Pub Date : 2026-06-01Epub Date: 2026-02-10DOI: 10.1016/j.ifset.2026.104487
Zekang Peng , Chao Xu , Yue Zhang , Deqing Wang , An'an Zhang , Ziping Ai , Yanhong Liu
Radio frequency (RF) heating, as a promising alternative roasting method, could effectively improve peanut oil quality and degrade aflatoxins. However, during the peanut oil processing, the modification effect and mechanism on protein by RF roasting are still unclear. This study aimed to investigate the influences of RF roasting on the multi-scale structures and functional properties of peanut protein under 150 °C holding for 0 to 15 min. The results showed RF roasting did not alter the primary structure of peanut protein. With the increased holding time, the surface hydrophobicity and the shift of secondary structures from α-helix and β-sheet to β-turn and random coil were significantly enhanced. The changes in the intrinsic fluorescence spectrum and the contents of exposed free sulfhydryl and disulfide bond revealed that the tertiary structure underwent rearrangement and aggregation after roasting. Moderate RF roasting intensities (holding time of 5–10 min) significantly improved the emulsifying properties but had no significant effect on foaming properties of peanut protein. Additionally, the RF-roasted peanut proteins had superior emulsifying properties and formed emulsions with smaller particle size compared with hot-air-roasted ones. Overall, these findings provided new insights for the modification of plant proteins by RF roasting to enhance their value in food applications.
{"title":"Mechanistic insights into the modification of peanut protein by novel radio frequency roasting: Revealing multi-scale protein conformational changes","authors":"Zekang Peng , Chao Xu , Yue Zhang , Deqing Wang , An'an Zhang , Ziping Ai , Yanhong Liu","doi":"10.1016/j.ifset.2026.104487","DOIUrl":"10.1016/j.ifset.2026.104487","url":null,"abstract":"<div><div>Radio frequency (RF) heating, as a promising alternative roasting method, could effectively improve peanut oil quality and degrade aflatoxins. However, during the peanut oil processing, the modification effect and mechanism on protein by RF roasting are still unclear. This study aimed to investigate the influences of RF roasting on the multi-scale structures and functional properties of peanut protein under 150 °C holding for 0 to 15 min. The results showed RF roasting did not alter the primary structure of peanut protein. With the increased holding time, the surface hydrophobicity and the shift of secondary structures from α-helix and β-sheet to β-turn and random coil were significantly enhanced. The changes in the intrinsic fluorescence spectrum and the contents of exposed free sulfhydryl and disulfide bond revealed that the tertiary structure underwent rearrangement and aggregation after roasting. Moderate RF roasting intensities (holding time of 5–10 min) significantly improved the emulsifying properties but had no significant effect on foaming properties of peanut protein. Additionally, the RF-roasted peanut proteins had superior emulsifying properties and formed emulsions with smaller particle size compared with hot-air-roasted ones. Overall, these findings provided new insights for the modification of plant proteins by RF roasting to enhance their value in food applications.</div></div>","PeriodicalId":329,"journal":{"name":"Innovative Food Science & Emerging Technologies","volume":"110 ","pages":"Article 104487"},"PeriodicalIF":6.8,"publicationDate":"2026-06-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"146187615","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":1,"RegionCategory":"农林科学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
Dehulling is an essential step in the post-harvest processing of quinoa. To address the limitations of conventional dehulling methods, including low processing efficiency, severe grain damage, nutrient loss, and environmental pollution, this study developed a novel ultrasound-assisted wet dehulling (UWD) method as an effective alternative. The feasibility of UWD for quinoa dehulling was investigated by examining the effects of processing time (20–60 min), processing temperature (20–60 °C), and liquid-to-material ratio (3–11 mL/g) on dehulling performance. The results indicated that the optimal processing conditions were 38 min, 40 °C, and 7.2 mL/g, achieving a dehulling rate of 84.74% with a grain damage rate of only 1.22%. Comparative studies between optimized UWD and conventional dry dehulling (DD), wet dehulling (WD), and mixed dehulling (MD) demonstrated that UWD achieved a better balance between dehulling efficiency and grain integrity, with a dehulling rate second only to MD (87.21%) and a damage rate slightly higher than WD (0.93%). For physicochemical characteristics, UWD exhibited the most favorable overall performance, with loss rates of protein, fat, starch, and dietary fiber ranging from 5.31% to 23.76%. The loss rates of macro-elements (K, Mg, P, Ca) and micro-elements (Fe, Zn, Mn, Cu, Co) ranged from 9.58% to 39.87%. Additionally, UWD-treated grains exhibited a cleaner and brighter appearance (L* = 77.12, a* = 4.36, b* = 20.75). Principal component analysis ranked the overall scores as: UWD > MD > WD > DD. The research results provide theoretical basis and technical support for new quinoa dehulling processing technology.
