{"title":"从患病猫狗中分离出的对第三代头孢菌素耐药的大肠埃希菌菌株的基因组特征:日本兽医抗菌药耐药性监测报告","authors":"Yukari Hiraoka (Furuya), Hitoshi Abo, Mari Matsuda, Saki Harada, Mio Kumakawa, Takahiro Shirakawa, Manao Ozawa, Michiko Kawanishi, Hideto Sekiguchi, Yoko Shimazaki","doi":"10.1016/j.vetmic.2024.110220","DOIUrl":null,"url":null,"abstract":"<div><p>This study investigates the genomic characteristics of canine and feline cefotaxime (CTX, a third-generation cephalosporin)-resistant <em>Escherichia coli</em> using the JVARM, Japanese Veterinary Antimicrobial Resistance Monitoring System, a nationwide monitoring.</p><p>In this study, whole-genome sequencing (WGS) was performed on 51 canine and 45 feline CTX-resistant <em>E. coli</em> isolates, with certain isolates subjected to pulsed-field gel electrophoresis with S1 nuclease for plasmid–chromosome separation.</p><p>The most common <em>bla</em><sub>CTX-M</sub> genes were <em>bla</em><sub>CTX-M-27</sub> (dogs: 11/51 [21.6 %]; cat: 10/45 [22.2 %]), followed by <em>bla</em><sub>CTX-M-14</sub> (dogs: 10/51 [19.6 %]; cats: 10/45 [22.2 %]), and <em>bla</em><sub>CTX-M-15</sub> (dogs: 9/51 [17.6 %]; cats: 5/45 [11.1 %]). Besides β-lactamase genes, all isolates harbored <em>mdf</em>(A), a multidrug efflux pump, with resistance genes for aminoglycosides, sulfonamides, trimethoprims, macrolides and tetracyclines. None of the isolates had carbapenemase genes, such as <em>bla</em><sub>OXA-48</sub>, <em>bla</em><sub>NDM</sub>, and <em>bla</em><sub>IMP</sub>, whereas most of the isolates showed double mutations in <em>gyrA</em> and <em>parC</em>, which affected quinolone resistance. For the isolates separately analyzed for plasmid and chromosomal DNA via WGS, the majority of CTX-M genes were present on the plasmids. Some plasmids also harbored the same combination of resistance genes and plasmid replicon type, although they differed from isolates derived from different areas of Japan. The predominant plasmids were <em>bla</em><sub>CTX-M</sub>-<sub>27,</sub> <em>aadA5, aph(6)-Id, aph(3”)-Ib, sul1, sul2, tet</em>(A)<em>, dfrA17</em>, and <em>mph</em>(A) on IncF. The predominant combination of ST131, O25:H4, and B2 isolates comprised the largest cluster in the minimum spanning tree and the ST131 <em>E. coli</em> harboring <em>bla</em><sub>CTX-M-27</sub> from human in Japan was closely related to these isolates.</p><p>The results indicated that CTX-resistant canine and feline <em>E. coli</em> harbored multiple plasmids carrying the same combination of resistance genes and emphasizes the need to prevent the spread.</p></div><div><h3>Data Availability</h3><p>All raw short-read sequence data have been deposited in the DNA Data Bank of Japan. (DRR Run No, DRR335726–335821).</p></div>","PeriodicalId":23551,"journal":{"name":"Veterinary microbiology","volume":"298 ","pages":"Article 110220"},"PeriodicalIF":2.4000,"publicationDate":"2024-08-24","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://www.sciencedirect.com/science/article/pii/S0378113524002426/pdfft?md5=caa688667aa8ce50c9fcc5b0b32cf762&pid=1-s2.0-S0378113524002426-main.pdf","citationCount":"0","resultStr":"{\"title\":\"Genomic characterization of third-generation cephalosporin-resistant Escherichia coli strains isolated from diseased dogs and cats: Report from Japanese Veterinary Antimicrobial Resistance Monitoring\",\"authors\":\"Yukari Hiraoka (Furuya), Hitoshi Abo, Mari Matsuda, Saki Harada, Mio Kumakawa, Takahiro Shirakawa, Manao Ozawa, Michiko Kawanishi, Hideto Sekiguchi, Yoko Shimazaki\",\"doi\":\"10.1016/j.vetmic.2024.110220\",\"DOIUrl\":null,\"url\":null,\"abstract\":\"<div><p>This study investigates the genomic characteristics of canine and feline cefotaxime (CTX, a third-generation cephalosporin)-resistant <em>Escherichia coli</em> using the JVARM, Japanese Veterinary Antimicrobial Resistance Monitoring System, a nationwide monitoring.