伊朗西部霍拉马巴德流浪猫粪便中弓形虫的分子检测和基因分型

IF 1.9 Q2 AGRICULTURE, DAIRY & ANIMAL SCIENCE Veterinary and Animal Science Pub Date : 2024-08-15 DOI:10.1016/j.vas.2024.100389
Hakim Azizi , Maryam Hataminejad , Ali Taghipour , Maryam Norouzi , Aliyar Mirzapour
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引用次数: 0

摘要

猫是弓形虫的最终宿主,对这种寄生虫的传播和爆发有着重要影响。了解这种寄生虫传播模式的一个重要因素是分析感染弓形虫的猫的基因多样性分布。本研究旨在确定伊朗西部霍拉马巴德流浪猫粪便中淋病双球菌的流行率和基因分型。在 2016-2017 年期间,对 200 只猫进行了采样,以获得新鲜粪便标本。利用寄生虫学方法对卵囊进行鉴定。使用市售基因组迷你试剂盒从粪便中分离 DNA。为了确定淋球菌的基因组成,我们对 GRA6 目标基因进行了 PCR-RFLP、测序和系统进化分析。所有样本均未通过寄生虫学技术检测。采用 GRA6-PCR 方法检测时,共有 6.5 %(13/200)的样本呈阳性。根据 PCR-RFLP 结果,所有 13 个样本均为淋球菌 III 型基因型。本研究发现,两个样本的核苷酸序列与 12 个参照的淋病双球菌和一个用作外部对照的 Hammondia hamondi 菌株的核苷酸序列相差 5%。根据研究结果,分子检测比寄生虫学方法更灵敏。RFLP 方法显示,在伊朗西部的霍拉马巴德,淋病双球菌的 III 型是普遍和重要的基因型。
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Molecular detection and genotyping of Toxoplasma gondii in stray cat feces from Khorramabad, West Iran

Cats, being the definitive host of Toxoplasma gondii, have a significant impact on the spread and outbreaks of the parasite. An essential factor in comprehending the transmission pattern of this parasite is an analysis of the genetic diversity distribution in cats infected with T. gondii. This study was aimed at determining the prevalence rate and genotyping of T. gondii in stray cat feces from Khorramabad, West Iran. In the years 2016–2017, 200 cats were sampled to get fresh feces specimens. Parasitological methods were utilized for the identification of oocysts. The DNA was isolated from the feces using a commercially available Genomic Mini Kit. In order to identify the genetic composition of T. gondii, we employed PCR-RFLP, sequencing, and phylogenetic analysis of the GRA6 target gene. No one of the samples tested positive for parasitology techniques. A total of 6.5 % (13/200) samples were positive when using the GRA6-PCR method. Based on PCR-RFLP results, all 13 samples were of T. gondii type III genotype. The nucleotide sequences of two samples from this study were found to be 5 % different from those of 12 references of T. gondii and one strain of Hammondia hamondi that was used as an external control. Based on the findings, molecular tests are more sensitive than parasitological methods. The RFLP approach revealed that type III of T. gondii is the prevailing and important genotype in Khorramabad, West Iran.

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来源期刊
Veterinary and Animal Science
Veterinary and Animal Science Veterinary-Veterinary (all)
CiteScore
3.50
自引率
0.00%
发文量
43
审稿时长
47 days
期刊最新文献
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