结合多刺激响应工程细菌和水凝胶分离平台进行多目标检测。

Journal of hazardous materials Pub Date : 2024-10-05 Epub Date: 2024-08-18 DOI:10.1016/j.jhazmat.2024.135578
Huachao Che, Xike Tian, Yulun Nie, Yong Li, Liqiang Lu, Yuguang Hu
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引用次数: 0

摘要

建立一种类似于 ICP-MS 的方法,能够同时、方便地原位定量分析多种重金属,这一点备受期待。在本研究中,我们整合了多个目标的传感元件和不同的荧光报告元件,构建了一种工程大肠杆菌。当这些靶标存在时,工程细菌可以发出相应波长的荧光信号。为了避免多个靶标共存时因荧光信号重叠而无法准确区分和量化每个靶标的含量,我们构建了一个类似于分离柱的水凝胶分离平台。该水凝胶平台可通过调节对不同目标物的吸附强度来改变检测限(LOD)和灵敏度,从而实现对各自检测信号的区分和识别。这种新检测方法对镉(II)、汞(II)、砷(III)和铅(II)的检测限分别为 1.249、0.380、3.917 和 0.755 μg/L。此外,该生物传感器系统还用于检测实际样品中共存的镉(II)、汞(II)、砷(III)和铅(II),回收率为 85.61-110.30%,与经典的 ICP-MS 检测结果一致,证实了该方法检测多种重金属共存样品的准确性和可靠性。
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Multi-targets detection via combination of multi-stimulus-response engineered bacteria and hydrogel-based separation platform.

Establishing a method similar to ICP-MS that can quantitatively analyze multiple heavy metals simultaneously, conveniently, and in situ is highly anticipated. In this study, we integrated the sensing elements of multiple targets and different fluorescence reporting elements to construct an engineered Escherichia coli. When these targets are present, the engineered bacteria can emit a fluorescent signal at the corresponding wavelength. To avoid the inability to accurately distinguish and quantify the content of each target due to the overlap of fluorescence signals when multiple targets coexist, a hydrogel-based separation platform similar to a separation column was constructed. The hydrogel platform can change the detection limit (LOD) and sensitivity by adjusting the adsorption strength towards different targets, so as to realize the differentiation and recognition of their respective detection signals. The LODs of this new detection method for Cd(II), Hg(II), As(III), and Pb(II) are 1.249, 0.380, 3.917, and 0.755 μg/L, respectively. In addition, this biosensor system was applied to detect coexisting Cd(II), Hg(II), As(III), and Pb(II) in actual samples with a recovery rate of 85.61-110.30 %, which is consistent with the classical ICP-MS detection results, confirming the accuracy and reliability of the method for detecting multiple heavy metal coexisting samples.

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