{"title":"SsCyp86通过调节脂肪酸代谢来调节孢子丝核菌的交配/丝化和致病性。","authors":"Enping Cai, Jiaru Deng, Ruqing Feng, Wenqiang Zheng, Yifang Wang, Meixin Yan, Changqing Chang","doi":"10.1080/21505594.2024.2395833","DOIUrl":null,"url":null,"abstract":"<p><strong>Background: </strong>Fatty acid metabolism constitutes a significant and intricate biochemical process within microorganisms. Cytochrome P450 (CYP450) enzymes are found in most organisms and occupy a pivotal position in the metabolism of fatty acids. However, the role of CYP450 enzyme mediated fatty acid metabolism in the pathogenicity of pathogenic fungi remains unclear.</p><p><strong>Methods: </strong>In this study, a CYP450 enzyme-encoding gene, <i>SsCYP86</i>, was identified in the sugarcane smut fungus <i>Sporisorium scitamineum</i> and its functions were characterized using a target gene homologous recombination strategy and metabonomics.</p><p><strong>Results: </strong>We found that the expression of <i>SsCYP86</i> was induced by or sugarcane wax or under the condition of mating/filamentation. Sexual reproduction assay demonstrated that the <i>SsCYP86</i> deletion mutant was defective in mating/filamentation and significantly reduced its pathogenicity. Further fatty acid metabolomic analysis unravelled the levels of fatty acid metabolites were reduced in the <i>SsCYP86</i> deletion mutant. Exogenous addition of fatty acid metabolites cis-11-eicosenoic acid (C20:1N9), pentadecanoic acid (C15:0), and linolenic acid (C18:3N3) partially restored the mating/filamentation ability of the <i>SsCYP86</i> deletion mutant and restored the transcriptional level of the <i>SsPRF1</i>, a pheromone response transcription factor that is typically down-regulated in the absence of <i>SsCYP86</i>. Moreover, the constitutive expression of <i>SsPRF1</i> in the <i>SsCYP86</i> deletion mutant restored its mating/filamentation.</p><p><strong>Conclusion: </strong>Our results indicated that SsCyp86 modulates the <i>SsPRF1</i> transcription by fatty acid metabolism, and thereby regulate the sexual reproduction of <i>S. scitamineum</i>. These findings provide insights into how CYPs regulate sexual reproduction in <i>S. scitamineum</i>.</p>","PeriodicalId":23747,"journal":{"name":"Virulence","volume":null,"pages":null},"PeriodicalIF":5.5000,"publicationDate":"2024-12-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://www.ncbi.nlm.nih.gov/pmc/articles/PMC11352786/pdf/","citationCount":"0","resultStr":"{\"title\":\"SsCyp86 modulates <i>sporisorium scitamineum</i> mating/filamentation and pathogenicity through regulating fatty acid metabolism.\",\"authors\":\"Enping Cai, Jiaru Deng, Ruqing Feng, Wenqiang Zheng, Yifang Wang, Meixin Yan, Changqing Chang\",\"doi\":\"10.1080/21505594.2024.2395833\",\"DOIUrl\":null,\"url\":null,\"abstract\":\"<p><strong>Background: </strong>Fatty acid metabolism constitutes a significant and intricate biochemical process within microorganisms. Cytochrome P450 (CYP450) enzymes are found in most organisms and occupy a pivotal position in the metabolism of fatty acids. However, the role of CYP450 enzyme mediated fatty acid metabolism in the pathogenicity of pathogenic fungi remains unclear.</p><p><strong>Methods: </strong>In this study, a CYP450 enzyme-encoding gene, <i>SsCYP86</i>, was identified in the sugarcane smut fungus <i>Sporisorium scitamineum</i> and its functions were characterized using a target gene homologous recombination strategy and metabonomics.</p><p><strong>Results: </strong>We found that the expression of <i>SsCYP86</i> was induced by or sugarcane wax or under the condition of mating/filamentation. Sexual reproduction assay demonstrated that the <i>SsCYP86</i> deletion mutant was defective in mating/filamentation and significantly reduced its pathogenicity. Further fatty acid metabolomic analysis unravelled the levels of fatty acid metabolites were reduced in the <i>SsCYP86</i> deletion mutant. Exogenous addition of fatty acid metabolites cis-11-eicosenoic acid (C20:1N9), pentadecanoic acid (C15:0), and linolenic acid (C18:3N3) partially restored the mating/filamentation ability of the <i>SsCYP86</i> deletion mutant and restored the transcriptional level of the <i>SsPRF1</i>, a pheromone response transcription factor that is typically down-regulated in the absence of <i>SsCYP86</i>. Moreover, the constitutive expression of <i>SsPRF1</i> in the <i>SsCYP86</i> deletion mutant restored its mating/filamentation.