{"title":"Optimization of ultrasound-assisted dehulling for quinoa and comparison with conventional methods: Dehulling performance and physicochemical characterization","authors":"Hongbin Bai, Yingsi Wu, Fei Liu, Dezheng Xuan, Xuan Zhao, Wenxue Dong, Xiang Kong, Anbin Zhang","doi":"10.1016/j.ifset.2026.104475","DOIUrl":"10.1016/j.ifset.2026.104475","url":null,"abstract":"<div><div>Dehulling is an essential step in the post-harvest processing of quinoa. To address the limitations of conventional dehulling methods, including low processing efficiency, severe grain damage, nutrient loss, and environmental pollution, this study developed a novel ultrasound-assisted wet dehulling (UWD) method as an effective alternative. The feasibility of UWD for quinoa dehulling was investigated by examining the effects of processing time (20–60 min), processing temperature (20–60 °C), and liquid-to-material ratio (3–11 mL/g) on dehulling performance. The results indicated that the optimal processing conditions were 38 min, 40 °C, and 7.2 mL/g, achieving a dehulling rate of 84.74% with a grain damage rate of only 1.22%. Comparative studies between optimized UWD and conventional dry dehulling (DD), wet dehulling (WD), and mixed dehulling (MD) demonstrated that UWD achieved a better balance between dehulling efficiency and grain integrity, with a dehulling rate second only to MD (87.21%) and a damage rate slightly higher than WD (0.93%). For physicochemical characteristics, UWD exhibited the most favorable overall performance, with loss rates of protein, fat, starch, and dietary fiber ranging from 5.31% to 23.76%. The loss rates of macro-elements (K, Mg, P, Ca) and micro-elements (Fe, Zn, Mn, Cu, Co) ranged from 9.58% to 39.87%. Additionally, UWD-treated grains exhibited a cleaner and brighter appearance (L* = 77.12, a* = 4.36, b* = 20.75). Principal component analysis ranked the overall scores as: UWD > MD > WD > DD. The research results provide theoretical basis and technical support for new quinoa dehulling processing technology.</div></div>","PeriodicalId":329,"journal":{"name":"Innovative Food Science & Emerging Technologies","volume":"110 ","pages":"Article 104475"},"PeriodicalIF":6.8,"publicationDate":"2026-06-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"146154166","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":1,"RegionCategory":"农林科学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
Pub Date : 2026-05-01Epub Date: 2026-01-16DOI: 10.1016/j.ifset.2026.104456
Enrique Martinez-Martinez, Jorge Welti-Chanes, J. Antonio Torres
The microbial preservation performance of a residential refrigerator was evaluated using a Refrigeration Preservation Indicator (RPI) based on the square root model, a frequently used secondary predictive microbiology model, and time-temperature data collected for whole milk, panela cheese and chicken breast meat. RPI values <1, ∼1, and > 1 indicating excellent, acceptable, and poor preservation performance, respectively, were used to assess effects of product placement (door/body shelves, and drawer independently controlled at 0 °C), setting (2/4/5 °C), ambient temperature (21.1 °C [LT] and 32.2 °C[HT]), and load (24.9 kg regular [RL] and 39 kg high load [HL]). Lowering the temperature setting significantly improved RPI values for all product/placement locations. While poor performance was observed at 5 °C with RPI values reaching 3.74 for milk and 1.38 for cheese, for the 2 °C setting, the corresponding values were 1.56 and 1.00. Chicken breast stored in the closed drawer with independent temperature control consistently achieved excellent performance with values ranging 0.42–1.16 across treatments. Under HL conditions, the temperature increase caused by door openings was buffered, but temperature recovery exceeded 30 h. Lowering the temperature setting to 2 °C reduced it to 9.4 h. Product exposure to ambient temperature emulating consumer product use increased RPI variability, as illustrated by one-sided 95% confidence intervals. Finally, refrigerator design improvements were identified to mitigate the lowering of food preservation performance of residential refrigerators caused by door openings and high ambient temperatures. Finally, RPI values can be used to assess the preservation performance of any cold chain component.
