活体微藻细胞的多波长光声多普勒流量计。

IF 4.9 3区 工程技术 Q1 CHEMISTRY, ANALYTICAL Biosensors-Basel Pub Date : 2024-08-16 DOI:10.3390/bios14080397
Tayyab Farooq, Xiuru Wu, Sheng Yan, Hui Fang
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引用次数: 0

摘要

根据微藻体内的光合作用色素,光声学可以直接测量微藻对光的吸收。在这项研究中,我们对活体微藻细胞进行了光声流动测量,通过观察其流动状态下的光声多普勒功率谱,测量其流动特性,包括流速、流动角度、流动方向,以及更重要的光声吸收光谱。光源采用了高重复频率的超连续脉冲激光器:通过在指定频率上的强度调制,它可以提供以该频率为中心的光声信号的波长可选激励。我们的方法可用于同时测量微藻的流动特性,并在静态和动态状态下高精度地分辨出不同种类的微藻,从而促进对微藻培养及其在生态系统中作用的研究。
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Multiwavelength Photoacoustic Doppler Flowmetry of Living Microalgae Cells.

Photoacoustics can provide a direct measurement of light absorption by microalgae depending on the photosynthesis pigment within them. In this study, we have performed photoacoustic flowmetry on living microalgae cells to measure their flow characteristics, which include flow speed, flow angle, flow direction, and, more importantly, the photoacoustic absorption spectrum, all by observing the photoacoustic Doppler power spectra during their flowing state. A supercontinuum pulsed laser with a high repetition frequency is used as the light source: through intensity modulation at a specified frequency, it can provide wavelength-selectable excitation of a photoacoustic signal centered around this frequency. Our approach can be useful to simultaneously measure the flow characteristics of microalgae and easily discriminate their different species with high accuracy in both static and dynamic states, thus facilitating the study of their cultivation and their role in our ecosystem.

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来源期刊
Biosensors-Basel
Biosensors-Basel Biochemistry, Genetics and Molecular Biology-Clinical Biochemistry
CiteScore
6.60
自引率
14.80%
发文量
983
审稿时长
11 weeks
期刊介绍: Biosensors (ISSN 2079-6374) provides an advanced forum for studies related to the science and technology of biosensors and biosensing. It publishes original research papers, comprehensive reviews and communications. Our aim is to encourage scientists to publish their experimental and theoretical results in as much detail as possible. There is no restriction on the length of the papers. The full experimental details must be provided so that the results can be reproduced. Electronic files and software regarding the full details of the calculation or experimental procedure, if unable to be published in a normal way, can be deposited as supplementary electronic material.
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