水稻小蛋白 OsS1Fa1 的跨膜结构域负责亚细胞定位和抗旱性。

IF 4.2 3区 生物学 Q1 PLANT SCIENCES Plant Biology Pub Date : 2024-08-23 DOI:10.1111/plb.13711
J. S. Kwak, K. H. Lee, W. K. Min, S. J. Lee, J. T. Song, H. S. Seo
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引用次数: 0

摘要

OsS1Fa1 是菠菜 S1Fa 的同源物,是水稻中的一种小蛋白,含有四个不同的保守基团,参与抗旱。然而,这些保守基团的生物学功能迄今尚未得到表征。因此,我们研究了这些保守结构域在 OsS1Fa1 的定位和细胞功能中的作用。我们使用共焦激光扫描显微镜(CLSM)分析了粒子轰击和细菌浸润后 OsS1Fa1 的亚细胞定位。我们进行了大肠杆菌体内重组苏木酰化试验,以研究 OsS1Fa1 的苏木酰化。我们利用转基因拟南芥植物鉴定了 OsS1Fa1 跨膜结构域在抗旱中的功能。荧光分析表明,OsS1Fa1定位于核膜和细胞质膜。突变和细胞分馏分析表明,膜定位结构域决定了 OsS1Fa1 的亚细胞定位。水稻同源物 OsS1Fa2 和拟南芥直向同源物 AtS1Fa1、AtS1Fa2 和 AtS1Fa3 也表现出与 OsS1Fa1 相似的定位模式。Sumoylation分析表明,OsS1Fa1与小泛素相关修饰物(SUMO)共轭。转基因分析表明,在拟南芥中过表达 OsS1Fa1 的跨膜结构域突变形式 OsS1Fa1(TMm1)并不能提高拟南芥对干旱胁迫的耐受性,而过表达 OsS1Fa1 则能提高转基因拟南芥的耐旱性。我们的数据表明,水稻和拟南芥的S1Fa1蛋白定位于核膜和细胞质膜,跨膜结构域决定亚细胞定位,并在干旱胁迫耐受性中发挥重要作用。
本文章由计算机程序翻译,如有差异,请以英文原文为准。

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The transmembrane domain of the rice small protein OsS1Fa1 is responsible for subcellular localization and drought tolerance

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来源期刊
Plant Biology
Plant Biology 生物-植物科学
CiteScore
8.20
自引率
2.60%
发文量
109
审稿时长
3 months
期刊介绍: Plant Biology is an international journal of broad scope bringing together the different subdisciplines, such as physiology, molecular biology, cell biology, development, genetics, systematics, ecology, evolution, ecophysiology, plant-microbe interactions, and mycology. Plant Biology publishes original problem-oriented full-length research papers, short research papers, and review articles. Discussion of hot topics and provocative opinion articles are published under the heading Acute Views. From a multidisciplinary perspective, Plant Biology will provide a platform for publication, information and debate, encompassing all areas which fall within the scope of plant science.
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