{"title":"Microbial preservation performance evaluation in residential refrigerators: A multiple product, refrigerator placement and temperature setting case study","authors":"Enrique Martinez-Martinez, Jorge Welti-Chanes, J. Antonio Torres","doi":"10.1016/j.ifset.2026.104456","DOIUrl":"10.1016/j.ifset.2026.104456","url":null,"abstract":"<div><div>The microbial preservation performance of a residential refrigerator was evaluated using a Refrigeration Preservation Indicator (RPI) based on the square root model, a frequently used secondary predictive microbiology model, and time-temperature data collected for whole milk, panela cheese and chicken breast meat. RPI values <1, ∼1, and > 1 indicating excellent, acceptable, and poor preservation performance, respectively, were used to assess effects of product placement (door/body shelves, and drawer independently controlled at 0 °C), setting (2/4/5 °C), ambient temperature (21.1 °C [LT] and 32.2 °C[HT]), and load (24.9 kg regular [RL] and 39 kg high load [HL]). Lowering the temperature setting significantly improved RPI values for all product/placement locations. While poor performance was observed at 5 °C with RPI values reaching 3.74 for milk and 1.38 for cheese, for the 2 °C setting, the corresponding values were 1.56 and 1.00. Chicken breast stored in the closed drawer with independent temperature control consistently achieved excellent performance with values ranging 0.42–1.16 across treatments. Under HL conditions, the temperature increase caused by door openings was buffered, but temperature recovery exceeded 30 h. Lowering the temperature setting to 2 °C reduced it to 9.4 h. Product exposure to ambient temperature emulating consumer product use increased RPI variability, as illustrated by one-sided 95% confidence intervals. Finally, refrigerator design improvements were identified to mitigate the lowering of food preservation performance of residential refrigerators caused by door openings and high ambient temperatures. Finally, RPI values can be used to assess the preservation performance of any cold chain component.</div></div>","PeriodicalId":329,"journal":{"name":"Innovative Food Science & Emerging Technologies","volume":"109 ","pages":"Article 104456"},"PeriodicalIF":6.8,"publicationDate":"2026-05-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"146073665","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":1,"RegionCategory":"农林科学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
Pub Date : 2026-05-01Epub Date: 2026-02-08DOI: 10.1016/j.ifset.2026.104483
Minghui Zhao , Jinghong Li , Sibing Li , Xiaohan Zhang , Hanyu Li , Huajiang Zhang
To mitigate post-harvest losses in field-grown fruits and thus contribute to global efforts against malnutrition and hunger, this study presents an edible coating inspired by the phase separation of ovalbumin (OVA) and chitosan (CS) complexes, resulting in distinct inner and outer functional layers. We demonstrate that the phase behavior of the OVA-CS complexes is modulated by resveratrol (RES). Specifically, RES modifies protein conformation and intermolecular interactions by enhancing hydrophobic forces and reorganizing hydrogen bonds, driving the self-assembly of a Janus-type coating during drying. Optimized at pH 5.6, the OVA-CS-RES (OCR) coating exhibits superior barrier properties, achieving the lowest water vapor permeability and oxygen permeability. Anisotropic wettability was confirmed by contact angle measurements and atomic force microscopy, with the maximum contact angle difference between the inner and outer surfaces reaching 57.2°. Scanning electron microscopy and confocal laser scanning microscopy further verified uniform coverage and effective sealing of epidermal pores on fruit surfaces. Application experiments showed that the OCR coating significantly delayed fruit oxidation, reduced weight loss, preserved texture and ascorbic acid content, and maintained aroma integrity throughout 40 days of storage. Overall, this study introduces a scalable, single-step coating strategy leveraging self-assembled functional Janus coatings to significantly extend fruit shelf life.
{"title":"Phase separation behavior of ovalbumin-chitosan: Application for the development of Janus-type fruit preservation coatings","authors":"Minghui Zhao , Jinghong Li , Sibing Li , Xiaohan Zhang , Hanyu Li , Huajiang Zhang","doi":"10.1016/j.ifset.2026.104483","DOIUrl":"10.1016/j.ifset.2026.104483","url":null,"abstract":"<div><div>To mitigate post-harvest losses in field-grown fruits and thus contribute to global efforts against malnutrition and hunger, this study presents an edible coating inspired by the phase separation of ovalbumin (OVA) and chitosan (CS) complexes, resulting in distinct inner and outer functional layers. We demonstrate that the phase behavior of the OVA-CS complexes is modulated by resveratrol (RES). Specifically, RES modifies protein conformation and intermolecular interactions by enhancing hydrophobic forces and reorganizing hydrogen bonds, driving the self-assembly of a Janus-type coating during drying. Optimized at pH 5.6, the OVA-CS-RES (OCR) coating exhibits superior barrier properties, achieving the lowest water vapor permeability and oxygen permeability. Anisotropic wettability was confirmed by contact angle measurements and atomic force microscopy, with the maximum contact angle difference between the inner and outer surfaces reaching 57.2°. Scanning electron microscopy and confocal laser scanning microscopy further verified uniform coverage and effective sealing of epidermal pores on fruit surfaces. Application experiments showed that the OCR coating significantly delayed fruit oxidation, reduced weight loss, preserved texture and ascorbic acid content, and maintained aroma integrity throughout 40 days of storage. Overall, this study introduces a scalable, single-step coating strategy leveraging self-assembled functional Janus coatings to significantly extend fruit shelf life.