</p><p><strong>Conclusion: </strong>Our results indicated that SsCyp86 modulates the <i>SsPRF1</i> transcription by fatty acid metabolism, and thereby regulate the sexual reproduction of <i>S. scitamineum</i>. These findings provide insights into how CYPs regulate sexual reproduction in <i>S. scitamineum</i>.</p>\",\"PeriodicalId\":23747,\"journal\":{\"name\":\"Virulence\",\"volume\":null,\"pages\":null},\"PeriodicalIF\":5.5000,\"publicationDate\":\"2024-12-01\",\"publicationTypes\":\"Journal Article\",\"fieldsOfStudy\":null,\"isOpenAccess\":false,\"openAccessPdf\":\"https://www.ncbi.nlm.nih.gov/pmc/articles/PMC11352786/pdf/\",\"citationCount\":\"0\",\"resultStr\":null,\"platform\":\"Semanticscholar\",\"paperid\":null,\"PeriodicalName\":\"Virulence\",\"FirstCategoryId\":\"99\",\"ListUrlMain\":\"https://doi.org/10.1080/21505594.2024.2395833\",\"RegionNum\":1,\"RegionCategory\":\"农林科学\",\"ArticlePicture\":[],\"TitleCN\":null,\"AbstractTextCN\":null,\"PMCID\":null,\"EPubDate\":\"2024/8/27 0:00:00\",\"PubModel\":\"Epub\",\"JCR\":\"Q1\",\"JCRName\":\"IMMUNOLOGY\",\"Score\":null,\"Total\":0}","platform":"Semanticscholar","paperid":null,"PeriodicalName":"Virulence","FirstCategoryId":"99","ListUrlMain":"https://doi.org/10.1080/21505594.2024.2395833","RegionNum":1,"RegionCategory":"农林科学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":null,"EPubDate":"2024/8/27 0:00:00","PubModel":"Epub","JCR":"Q1","JCRName":"IMMUNOLOGY","Score":null,"Total":0}
SsCyp86 modulates sporisorium scitamineum mating/filamentation and pathogenicity through regulating fatty acid metabolism.
Background: Fatty acid metabolism constitutes a significant and intricate biochemical process within microorganisms. Cytochrome P450 (CYP450) enzymes are found in most organisms and occupy a pivotal position in the metabolism of fatty acids. However, the role of CYP450 enzyme mediated fatty acid metabolism in the pathogenicity of pathogenic fungi remains unclear.
Methods: In this study, a CYP450 enzyme-encoding gene, SsCYP86, was identified in the sugarcane smut fungus Sporisorium scitamineum and its functions were characterized using a target gene homologous recombination strategy and metabonomics.
Results: We found that the expression of SsCYP86 was induced by or sugarcane wax or under the condition of mating/filamentation. Sexual reproduction assay demonstrated that the SsCYP86 deletion mutant was defective in mating/filamentation and significantly reduced its pathogenicity. Further fatty acid metabolomic analysis unravelled the levels of fatty acid metabolites were reduced in the SsCYP86 deletion mutant. Exogenous addition of fatty acid metabolites cis-11-eicosenoic acid (C20:1N9), pentadecanoic acid (C15:0), and linolenic acid (C18:3N3) partially restored the mating/filamentation ability of the SsCYP86 deletion mutant and restored the transcriptional level of the SsPRF1, a pheromone response transcription factor that is typically down-regulated in the absence of SsCYP86. Moreover, the constitutive expression of SsPRF1 in the SsCYP86 deletion mutant restored its mating/filamentation.
Conclusion: Our results indicated that SsCyp86 modulates the SsPRF1 transcription by fatty acid metabolism, and thereby regulate the sexual reproduction of S. scitamineum. These findings provide insights into how CYPs regulate sexual reproduction in S. scitamineum.
期刊介绍:
Virulence is a fully open access peer-reviewed journal. All articles will (if accepted) be available for anyone to read anywhere, at any time immediately on publication.
Virulence is the first international peer-reviewed journal of its kind to focus exclusively on microbial pathogenicity, the infection process and host-pathogen interactions. To address the new infectious challenges, emerging infectious agents and antimicrobial resistance, there is a clear need for interdisciplinary research.