使用可见光和近红外线在体内测量光生物调制后完整人体皮肤释放的一氧化氮:化学发光检测研究

Augustin C. Barolet , Lucie Germain , Daniel Barolet
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引用次数: 0

摘要

意义重大:本研究探讨了利用可见光和近红外光进行光生物调节(PBM)对完整人体皮肤释放一氧化氮(NO)的治疗潜力。鉴于一氧化氮在伤口愈合、炎症控制和血管扩张等生理过程中的关键作用,这项研究可能会带来创新的非侵入性治疗方法。方法:18 名健康参与者在前臂上涂抹了一层PBM,并在前臂上安装了一个带有气孔的定制密闭套,用于测量NO 水平,评估三种特定波长的光(455 nm、660 nm 和 850 nm)对NO 的影响:方法:18 名健康参与者的前臂被包裹在密闭套筒中。方法:将 18 名健康参与者的前臂包裹在密闭的袖子中,用指定波长的光以 45 J/cm²、50 mW/cm² 的辐照度照射皮肤 15 分钟。利用化学发光检测(CLD)对照射后的 NO 含量进行量化,该方法测量 NO 与臭氧(O3)的化学发光反应,以进行实时分析:结果:在不同波长的测试中观察到 NO 释放量的显著差异,表明 PBM 能刺激完整人体皮肤释放 NO:这项研究提供了强有力的证据,证明使用可见光和近红外光的 PBM 可以增强人体皮肤的 NO 释放,从而为涉及 NO 的疾病提供了潜在的治疗应用。要了解 PBM 诱导 NO 释放背后的机制及其临床意义,还需要进一步的研究。
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In vivo measurement of nitric oxide release from intact human skin post photobiomodulation using visible and near-infrared light: A chemiluminescence detection study

Significance: This study investigates the therapeutic potential of photobiomodulation (PBM) using visible and near-infrared (NIR) light on nitric oxide (NO) release from intact human skin. Given NO's critical role in physiological processes such as wound healing, inflammation control, and vasodilation, this research could lead to innovative non-invasive treatments.

Aim: The primary aim was to explore how PBM at different wavelengths affects NO release from human skin. Custom-built airtight sleeves equipped with gas ports were used to measure NO levels, assessing the impact of three specific wavelengths of light (455 nm, 660 nm, and 850 nm).

Approach: Eighteen healthy participants had their forearms enclosed in airtight sleeves. The skin was irradiated with the specified wavelengths at a fluence of 45 J/cm² and an irradiance of 50 mW/cm² for 15 min. NO levels were quantified after irradiation using chemiluminescence detection (CLD), which measures the chemiluminescent reaction of NO with ozone (O3) for real-time analysis.

Results: Significant differences in NO release were observed among the wavelengths tested, indicating that PBM stimulates NO release from intact human skin.

Conclusions: The study provides strong evidence that PBM using visible and NIR light can enhance NO release from human skin, suggesting potential therapeutic applications for conditions involving NO. Further research is needed to understand the mechanisms behind PBM-induced NO release and its clinical implications.

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