</div></div>","PeriodicalId":329,"journal":{"name":"Innovative Food Science & Emerging Technologies","volume":"109 ","pages":"Article 104483"},"PeriodicalIF":6.8,"publicationDate":"2026-05-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"146184823","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":1,"RegionCategory":"农林科学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
Pub Date : 2026-05-01Epub Date: 2026-01-06DOI: 10.1016/j.ifset.2026.104447
Shiqi Li , Xingnan Wang , Junjun Wang , Zhouli Wang
To enhance the colorimetric and antibacterial performance of polysaccharide-based packaging, a novel pigment-emulsion system was innovatively designed by incorporating high-stability Pickering emulsions and mixed natural dyes for freshness preservation and real-time monitoring. Specifically, anthocyanin (Ant) and curcumin (Cur) were incorporated konjac glucomannan (Kgm) matrix to expanded pH response range. Then, optimized gelatin (Gel)-cellulose nanocrystals (CNC) complexes (G-CNC7) (pH = 7) were prepared through pH-induced electrostatic interactions through eco-friendly process. Subsequently, thyme essential oil (TEO) Pickering emulsion (GCTEO) was successfully prepared and added to construct high-stability pigment/emulsion film-forming system. The antibacterial and indicative film (Kgm-Ant/Cur-GCTEO) was fabricated. The results showed that GCTEO Pickering emulsion, loaded with TEO, synergized with the inherent functional properties of Ant/Cur, effectively enhanced antibacterial and antioxidant activities. This was verified in the preservation of yellow cherry tomatoes, extending their shelf life quality and freshness. The bactericidal rates of Kgm-Ant/Cur-GCTEO against S. aureus and E. coli reached 97.32 % and 78.36 %, respectively. Furthermore, the pigment-emulsion system improved the stability and rapid responsiveness of the color indicator, expanding its pH response range for effective monitoring of yogurt and seafood freshness throughout various acid-base environments. Moreover, composite films exhibited excellent mechanical properties, light and water vapor/oxygen barrier performance, thermal stability, and enhanced hydrophobicity. This study innovatively developed a stable Pickering emulsion system combined with dual natural pigments, which represents an innovative strategy for preservation and freshness maintenance.
为了提高多糖基包装的比色性能和抗菌性能,采用高稳定性皮克林乳剂和混合天然染料,创新设计了一种新型的色素-乳液体系,用于保鲜和实时监测。在魔芋葡甘露聚糖(Kgm)基质中加入花青素(Ant)和姜黄素(Cur),扩大pH响应范围。然后,通过生态友好的工艺,通过pH诱导静电相互作用制备了优化后的明胶(Gel)-纤维素纳米晶体(CNC)配合物(G-CNC7) (pH = 7)。随后,成功制备了百里香精油皮克林乳液(GCTEO),并加入其中构建了高稳定性的颜料/乳液成膜体系。制备了抗菌指示膜(Kgm-Ant/ cu - gcteo)。结果表明,负载TEO的GCTEO Pickering乳剂与Ant/Cur固有的功能特性协同作用,有效增强了其抗菌和抗氧化活性。这在保存黄色圣女果中得到了证实,延长了它们的保质期、质量和新鲜度。Kgm-Ant/Cur-GCTEO对金黄色葡萄球菌和大肠杆菌的抑菌率分别为97.32%和78.36%。此外,颜料-乳液体系提高了颜色指示剂的稳定性和快速响应性,扩大了其pH响应范围,可在各种酸碱环境下有效监测酸奶和海鲜的新鲜度。此外,复合膜具有优异的力学性能、光和水汽/氧阻隔性能、热稳定性和增强的疏水性。本研究创新性地开发了一种结合双天然色素的稳定皮克林乳液体系,代表了一种创新的保鲜策略。
{"title":"Dual-functional smart konjac glucomannan-based packaging incorporating gelatin-cellulose nanocrystal-stabilized emulsions and synergistic anthocyanin-curcumin dyes","authors":"Shiqi Li , Xingnan Wang , Junjun Wang , Zhouli Wang","doi":"10.1016/j.ifset.2026.104447","DOIUrl":"10.1016/j.ifset.2026.104447","url":null,"abstract":"<div><div>To enhance the colorimetric and antibacterial performance of polysaccharide-based packaging, a novel pigment-emulsion system was innovatively designed by incorporating high-stability Pickering emulsions and mixed natural dyes for freshness preservation and real-time monitoring. Specifically, anthocyanin (Ant) and curcumin (Cur) were incorporated konjac glucomannan (Kgm) matrix to expanded pH response range. Then, optimized gelatin (Gel)-cellulose nanocrystals (CNC) complexes (G-CNC7) (pH = 7) were prepared through pH-induced electrostatic interactions through eco-friendly process. Subsequently, thyme essential oil (TEO) Pickering emulsion (GCTEO) was successfully prepared and added to construct high-stability pigment/emulsion film-forming system. The antibacterial and indicative film (Kgm-Ant/Cur-GCTEO) was fabricated. The results showed that GCTEO Pickering emulsion, loaded with TEO, synergized with the inherent functional properties of Ant/Cur, effectively enhanced antibacterial and antioxidant activities. This was verified in the preservation of yellow cherry tomatoes, extending their shelf life quality and freshness. The bactericidal rates of Kgm-Ant/Cur-GCTEO against <em>S. aureus</em> and <em>E. coli</em> reached 97.32 % and 78.36 %, respectively. Furthermore, the pigment-emulsion system improved the stability and rapid responsiveness of the color indicator, expanding its pH response range for effective monitoring of yogurt and seafood freshness throughout various acid-base environments. Moreover, composite films exhibited excellent mechanical properties, light and water vapor/oxygen barrier performance, thermal stability, and enhanced hydrophobicity. This study innovatively developed a stable Pickering emulsion system combined with dual natural pigments, which represents an innovative strategy for preservation and freshness maintenance.