{"title":"用连续微波非等温加热巴氏杀菌法对榛子(Aegle marmelos)饮料进行巴氏杀菌,以实现微生物安全","authors":"Rishab Dhar, Snehasis Chakraborty","doi":"10.1016/j.ifset.2024.103801","DOIUrl":null,"url":null,"abstract":"<div><p>The efficacy of conventional thermal (batch) and continuous microwave (MW) heating to pasteurize bael beverage was examined by inactivating <em>Escherichia coli</em>, <em>Listeria monocytogenes</em>, <em>Salmonella</em> Typhimurium, <em>Saccharomyces cerevisiae</em>, their cocktail, and native microbial groups. Continuous-MW was operated at 2 kW power and 45–80 °C final temperature. For 25–90 °C beverage temperature, the fluid followed a laminar flow. Data were appropriately modelled using first-order kinetics. MW-heating effectively inactivated all microorganisms. <em>S. cerevisiae</em> was most resistant towards thermal inactivation under single (<em>D</em><sub><em>70 °C</em></sub> = 0.03 min) and cocktail scenario (<em>D</em><sub><em>80 °C</em></sub> = 0.014 min) and towards MW under cocktail scenario (<em>D</em><sub><em>MW_67.5 °C</em></sub> = 0.23 min). The resistance towards temperature rise was highest by <em>L. monocytogenes</em>. Based on lower <em>z</em><sub><em>MW</em></sub>-values, the thermal sensitivity of microbes improved under MW heating. Morphological alteration in microbial cells of <em>S. cerevisiae</em> & <em>L. monocytogens</em> reveals that the lethality of MW inactivation is mainly based on its thermal effects.</p></div><div><h3>Industrial relevance</h3><p><em>Bael</em> fruit is a nutritious tropical fruit whose beverage is relished by consumers for its refreshing flavour and numerous health benefits. The food sector is presently concentrating on creating innovative, minimally processed goods that are ready-to-eat or handy, shelf-stable, and of higher quality. As an alternative to conventional thermal processing, several novel and sustainable processing methods are now being investigated. To determine if microwave processing is a suitable substitute for thermal processing, the inactivation of artificially inoculated microorganisms, their cocktail, and indigenous microorganisms in the bael beverage were examined in this work. Kinetic study, beverage rheology and changes in cell morphology were also studied. The study's findings indicate that continuous microwave technology can be a more suitable method of pasteurizing fruit beverages than conventional heating due to its >7 log reduction in a shorter time. Except for a few differences, the inactivation mechanisms of thermal and microwave are comparable.</p></div>","PeriodicalId":329,"journal":{"name":"Innovative Food Science & Emerging Technologies","volume":"97 ","pages":"Article 103801"},"PeriodicalIF":6.3000,"publicationDate":"2024-08-22","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":"0","resultStr":"{\"title\":\"Bael (Aegle marmelos) beverage pasteurization by non-isothermal heating with continuous microwave to achieve microbial safety\",\"authors\":\"Rishab Dhar, Snehasis Chakraborty\",\"doi\":\"10.1016/j.ifset.2024.103801\",\"DOIUrl\":null,\"url\":null,\"abstract\":\"<div><p>The efficacy of conventional thermal (batch) and continuous microwave (MW) heating to pasteurize bael beverage was examined by inactivating <em>Escherichia coli</em>, <em>Listeria monocytogenes</em>, <em>Salmonella</em> Typhimurium, <em>Saccharomyces cerevisiae</em>, their cocktail, and native microbial groups. Continuous-MW was operated at 2 kW power and 45–80 °C final temperature. For 25–90 °C beverage temperature, the fluid followed a laminar flow. Data were appropriately modelled using first-order kinetics. MW-heating effectively inactivated all microorganisms. <em>S. cerevisiae</em> was most resistant towards thermal inactivation under single (<em>D</em><sub><em>70 °C</em></sub> = 0.03 min) and cocktail scenario (<em>D</em><sub><em>80 °C</em></sub> = 0.014 min) and towards MW under cocktail scenario (<em>D</em><sub><em>MW_67.5 °C</em></sub> = 0.23 min). The resistance towards temperature rise was highest by <em>L. monocytogenes</em>. Based on lower <em>z</em><sub><em>MW</em></sub>-values, the thermal sensitivity of microbes improved under MW heating. Morphological alteration in microbial cells of <em>S. cerevisiae</em> & <em>L. monocytogens</em> reveals that the lethality of MW inactivation is mainly based on its thermal effects.</p></div><div><h3>Industrial relevance</h3><p><em>Bael</em> fruit is a nutritious tropical fruit whose beverage is relished by consumers for its refreshing flavour and numerous health benefits. The food sector is presently concentrating on creating innovative, minimally processed goods that are ready-to-eat or handy, shelf-stable, and of higher quality. As an alternative to conventional thermal processing, several novel and sustainable processing methods are now being investigated. To determine if microwave processing is a suitable substitute for thermal processing, the inactivation of artificially inoculated microorganisms, their cocktail, and indigenous microorganisms in the bael beverage were examined in this work. Kinetic study, beverage rheology and changes in cell morphology were also studied. The study's findings indicate that continuous microwave technology can be a more suitable method of pasteurizing fruit beverages than conventional heating due to its >7 log reduction in a shorter time. Except for a few differences, the inactivation mechanisms of thermal and microwave are comparable.