8.4 ± 1.19 vs. 15.3 ± 1.12 μg/mL) and (IC<sub>50</sub> = 30.1 ± 0.91 vs. 35 ± 1.3 μg/mL).</p><p><strong>Conclusion: </strong>These findings highlight the potential of HASC as a valuable ingredient in various pharmaceutical applications, due to their content of various phenolic, antioxidants, carotenoids, hexadecanoic, and ascorbic acids. The latter is well known for its great cosmeceutical applications used for slowing the process of skin aging.</p>","PeriodicalId":15546,"journal":{"name":"Journal of Cosmetic Dermatology","volume":null,"pages":null},"PeriodicalIF":2.3000,"publicationDate":"2024-08-21","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":"0","resultStr":"{\"title\":\"Investigating the inhibitory effects of carotenoids-fortified Helix aspersa slime on oxidative stress, collagenase and tyrosinase enzyme activities.\",\"authors\":\"Mohammed Alkhadhrh, Reem Issa, Lidia Kamal Al-Halaseh, Lilian Alnsour, Ahmad Alsarayreh, Yaseen Al Qaisi, Sina M Matalqah, Safwan Aladwan\",\"doi\":\"10.1111/jocd.16455\",\"DOIUrl\":null,\"url\":null,\"abstract\":\"<p><strong>Background: </strong>The investigations of snail slime and its possible biological activities have been performed recently.</p><p><strong>Aim: </strong>This study aimed to evaluate the properties of Helix aspersa snail slime (HAS), and carotenoids-fortified slime (HASC).</p><p><strong>Methods: </strong>Snails were fed with carrots for 10 days. Slime was collected and extracted using water. Saponins identification, antioxidant, collagenase, and tyrosinase enzyme inhibition activities, carotenoids, total phenols, and flavonoids content, were determined. UHPLC-MS/MS analysis was performed for phytochemical characterization.</p><p><strong>Results: </strong>Saponins were detected in the HAS extract only. However, HASC was shown to contain a higher carotenoid content than HAS (29.51 ± 0.4 vs. 18.11 ± 0.2 μg/g). similarly, total phenolic and flavonoid content were higher for the the HASC extract compared to the HAS (182.3 ± 5.2 vs. 150.28 ± 3.3 mg/g equivalent to gallic acid), and (77.62 ± 1.2 vs. 14.19 ± 0.9 mg/g equivalent to quercetin). As expected, the HASC extract exhibited higher antioxidant activity compared to the HAS, using DPPH and the ABTS assays (IC<sub>50</sub> = 7.75 ± 0.14 vs. 20.1 ± 0.4 μg/mL), and (IC<sub>50</sub> = 7.6 ± 0.26 vs. 19.57 ± 1.4 μg/mL). UHPLC-MS/MS analysis revealed the presence of several phytocomponents of which, hexadecanoic acid and ascorbic acid, were observed in the HASC extract. Furthermore, HASC extract exhibited superior inhibitory activity compared to HAS against collagenase and tyrosinase enzymes (IC<sub>50</sub> = 8.4 ± 1.19 vs. 15.3 ± 1.12 μg/mL) and (IC<sub>50</sub> = 30.1 ± 0.91 vs. 35 ± 1.3 μg/mL).</p><p><strong>Conclusion: </strong>These findings highlight the potential of HASC as a valuable ingredient in various pharmaceutical applications, due to their content of various phenolic, antioxidants, carotenoids, hexadecanoic, and ascorbic acids. 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引用次数: 0
摘要
背景:最近对蜗牛粘液及其可能的生物活性进行了研究:目的:本研究旨在评估 Helix aspersa 蜗牛粘液(HAS)和类胡萝卜素强化粘液(HASC)的特性:方法:用胡萝卜喂养蜗牛10天。方法:用胡萝卜喂养蜗牛 10 天,收集粘液并用水提取。测定皂甙鉴定、抗氧化剂、胶原酶和酪氨酸酶抑制活性、类胡萝卜素、总酚和类黄酮含量。对植物化学特征进行了超高效液相色谱-质谱/质谱分析:结果:仅在 HAS 提取物中检测到皂苷。同样,与 HAS 相比,HASC 提取物的总酚和类黄酮含量更高(182.3 ± 5.2 vs. 150.28 ± 3.3 mg/g,相当于没食子酸)和(77.62 ± 1.2 vs. 14.19 ± 0.9 mg/g,相当于槲皮素)。正如预期的那样,与 HAS 相比,HASC 提取物在 DPPH 和 ABTS 试验中表现出更高的抗氧化活性(IC50 = 7.75 ± 0.14 vs. 20.1 ± 0.4 μg/mL)和(IC50 = 7.6 ± 0.26 vs. 19.57 ± 1.4 μg/mL)。超高效液相色谱-质谱-质谱分析表明,HASC 提取物中含有多种植物成分,其中包括十六烷酸和抗坏血酸。此外,HASC 提取物对胶原酶和酪氨酸酶(IC50 = 8.4 ± 1.19 vs. 15.3 ± 1.12 μg/mL)和(IC50 = 30.1 ± 0.91 vs. 35 ± 1.3 μg/mL)的抑制活性优于 HAS:这些发现凸显了 HASC 作为各种药物应用中的重要成分的潜力,因为它们含有各种酚类、抗氧化剂、类胡萝卜素、十六烷酸和抗坏血酸。其中,抗坏血酸因其在减缓皮肤衰老方面的巨大药用价值而闻名。
Investigating the inhibitory effects of carotenoids-fortified Helix aspersa slime on oxidative stress, collagenase and tyrosinase enzyme activities.
