通过控制金/氧化铝平台的粗糙度开发基于表面增强拉曼光谱的新型生物传感器,用于高灵敏度检测铜绿假单胞菌分泌的焦花青素。

IF 4.9 3区 工程技术 Q1 CHEMISTRY, ANALYTICAL Biosensors-Basel Pub Date : 2024-08-19 DOI:10.3390/bios14080399
Waleed A El-Said, Tamer S Saleh, Abdullah Saad Al-Bogami, Mohmmad Younus Wani, Jeong-Woo Choi
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引用次数: 0

摘要

焦花青素被认为是铜绿假单胞菌(P. aeruginosa)感染的制造者。焦花青素是铜绿假单胞菌释放的毒素之一。因此,鉴于PYO的重要性,开发直接检测PYO的方法至关重要。在各种光学技术中,拉曼技术因其指纹数据、无需样品制备、灵敏度高以及使用方便等优点而显示出独特的优势。基于表面增强拉曼散射(SERS)技术,贵金属纳米结构被用来改善拉曼响应。阳极金属氧化物因其独特的形态和应用而备受关注。多孔金属结构具有较大的表面积,可用作周期性纳米结构阵列制造的硬模板。除了金属纯度和电解液类型/浓度之外,还可以通过控制阳极氧化参数(包括阳极氧化电压、电流、温度和时间)来控制多孔的形状和大小。铝箔阳极氧化会形成不同粗糙度的阳极氧化铝(AAO)。在此,我们将利用粗糙度作为热点中心来增强拉曼信号。首先,沉积一层金薄膜来开发金/氧化铝(Au/AAO)平台,然后将其用作 SERS 活性表面。使用扫描电子显微镜(SEM)和原子力显微镜(AFM)技术研究了所开发基底的形态和粗糙度。基于 SERS 技术,将 Au/AAO 基底用于监测铜绿假单胞菌微生物分泌的焦花青素。结果表明,粗糙度会影响该传感器的增强效率。在二次阳极氧化基底上沉积 30 nm 金层时,传感器的增强效率较高。所开发的传感器对焦花青素具有很高的灵敏度,检测限为 96 nM,在 1 µM 至 9 µM 的动态范围内呈线性响应。
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Development of Novel Surface-Enhanced Raman Spectroscopy-Based Biosensors by Controlling the Roughness of Gold/Alumina Platforms for Highly Sensitive Detection of Pyocyanin Secreted from Pseudomonas aeruginosa.

Pyocyanin is considered a maker of Pseudomonas aeruginosa (P. aeruginosa) infection. Pyocyanin is among the toxins released by the P. aeruginosa bacteria. Therefore, the development of a direct detection of PYO is crucial due to its importance. Among the different optical techniques, the Raman technique showed unique advantages because of its fingerprint data, no sample preparation, and high sensitivity besides its ease of use. Noble metal nanostructures were used to improve the Raman response based on the surface-enhanced Raman scattering (SERS) technique. Anodic metal oxide attracts much interest due to its unique morphology and applications. The porous metal structure provides a large surface area that could be used as a hard template for periodic nanostructure array fabrication. Porous shapes and sizes could be controlled by controlling the anodization parameters, including the anodization voltage, current, temperature, and time, besides the metal purity and the electrolyte type/concentration. The anodization of aluminum foil results in anodic aluminum oxide (AAO) formation with different roughness. Here, we will use the roughness as hotspot centers to enhance the Raman signals. Firstly, a thin film of gold was deposited to develop gold/alumina (Au/AAO) platforms and then applied as SERS-active surfaces. The morphology and roughness of the developed substrates were investigated using scanning electron microscopy (SEM) and atomic force microscopy (AFM) techniques. The Au/AAO substrates were used for monitoring pyocyanin secreted from Pseudomonas aeruginosa microorganisms based on the SERS technique. The results showed that the roughness degree affects the enhancement efficiency of this sensor. The high enhancement was obtained in the case of depositing a 30 nm layer of gold onto the second anodized substrates. The developed sensor showed high sensitivity toward pyocyanin with a limit of detection of 96 nM with a linear response over a dynamic range from 1 µM to 9 µM.

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来源期刊
Biosensors-Basel
Biosensors-Basel Biochemistry, Genetics and Molecular Biology-Clinical Biochemistry
CiteScore
6.60
自引率
14.80%
发文量
983
审稿时长
11 weeks
期刊介绍: Biosensors (ISSN 2079-6374) provides an advanced forum for studies related to the science and technology of biosensors and biosensing. It publishes original research papers, comprehensive reviews and communications. Our aim is to encourage scientists to publish their experimental and theoretical results in as much detail as possible. There is no restriction on the length of the papers. The full experimental details must be provided so that the results can be reproduced. Electronic files and software regarding the full details of the calculation or experimental procedure, if unable to be published in a normal way, can be deposited as supplementary electronic material.
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