</p></div>\",\"PeriodicalId\":329,\"journal\":{\"name\":\"Innovative Food Science & Emerging Technologies\",\"volume\":\"97 \",\"pages\":\"Article 103801\"},\"PeriodicalIF\":6.3000,\"publicationDate\":\"2024-08-22\",\"publicationTypes\":\"Journal Article\",\"fieldsOfStudy\":null,\"isOpenAccess\":false,\"openAccessPdf\":\"\",\"citationCount\":\"0\",\"resultStr\":null,\"platform\":\"Semanticscholar\",\"paperid\":null,\"PeriodicalName\":\"Innovative Food Science & Emerging Technologies\",\"FirstCategoryId\":\"97\",\"ListUrlMain\":\"https://www.sciencedirect.com/science/article/pii/S1466856424002406\",\"RegionNum\":1,\"RegionCategory\":\"农林科学\",\"ArticlePicture\":[],\"TitleCN\":null,\"AbstractTextCN\":null,\"PMCID\":null,\"EPubDate\":\"\",\"PubModel\":\"\",\"JCR\":\"Q1\",\"JCRName\":\"FOOD SCIENCE & TECHNOLOGY\",\"Score\":null,\"Total\":0}","platform":"Semanticscholar","paperid":null,"PeriodicalName":"Innovative Food Science & Emerging Technologies","FirstCategoryId":"97","ListUrlMain":"https://www.sciencedirect.com/science/article/pii/S1466856424002406","RegionNum":1,"RegionCategory":"农林科学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":null,"EPubDate":"","PubModel":"","JCR":"Q1","JCRName":"FOOD SCIENCE & TECHNOLOGY","Score":null,"Total":0}
引用次数: 0
摘要
通过灭活大肠杆菌、单核细胞增生李斯特菌、鼠伤寒沙门氏菌、酿酒酵母菌、它们的混合菌群和本地微生物群,研究了传统热加热(间歇式)和连续微波(MW)加热对薏仁饮料巴氏杀菌的功效。连续式水处理机的功率为 2 千瓦,最终温度为 45-80 °C。在 25-90 °C 的饮料温度下,流体为层流。使用一阶动力学对数据进行了适当建模。兆瓦加热可有效灭活所有微生物。在单一方案(D70 °C = 0.03 分钟)和鸡尾酒方案(D80 °C = 0.014 分钟)下,麦角菌对热失活的抵抗力最强;在鸡尾酒方案(DMW_67.5 °C = 0.23 分钟)下,麦角菌对 MW 的抵抗力最强。单核细胞增生菌对温度升高的抵抗力最强。根据较低的 zMW 值,微生物的热敏感性在 MW 加热条件下有所提高。单核细胞增多症微生物细胞形态的改变表明,MW 失活的致死性主要基于其热效应。 工业相关性白果是一种营养丰富的热带水果,其饮料因其清爽的口感和众多健康益处而深受消费者喜爱。目前,食品行业正专注于生产创新型的微加工产品,这些产品可即食或方便食用,可在货架上保存,而且质量更高。作为传统热加工的替代方法,目前正在研究几种新型的可持续加工方法。为了确定微波加工是否适合替代热加工,这项工作研究了人工接种的微生物、其鸡尾酒和菠萝饮料中本地微生物的失活情况。此外,还对动力学研究、饮料流变学和细胞形态变化进行了研究。研究结果表明,与传统加热相比,连续微波技术可以在更短的时间内降低 7 个对数值,是一种更合适的水果饮料巴氏杀菌方法。除少数差异外,热力和微波的灭活机制不相上下。
Bael (Aegle marmelos) beverage pasteurization by non-isothermal heating with continuous microwave to achieve microbial safety
The efficacy of conventional thermal (batch) and continuous microwave (MW) heating to pasteurize bael beverage was examined by inactivating Escherichia coli, Listeria monocytogenes, Salmonella Typhimurium, Saccharomyces cerevisiae, their cocktail, and native microbial groups. Continuous-MW was operated at 2 kW power and 45–80 °C final temperature. For 25–90 °C beverage temperature, the fluid followed a laminar flow. Data were appropriately modelled using first-order kinetics. MW-heating effectively inactivated all microorganisms. S. cerevisiae was most resistant towards thermal inactivation under single (D70 °C = 0.03 min) and cocktail scenario (D80 °C = 0.014 min) and towards MW under cocktail scenario (DMW_67.5 °C = 0.23 min). The resistance towards temperature rise was highest by L. monocytogenes. Based on lower zMW-values, the thermal sensitivity of microbes improved under MW heating. Morphological alteration in microbial cells of S. cerevisiae & L. monocytogens reveals that the lethality of MW inactivation is mainly based on its thermal effects.
Industrial relevance
Bael fruit is a nutritious tropical fruit whose beverage is relished by consumers for its refreshing flavour and numerous health benefits. The food sector is presently concentrating on creating innovative, minimally processed goods that are ready-to-eat or handy, shelf-stable, and of higher quality. As an alternative to conventional thermal processing, several novel and sustainable processing methods are now being investigated. To determine if microwave processing is a suitable substitute for thermal processing, the inactivation of artificially inoculated microorganisms, their cocktail, and indigenous microorganisms in the bael beverage were examined in this work. Kinetic study, beverage rheology and changes in cell morphology were also studied. The study's findings indicate that continuous microwave technology can be a more suitable method of pasteurizing fruit beverages than conventional heating due to its >7 log reduction in a shorter time. Except for a few differences, the inactivation mechanisms of thermal and microwave are comparable.
期刊介绍:
Innovative Food Science and Emerging Technologies (IFSET) aims to provide the highest quality original contributions and few, mainly upon invitation, reviews on and highly innovative developments in food science and emerging food process technologies. The significance of the results either for the science community or for industrial R&D groups must be specified. Papers submitted must be of highest scientific quality and only those advancing current scientific knowledge and understanding or with technical relevance will be considered.