Background: The investigations of snail slime and its possible biological activities have been performed recently.
Aim: This study aimed to evaluate the properties of Helix aspersa snail slime (HAS), and carotenoids-fortified slime (HASC).
Methods: Snails were fed with carrots for 10 days. Slime was collected and extracted using water. Saponins identification, antioxidant, collagenase, and tyrosinase enzyme inhibition activities, carotenoids, total phenols, and flavonoids content, were determined. UHPLC-MS/MS analysis was performed for phytochemical characterization.
Results: Saponins were detected in the HAS extract only. However, HASC was shown to contain a higher carotenoid content than HAS (29.51 ± 0.4 vs. 18.11 ± 0.2 μg/g). similarly, total phenolic and flavonoid content were higher for the the HASC extract compared to the HAS (182.3 ± 5.2 vs. 150.28 ± 3.3 mg/g equivalent to gallic acid), and (77.62 ± 1.2 vs. 14.19 ± 0.9 mg/g equivalent to quercetin). As expected, the HASC extract exhibited higher antioxidant activity compared to the HAS, using DPPH and the ABTS assays (IC50 = 7.75 ± 0.14 vs. 20.1 ± 0.4 μg/mL), and (IC50 = 7.6 ± 0.26 vs. 19.57 ± 1.4 μg/mL). UHPLC-MS/MS analysis revealed the presence of several phytocomponents of which, hexadecanoic acid and ascorbic acid, were observed in the HASC extract. Furthermore, HASC extract exhibited superior inhibitory activity compared to HAS against collagenase and tyrosinase enzymes (IC50 = 8.4 ± 1.19 vs. 15.3 ± 1.12 μg/mL) and (IC50 = 30.1 ± 0.91 vs. 35 ± 1.3 μg/mL).
Conclusion: These findings highlight the potential of HASC as a valuable ingredient in various pharmaceutical applications, due to their content of various phenolic, antioxidants, carotenoids, hexadecanoic, and ascorbic acids. The latter is well known for its great cosmeceutical applications used for slowing the process of skin aging.
期刊介绍:
The Journal of Cosmetic Dermatology publishes high quality, peer-reviewed articles on all aspects of cosmetic dermatology with the aim to foster the highest standards of patient care in cosmetic dermatology. Published quarterly, the Journal of Cosmetic Dermatology facilitates continuing professional development and provides a forum for the exchange of scientific research and innovative techniques.
The scope of coverage includes, but will not be limited to: healthy skin; skin maintenance; ageing skin; photodamage and photoprotection; rejuvenation; biochemistry, endocrinology and neuroimmunology of healthy skin; imaging; skin measurement; quality of life; skin types; sensitive skin; rosacea and acne; sebum; sweat; fat; phlebology; hair conservation, restoration and removal; nails and nail surgery; pigment; psychological and medicolegal issues; retinoids; cosmetic chemistry; dermopharmacy; cosmeceuticals; toiletries; striae; cellulite; cosmetic dermatological surgery; blepharoplasty; liposuction; surgical complications; botulinum; fillers, peels and dermabrasion; local and tumescent anaesthesia; electrosurgery; lasers, including laser physics, laser research and safety, vascular lasers, pigment lasers, hair removal lasers, tattoo removal lasers, resurfacing lasers, dermal remodelling lasers and laser complications.