</div></div>","PeriodicalId":329,"journal":{"name":"Innovative Food Science & Emerging Technologies","volume":"109 ","pages":"Article 104447"},"PeriodicalIF":6.8,"publicationDate":"2026-05-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"145923271","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":1,"RegionCategory":"农林科学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
Tangerine leaves were valorized as a source of natural antioxidants for protecting soybean oil against lipid oxidation. Initial Soxhlet extracts obtained using hexane (HexS), ethyl acetate (EtOAc-S), and ethanol (EtOH-S) showed that the Hex-S extract provided the highest oxidative stability, highlighting the relevance of nonpolar compounds in delaying lipid oxidation. To develop a more sustainable alternative to hexane extraction, supercritical CO2 (SC-CO2) extraction was optimized using a central composite design, assessing the effects of pressure (100, 200, and 300 bar) and temperature (40, 50, and 60 °C) on the extract's antioxidant performance. The optimal SC-CO2 extract, obtained at 273 bar and 37 °C, extended the induction period of soybean oil oxidation to 5.2 h, outperforming both the Hex-S extract (4.4 h) and the control sample (3.8 h), and resulted in a lower p-anisidine value (13.36). These activities may be attributed to extracted bioactive compounds, such as linalool, thymol, and tangeretin.
{"title":"The impact of supercritical CO₂ conditions on extracting antioxidant compounds from tangerine leaves (Citrus reticulata) for enhancing oxidative stability in soybean oil","authors":"Camilo Rodríguez-García , Monique Martins Strieder , Gloria Domínguez-Rodríguez , Michael Ernesto Moreno Caballero , Liliam Palomeque , Alejandro Cifuentes , Fabián Parada-Alfonso , Elena Ibáñez","doi":"10.1016/j.ifset.2026.104449","DOIUrl":"10.1016/j.ifset.2026.104449","url":null,"abstract":"<div><div>Tangerine leaves were valorized as a source of natural antioxidants for protecting soybean oil against lipid oxidation. Initial Soxhlet extracts obtained using hexane (Hex<img>S), ethyl acetate (EtOAc-S), and ethanol (EtOH-S) showed that the Hex-S extract provided the highest oxidative stability, highlighting the relevance of nonpolar compounds in delaying lipid oxidation. To develop a more sustainable alternative to hexane extraction, supercritical CO<sub>2</sub> (SC-CO<sub>2</sub>) extraction was optimized using a central composite design, assessing the effects of pressure (100, 200, and 300 bar) and temperature (40, 50, and 60 °C) on the extract's antioxidant performance. The optimal SC-CO<sub>2</sub> extract, obtained at 273 bar and 37 °C, extended the induction period of soybean oil oxidation to 5.2 h, outperforming both the Hex-S extract (4.4 h) and the control sample (3.8 h), and resulted in a lower p-anisidine value (13.36). These activities may be attributed to extracted bioactive compounds, such as linalool, thymol, and tangeretin.</div></div>","PeriodicalId":329,"journal":{"name":"Innovative Food Science & Emerging Technologies","volume":"109 ","pages":"Article 104449"},"PeriodicalIF":6.8,"publicationDate":"2026-05-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"145923274","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":1,"RegionCategory":"农林科学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
Microbial contamination is a major cause of spoilage and quality deterioration in aquatic products, emphasizing the need for advanced non-thermal preservation strategies. This study investigated the bactericidal efficacy and preservation potential of curcumin-mediated sono/photodynamic treatment (SPDT) under variable-frequency ultrasound using Shewanella putrefaciens and large yellow croaker (Larimichthys crocea) as model systems. SPDT demonstrated a pronounced ultrasound–light synergistic effect, where acoustic cavitation enhanced light penetration and reactive oxygen species generation, effectively overcoming the shallow action depth of conventional photodynamic processes. The triple-frequency synchronous mode (20/28/40 kHz) achieved superior deep-layer bactericidal performance, reducing the total viable count by 6.28 log CFU/mL units and reaching an inactivation rate of 99.999 %. When applied to refrigerated croaker, SPDT significantly inhibited microbial growth, delayed increases in TVB-N, MDA, and pH, and preserved color, sensory quality, and odor fingerprints, extending shelf life by 3–4 days. 16S rRNA sequencing further revealed selective inhibition of dominant spoilage genera (Pseudomonas, Brochothrix, and Shewanella), suggesting broad-spectrum antibacterial activity. Correlation analysis indicated that SPDT mitigates spoilage progression by simultaneously inhibiting microbial metabolism, protein degradation, lipid oxidation, and off-flavor generation.
