{"title":"贵贝 MKK 家族基因的特征及其对温度胁迫和副溶血性弧菌感染的反应","authors":"","doi":"10.1016/j.marenvres.2024.106695","DOIUrl":null,"url":null,"abstract":"<div><p>Mitogen-activated protein kinase kinase (MKK), the key element of the Mitogen-activated protein kinase (MAPK) signaling pathway, is crucial for the immune response to adverse environments in aquatic animals. Nevertheless, there is limited information regarding the role of the <em>MKK</em> gene family in mollusks. In our study, genome data and transcriptome were used to identify four <em>MKK</em> genes (<em>CnMKK4</em>, <em>CnMKK5</em>, <em>CnMKK6</em>, and <em>CnMKK7</em>) in the noble scallop. The result of the gene structure, motif analysis, and phylogenetic tree revealed that <em>MKK</em> genes are relatively conserved in bivalves. Moreover, four <em>CnMKK</em> genes were significantly highly expressed in immune-related tissues, suggesting that <em>CnMKKs</em> may related to bivalve immunity. Furthermore, <em>CnMKK6</em> and <em>CgMKK4</em> were significantly differentially expressed (<em>P</em> < 0.05) under 24 h of temperature stress, and all <em>CnMKKs</em> were significantly differentially expressed (<em>P</em> < 0.05) under 24 h of <em>Vibrio parahaemolyticus</em> infection. These results showed that the <em>CnMKKs</em> may have a significant impact under biotic and abiotic stresses. In conclusion, the result of the <em>CnMKKs</em> provides valuable insights into comprehending the function of MKK genes in mollusks.</p></div>","PeriodicalId":18204,"journal":{"name":"Marine environmental research","volume":null,"pages":null},"PeriodicalIF":3.0000,"publicationDate":"2024-08-20","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":"0","resultStr":"{\"title\":\"Characterization of MKK family genes and their responses to temperature stress and Vibrio parahaemolyticus infection in noble scallop Chlamys nobilis\",\"authors\":\"\",\"doi\":\"10.1016/j.marenvres.2024.106695\",\"DOIUrl\":null,\"url\":null,\"abstract\":\"<div><p>Mitogen-activated protein kinase kinase (MKK), the key element of the Mitogen-activated protein kinase (MAPK) signaling pathway, is crucial for the immune response to adverse environments in aquatic animals. Nevertheless, there is limited information regarding the role of the <em>MKK</em> gene family in mollusks. In our study, genome data and transcriptome were used to identify four <em>MKK</em> genes (<em>CnMKK4</em>, <em>CnMKK5</em>, <em>CnMKK6</em>, and <em>CnMKK7</em>) in the noble scallop. The result of the gene structure, motif analysis, and phylogenetic tree revealed that <em>MKK</em> genes are relatively conserved in bivalves. Moreover, four <em>CnMKK</em> genes were significantly highly expressed in immune-related tissues, suggesting that <em>CnMKKs</em> may related to bivalve immunity. Furthermore, <em>CnMKK6</em> and <em>CgMKK4</em> were significantly differentially expressed (<em>P</em> < 0.05) under 24 h of temperature stress, and all <em>CnMKKs</em> were significantly differentially expressed (<em>P</em> < 0.05) under 24 h of <em>Vibrio parahaemolyticus</em> infection. These results showed that the <em>CnMKKs</em> may have a significant impact under biotic and abiotic stresses. In conclusion, the result of the <em>CnMKKs</em> provides valuable insights into comprehending the function of MKK genes in mollusks.</p></div>\",\"PeriodicalId\":18204,\"journal\":{\"name\":\"Marine environmental research\",\"volume\":null,\"pages\":null},\"PeriodicalIF\":3.0000,\"publicationDate\":\"2024-08-20\",\"publicationTypes\":\"Journal Article\",\"fieldsOfStudy\":null,\"isOpenAccess\":false,\"openAccessPdf\":\"\",\"citationCount\":\"0\",\"resultStr\":null,\"platform\":\"Semanticscholar\",\"paperid\":null,\"PeriodicalName\":\"Marine environmental research\",\"FirstCategoryId\":\"93\",\"ListUrlMain\":\"https://www.sciencedirect.com/science/article/pii/S0141113624003568\",\"RegionNum\":3,\"RegionCategory\":\"环境科学与生态学\",\"ArticlePicture\":[],\"TitleCN\":null,\"AbstractTextCN\":null,\"PMCID\":null,\"EPubDate\":\"\",\"PubModel\":\"\",\"JCR\":\"Q2\",\"JCRName\":\"ENVIRONMENTAL SCIENCES\",\"Score\":null,\"Total\":0}","platform":"Semanticscholar","paperid":null,"PeriodicalName":"Marine environmental research","FirstCategoryId":"93","ListUrlMain":"https://www.sciencedirect.com/science/article/pii/S0141113624003568","RegionNum":3,"RegionCategory":"环境科学与生态学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":null,"EPubDate":"","PubModel":"","JCR":"Q2","JCRName":"ENVIRONMENTAL SCIENCES","Score":null,"Total":0}
Characterization of MKK family genes and their responses to temperature stress and Vibrio parahaemolyticus infection in noble scallop Chlamys nobilis
Mitogen-activated protein kinase kinase (MKK), the key element of the Mitogen-activated protein kinase (MAPK) signaling pathway, is crucial for the immune response to adverse environments in aquatic animals. Nevertheless, there is limited information regarding the role of the MKK gene family in mollusks. In our study, genome data and transcriptome were used to identify four MKK genes (CnMKK4, CnMKK5, CnMKK6, and CnMKK7) in the noble scallop. The result of the gene structure, motif analysis, and phylogenetic tree revealed that MKK genes are relatively conserved in bivalves. Moreover, four CnMKK genes were significantly highly expressed in immune-related tissues, suggesting that CnMKKs may related to bivalve immunity. Furthermore, CnMKK6 and CgMKK4 were significantly differentially expressed (P < 0.05) under 24 h of temperature stress, and all CnMKKs were significantly differentially expressed (P < 0.05) under 24 h of Vibrio parahaemolyticus infection. These results showed that the CnMKKs may have a significant impact under biotic and abiotic stresses. In conclusion, the result of the CnMKKs provides valuable insights into comprehending the function of MKK genes in mollusks.
期刊介绍:
Marine Environmental Research publishes original research papers on chemical, physical, and biological interactions in the oceans and coastal waters. The journal serves as a forum for new information on biology, chemistry, and toxicology and syntheses that advance understanding of marine environmental processes.
Submission of multidisciplinary studies is encouraged. Studies that utilize experimental approaches to clarify the roles of anthropogenic and natural causes of changes in marine ecosystems are especially welcome, as are those studies that represent new developments of a theoretical or conceptual aspect of marine science. All papers published in this journal are reviewed by qualified peers prior to acceptance and publication. Examples of topics considered to be appropriate for the journal include, but are not limited to, the following:
– The extent, persistence, and consequences of change and the recovery from such change in natural marine systems
– The biochemical, physiological, and ecological consequences of contaminants to marine organisms and ecosystems
– The biogeochemistry of naturally occurring and anthropogenic substances
– Models that describe and predict the above processes
– Monitoring studies, to the extent that their results provide new information on functional processes
– Methodological papers describing improved quantitative techniques for the marine sciences.