维生素 D3 的应用及其在雄性大鼠肾上腺皮质中的活性因素:一项功能组织学研究。

IF 2 3区 医学 Q2 ANATOMY & MORPHOLOGY Annals of Anatomy-Anatomischer Anzeiger Pub Date : 2024-08-23 DOI:10.1016/j.aanat.2024.152322
Vladimir Ajdžanović , Branka Šošić-Jurjević , Jasmina Živanović , Marko Miler , Sanja Stanković , Jovan Ranin , Branko Filipović
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引用次数: 0

摘要

引言和目的:在衰老人群中补充维生素 D 对各种健康相关参数有积极影响。我们对大鼠肾上腺皮质进行了功能组织学分析,研究了应用维生素 D3 后大鼠肾上腺皮质的维生素 D 活性和皮质酮输出的因素:中年Wistar大鼠分为假手术组(SO;n=8)、睾丸切除组(Orx;n=8)和维生素D3治疗睾丸切除组(Orx+维生素D;n=8)。维生素 D3(5μg/kg b.m.)皮下注射三周,而 SO 组和 Orx 组仅接受药物治疗。通过组织化学/免疫组化、立体学、超微结构和生化分析实现了设定的目标:结果:睾丸切除术(Orx)降低了肾上腺皮质相关血管(pC)的体积密度:对雄激素过多的大鼠施用维生素 D3 后皮质酮输出量增加似乎与肾上腺组织中 25- 羟维生素 D3 的可用性增加和 1,25-二羟维生素 D3 的降解减少无关,而是与中枢调节机制有关。
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Vitamin D3 application and factors of its activity in the adrenal cortex of andropausal rats: A functionally-histological study

Introduction and aim

Vitamin D supplementation in aging subjects manifests a positive effect on various health-related parameters. We performed a functionally-histological analysis of the adrenal cortex regarding the factors of vitamin D activity and corticosterone output after vitamin D3 application in a rat model of the andropause.

Material and methods

Middle-aged Wistar rats were divided into sham operated (SO; n=8), orchidectomized (Orx; n=8) and vitamin D3-treated orchidectomized (Orx+vit. D; n=8) groups. Vitamin D3 (5 μg/kg b.m.) was administered subcutaneously for three weeks, while the SO and Orx groups received the vehicle alone. Set objectives were achieved using histochemistry/immunohistochemistry, stereology, ultrastructural and biochemical analyses.

Results

Orchidectomy (Orx) decreased the adrenal cortex-related volume densities of vascular (p<0,0001), vitamin D receptor (VDR; p<0,0166), cytochrome P450 oxidase 2R1 (CYP 2R1; p<0,0001) and cytochrome P450 oxidase 24 (CYP 24; p<0,0001) depots, but increased the volume density of cytochrome P450 27B1 (CYP 27B1; p<0,0001) depots. In Orx+vit. D rats, increase of the adrenal cortex-related volume densities of collagen (p<0,0001), VDR (p<0,0001) and CYP 2R1 (p<0,0001) depots as well as the lipid-droplet diameter (p<0,0001) in adrenocortical outer zona fasciculata cells was observed, while a decrease of volume densities of the vascular (p<0,0001), CYP 27B1 (p<0,0001) and CYP 24 (p<0,0001) depots was registered, all versus Orx group. Plasma level of ACTH was decreased (p=0,0155) and serum concentrations of 25-hydroxyvitamin D3 and corticosterone were increased (p<0,0001 and p=0,0187, respectively), all after the same treatment.

Conclusions

Increased corticosterone output after vitamin D3 application to andropausal rats appears not to be related to increased availability of 25-hydroxyvitamin D3 and decreased degradation of 1,25-dihydroxyvitamin D3 in adrenal tissue, but rather involves the central regulatory mechanisms.

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来源期刊
Annals of Anatomy-Anatomischer Anzeiger
Annals of Anatomy-Anatomischer Anzeiger 医学-解剖学与形态学
CiteScore
4.40
自引率
22.70%
发文量
137
审稿时长
33 days
期刊介绍: Annals of Anatomy publish peer reviewed original articles as well as brief review articles. The journal is open to original papers covering a link between anatomy and areas such as •molecular biology, •cell biology •reproductive biology •immunobiology •developmental biology, neurobiology •embryology as well as •neuroanatomy •neuroimmunology •clinical anatomy •comparative anatomy •modern imaging techniques •evolution, and especially also •aging
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