Industrial relevance
This study elucidates the mechanisms underlying SPDT-assisted microbial inactivation under variable-frequency ultrasound and highlights the superiority of the triple-frequency synchronous mode. The findings provide theoretical and technical support for scaling up this green, non-thermal technology in aquatic and other perishable foods.
{"title":"Triple-frequency ultrasound enhanced sono/photodynamic preservation: Mechanistic insights into curcumin-mediated microbial inactivation and quality maintenance in large yellow croaker","authors":"Dehua Wang, Biqian Wei, Tongxin Xie, Yi Zhang, Yuxuan Chen, Weiming Yan, Xiaoming Jiang, Yong Xue, Zhaojie Li, Qingjuan Tang","doi":"10.1016/j.ifset.2025.104428","DOIUrl":"10.1016/j.ifset.2025.104428","url":null,"abstract":"<div><div>Microbial contamination is a major cause of spoilage and quality deterioration in aquatic products, emphasizing the need for advanced non-thermal preservation strategies. This study investigated the bactericidal efficacy and preservation potential of curcumin-mediated sono/photodynamic treatment (SPDT) under variable-frequency ultrasound using <em>Shewanella putrefaciens</em> and large yellow croaker (<em>Larimichthys crocea</em>) as model systems. SPDT demonstrated a pronounced ultrasound–light synergistic effect, where acoustic cavitation enhanced light penetration and reactive oxygen species generation, effectively overcoming the shallow action depth of conventional photodynamic processes. The triple-frequency synchronous mode (20/28/40 kHz) achieved superior deep-layer bactericidal performance, reducing the total viable count by 6.28 log CFU/mL units and reaching an inactivation rate of 99.999 %. When applied to refrigerated croaker, SPDT significantly inhibited microbial growth, delayed increases in TVB-N, MDA, and pH, and preserved color, sensory quality, and odor fingerprints, extending shelf life by 3–4 days. 16S rRNA sequencing further revealed selective inhibition of dominant spoilage genera (<em>Pseudomonas</em>, <em>Brochothrix</em>, and <em>Shewanella</em>), suggesting broad-spectrum antibacterial activity. Correlation analysis indicated that SPDT mitigates spoilage progression by simultaneously inhibiting microbial metabolism, protein degradation, lipid oxidation, and off-flavor generation.</div></div><div><h3>Industrial relevance</h3><div>This study elucidates the mechanisms underlying SPDT-assisted microbial inactivation under variable-frequency ultrasound and highlights the superiority of the triple-frequency synchronous mode. The findings provide theoretical and technical support for scaling up this green, non-thermal technology in aquatic and other perishable foods.</div></div>","PeriodicalId":329,"journal":{"name":"Innovative Food Science & Emerging Technologies","volume":"109 ","pages":"Article 104428"},"PeriodicalIF":6.8,"publicationDate":"2026-05-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"145923275","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":1,"RegionCategory":"农林科学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
Pub Date : 2026-05-01Epub Date: 2026-01-27DOI: 10.1016/j.ifset.2026.104470
Muhammad Usama , Zaib Ali , Aashir Iqbal , Macmanus C. Ndukwu
A new method to control the surface temperature of biomass during Intermittent Microwave Drying (IMWD) is proposed in this study. The feedback-based drying method involves rewetting the food surface during drying to maintain its temperature below a certain threshold. The surface of potato slices was artificially rewetted during IMWD at different threshold temperatures for experimental validation. Containing the temperature at lower levels improved the cosmetic and nutritional quality of food. It was found that artificially rewetting the surface of potato slices when their temperature exceeded 60 °C reduced the average surface temperature by 16%. While it increased both drying time and specific energy consumption by 40%, the dried potato retained 14.2% more Vitamin C and 13.4% better color characteristics compared to the control sample. The textural quality was also improved. It was found that slices that were maintained below a lower surface temperature showed a 52.2% higher increase in hardness compared to the control sample, and they retained 43.1% more cohesiveness, resulting in harder and more mechanically stable dried slices. The study revealed that this method of drying resulted in a significantly improved nutritional, cosmetic, and textural quality of the product.
