分析体外细胞活力法,为电化学疗法提供早期疗效预测

IF 3 Q2 ENGINEERING, ELECTRICAL & ELECTRONIC IEEE Journal of Electromagnetics RF and Microwaves in Medicine and Biology Pub Date : 2024-04-02 DOI:10.1109/JERM.2024.3379012
Anne Calvel;Alexia de Caro;Olivia Peytral-Rieu;Camille Gironde;Christophe Furger;David Dubuc;Katia Grenier;Marie-Pierre Rols
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引用次数: 0

摘要

近几十年来,在所有已开发的癌症治疗方法中,电化学疗法大有可为。这种方法将电脉冲的局部传递与渗透性较差的细胞毒剂的施用相结合。我们旨在研究电化学疗法的效果,并预测其对细胞活力的影响,尤其是在早期阶段。我们探索了不同的方法来评估细胞存活率,涉及的时间段从治疗后的几天到几小时不等。除了常用的克隆测定法和比色测定法,我们还研究了一种创新的活力测定法--Light Up 细胞系统测定法,并对这些方法进行了比较。我们的研究表明,比色法和 Light Up Cell System 检测法可在治疗后 2 小时内预测细胞对电化学疗法的反应,而评估细胞存活率的黄金标准--克隆检测法则需要 10 天的实验。此外,Light Up 细胞系统检测法似乎特别有趣,因为它能提供与成熟的比色法类似的结果,同时具有保持细胞活力和适合研究非粘附细胞系的优点。
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Analysis of In Vitro Cell Viability Approaches to Provide Early Efficacy Prediction of Electrochemotherapy Treatments
Among all the cancer treatments developed, electrochemotherapy has shown great promise in recent decades. This approach combines the local delivery of electric pulses with the administration of poorly-permeant cytotoxic agents. We aim to investigate the effects of electrochemotherapy treatments and predict their impacts on cell viability, especially at the earliest stage. We explore different approaches to evaluate cell viability, involving time periods from several days to few hours post treatment. Besides commonly-used approaches such as clonogenic and colorimetric assays, we investigate an innovative viability assay, the Light Up Cell System assay, and compare these methods. Even if the conducted viability assays demonstrate the interest of using electric fields to enhance the cytotoxic agent penetration into cells and potentiate their effects, our study demonstrates that the colorimetric and Light Up Cell System assays can predict the cell response to electrochemotherapy treatment as early as 2 hours post-treatment, whereas the gold standard for assessing cell viability, the clonogenic assay, necessitates 10 days of experimentation. Moreover, the Light Up Cell System assay seems particularly interesting, as it provides similar results to the well-established colorimetric technique while offering the advantages of maintaining cells alive and being suitable for the study of non-adherent cell lines.
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CiteScore
5.80
自引率
9.40%
发文量
58
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Front Cover Table of Contents IEEE Journal of Electromagnetics, RF, and Microwaves in Medicine and Biology About this Journal IEEE Journal of Electromagnetics, RF and Microwaves in Medicine and Biology Publication Information Front Cover
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