{"title":"Controlling surface temperature in Intermittent Microwave Drying of potato slices using evaporative cooling: An experimental study","authors":"Muhammad Usama , Zaib Ali , Aashir Iqbal , Macmanus C. Ndukwu","doi":"10.1016/j.ifset.2026.104470","DOIUrl":"10.1016/j.ifset.2026.104470","url":null,"abstract":"<div><div>A new method to control the surface temperature of biomass during Intermittent Microwave Drying (IMWD) is proposed in this study. The feedback-based drying method involves rewetting the food surface during drying to maintain its temperature below a certain threshold. The surface of potato slices was artificially rewetted during IMWD at different threshold temperatures for experimental validation. Containing the temperature at lower levels improved the cosmetic and nutritional quality of food. It was found that artificially rewetting the surface of potato slices when their temperature exceeded 60 °C reduced the average surface temperature by 16%. While it increased both drying time and specific energy consumption by 40%, the dried potato retained 14.2% more Vitamin C and 13.4% better color characteristics compared to the control sample. The textural quality was also improved. It was found that slices that were maintained below a lower surface temperature showed a 52.2% higher increase in hardness compared to the control sample, and they retained 43.1% more cohesiveness, resulting in harder and more mechanically stable dried slices. The study revealed that this method of drying resulted in a significantly improved nutritional, cosmetic, and textural quality of the product.</div></div>","PeriodicalId":329,"journal":{"name":"Innovative Food Science & Emerging Technologies","volume":"109 ","pages":"Article 104470"},"PeriodicalIF":6.8,"publicationDate":"2026-05-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"146074178","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":1,"RegionCategory":"农林科学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
Pub Date : 2026-05-01Epub Date: 2026-02-05DOI: 10.1016/j.ifset.2026.104479
Marry Albright Johnson, Mahesh Kumar, Vandna Sharma, Sveta Thakur
This study examined the effects of different spectral LED (light-emitting diode) light treatments on growth performance, physiological responses, and underlying ionomic variations in 6-day-old sprouts of two underutilized western Himalayan buckwheat species, namely Fagopyrum esculentum Moench (common buckwheat) and F. tataricum Gaertn. (Tartary buckwheat). Both species responded differentially to spectral modulation, exhibiting variation in overall growth and elemental distribution. The highest biomass accumulation was predominantly reported in common buckwheat, whereas Tartary buckwheat showed a more pronounced ionomic shift across different spectral treatments. Blue light significantly enhanced elemental concentrations in both species. Specifically, it improved Na, Mn, Mg, Co, and Cu concentrations in Tartary buckwheat, whereas B, Fe, and Cu concentrations in common buckwheat. Multivariate analysis, including hierarchical clustering and principal component analysis, confirmed distinct ionomic separation patterns between treated and control groups. A linear association was also revealed among several elemental pairs, indicating coordinated nutrient regulation in response to spectral light modulation. Blue light further improved APX, CAT, Vitamin C, and total protein levels in both species, whereas red light stimulated SOD activity, demonstrating wavelength-specific regulation of enzymatic antioxidative potential. In contrast, the 70Blue: 30Red spectral combination increased the total sugar content while simultaneously promoting the accumulation of photosynthetic and photoprotective pigments. The study explicitly highlights the importance of species-specific lighting strategies tailored to the targeted enhancement of biomass, pigments, antioxidants, and ionomic profile in a controlled environment. Additionally, the findings offer a promising approach for advancing the functional and nutraceutical quality of Himalayan buckwheat sprouts, ultimately improving their consumer acceptance and commercial potential.
{"title":"Spectral light regulation enhances sprouting, physiological efficiency, and ionomic homeostasis in Western Himalayan buckwheat","authors":"Marry Albright Johnson, Mahesh Kumar, Vandna Sharma, Sveta Thakur","doi":"10.1016/j.ifset.2026.104479","DOIUrl":"10.1016/j.ifset.2026.104479","url":null,"abstract":"<div><div>This study examined the effects of different spectral LED (light-emitting diode) light treatments on growth performance, physiological responses, and underlying ionomic variations in 6-day-old sprouts of two underutilized western Himalayan buckwheat species, namely <em>Fagopyrum esculentum</em> Moench (common buckwheat) and <em>F. tataricum</em> Gaertn. (Tartary buckwheat). Both species responded differentially to spectral modulation, exhibiting variation in overall growth and elemental distribution. The highest biomass accumulation was predominantly reported in common buckwheat, whereas Tartary buckwheat showed a more pronounced ionomic shift across different spectral treatments. Blue light significantly enhanced elemental concentrations in both species. Specifically, it improved Na, Mn, Mg, Co, and Cu concentrations in Tartary buckwheat, whereas B, Fe, and Cu concentrations in common buckwheat. Multivariate analysis, including hierarchical clustering and principal component analysis, confirmed distinct ionomic separation patterns between treated and control groups. A linear association was also revealed among several elemental pairs, indicating coordinated nutrient regulation in response to spectral light modulation. Blue light further improved APX, CAT, Vitamin C, and total protein levels in both species, whereas red light stimulated SOD activity, demonstrating wavelength-specific regulation of enzymatic antioxidative potential. In contrast, the 70Blue: 30Red spectral combination increased the total sugar content while simultaneously promoting the accumulation of photosynthetic and photoprotective pigments. The study explicitly highlights the importance of species-specific lighting strategies tailored to the targeted enhancement of biomass, pigments, antioxidants, and ionomic profile in a controlled environment. Additionally, the findings offer a promising approach for advancing the functional and nutraceutical quality of Himalayan buckwheat sprouts, ultimately improving their consumer acceptance and commercial potential.</div></div>","PeriodicalId":329,"journal":{"name":"Innovative Food Science & Emerging Technologies","volume":"109 ","pages":"Article 104479"},"PeriodicalIF":6.8,"publicationDate":"2026-05-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"146184822","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":1,"RegionCategory":"农林科学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
Pub Date : 2026-05-01Epub Date: 2026-01-30DOI: 10.1016/j.ifset.2026.104471
Jae-Wan Ryu , Dong-Hyun Kang
This study aimed to develop and optimize a novel combined plasma–ultrasound system for the effective inactivation of foodborne pathogens in water and on the surface of fresh produce. The bactericidal efficacy of various treatment modes, including plasma alone, ultrasound alone, simultaneous application, and sequential application, was evaluated. Among these, sequential treatment where ultrasound was applied prior to plasma (Seq (UP)) demonstrated the highest antimicrobial effectiveness. Under optimized plasma–ultrasound conditions, this treatment significantly increased the leakage of intracellular proteins and DNA, indicating severe membrane damage. Furthermore, quantitative analysis showed that the Seq (UP) condition produced the highest ROS signals, with hydroxyl radical fluorescence reaching 564.6 a.u. Scavenger assays confirmed that these ROS played a critical role in microbial inactivation by damaging cellular components such as membranes, proteins, and DNA. The system was also tested on fresh produce (cherry tomatoes), where the Seq (UP) condition maintained superior microbicidal efficacy and ROS generation. These findings suggested that ultrasound-induced cavitation enhanced membrane permeability, allowing more effective penetration of plasma-generated reactive species. The study demonstrated that the optimized Seq (UP) plasma–ultrasound system provided a synergistic, chemical-free, and environmentally friendly method for microbial decontamination. This system showed strong potential for application in the food industry, particularly for improving the safety of fresh produce without relying on conventional chemical sanitizers. Future work will focus on the system's scalability and practical integration into food processing environments.
{"title":"Optimization of a combined plasma–ultrasound system for synergistic microbial inactivation and ROS-mediated mechanism on fresh produce","authors":"Jae-Wan Ryu , Dong-Hyun Kang","doi":"10.1016/j.ifset.2026.104471","DOIUrl":"10.1016/j.ifset.2026.104471","url":null,"abstract":"<div><div>This study aimed to develop and optimize a novel combined plasma–ultrasound system for the effective inactivation of foodborne pathogens in water and on the surface of fresh produce. The bactericidal efficacy of various treatment modes, including plasma alone, ultrasound alone, simultaneous application, and sequential application, was evaluated. Among these, sequential treatment where ultrasound was applied prior to plasma (Seq (U<img>P)) demonstrated the highest antimicrobial effectiveness. Under optimized plasma–ultrasound conditions, this treatment significantly increased the leakage of intracellular proteins and DNA, indicating severe membrane damage. Furthermore, quantitative analysis showed that the Seq (U<img>P) condition produced the highest ROS signals, with hydroxyl radical fluorescence reaching 564.6 a.u. Scavenger assays confirmed that these ROS played a critical role in microbial inactivation by damaging cellular components such as membranes, proteins, and DNA. The system was also tested on fresh produce (cherry tomatoes), where the Seq (U<img>P) condition maintained superior microbicidal efficacy and ROS generation. These findings suggested that ultrasound-induced cavitation enhanced membrane permeability, allowing more effective penetration of plasma-generated reactive species. The study demonstrated that the optimized Seq (U<img>P) plasma–ultrasound system provided a synergistic, chemical-free, and environmentally friendly method for microbial decontamination. This system showed strong potential for application in the food industry, particularly for improving the safety of fresh produce without relying on conventional chemical sanitizers. Future work will focus on the system's scalability and practical integration into food processing environments.</div></div>","PeriodicalId":329,"journal":{"name":"Innovative Food Science & Emerging Technologies","volume":"109 ","pages":"Article 104471"},"PeriodicalIF":6.8,"publicationDate":"2026-05-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"146184905","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":1,"RegionCategory":"农林科学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}