</p><p>In this study, whole-genome sequencing (WGS) was performed on 51 canine and 45 feline CTX-resistant <em>E. coli</em> isolates, with certain isolates subjected to pulsed-field gel electrophoresis with S1 nuclease for plasmid–chromosome separation.</p><p>The most common <em>bla</em><sub>CTX-M</sub> genes were <em>bla</em><sub>CTX-M-27</sub> (dogs: 11/51 [21.6 %]; cat: 10/45 [22.2 %]), followed by <em>bla</em><sub>CTX-M-14</sub> (dogs: 10/51 [19.6 %]; cats: 10/45 [22.2 %]), and <em>bla</em><sub>CTX-M-15</sub> (dogs: 9/51 [17.6 %]; cats: 5/45 [11.1 %]). Besides β-lactamase genes, all isolates harbored <em>mdf</em>(A), a multidrug efflux pump, with resistance genes for aminoglycosides, sulfonamides, trimethoprims, macrolides and tetracyclines. None of the isolates had carbapenemase genes, such as <em>bla</em><sub>OXA-48</sub>, <em>bla</em><sub>NDM</sub>, and <em>bla</em><sub>IMP</sub>, whereas most of the isolates showed double mutations in <em>gyrA</em> and <em>parC</em>, which affected quinolone resistance. For the isolates separately analyzed for plasmid and chromosomal DNA via WGS, the majority of CTX-M genes were present on the plasmids. Some plasmids also harbored the same combination of resistance genes and plasmid replicon type, although they differed from isolates derived from different areas of Japan. The predominant plasmids were <em>bla</em><sub>CTX-M</sub>-<sub>27,</sub> <em>aadA5, aph(6)-Id, aph(3”)-Ib, sul1, sul2, tet</em>(A)<em>, dfrA17</em>, and <em>mph</em>(A) on IncF. The predominant combination of ST131, O25:H4, and B2 isolates comprised the largest cluster in the minimum spanning tree and the ST131 <em>E. coli</em> harboring <em>bla</em><sub>CTX-M-27</sub> from human in Japan was closely related to these isolates.</p><p>The results indicated that CTX-resistant canine and feline <em>E. coli</em> harbored multiple plasmids carrying the same combination of resistance genes and emphasizes the need to prevent the spread.</p></div><div><h3>Data Availability</h3><p>All raw short-read sequence data have been deposited in the DNA Data Bank of Japan. (DRR Run No, DRR335726–335821).</p></div>\",\"PeriodicalId\":23551,\"journal\":{\"name\":\"Veterinary microbiology\",\"volume\":\"298 \",\"pages\":\"Article 110220\"},\"PeriodicalIF\":2.4000,\"publicationDate\":\"2024-08-24\",\"publicationTypes\":\"Journal Article\",\"fieldsOfStudy\":null,\"isOpenAccess\":false,\"openAccessPdf\":\"https://www.sciencedirect.com/science/article/pii/S0378113524002426/pdfft?md5=caa688667aa8ce50c9fcc5b0b32cf762&pid=1-s2.0-S0378113524002426-main.pdf\",\"citationCount\":\"0\",\"resultStr\":null,\"platform\":\"Semanticscholar\",\"paperid\":null,\"PeriodicalName\":\"Veterinary microbiology\",\"FirstCategoryId\":\"97\",\"ListUrlMain\":\"https://www.sciencedirect.com/science/article/pii/S0378113524002426\",\"RegionNum\":2,\"RegionCategory\":\"农林科学\",\"ArticlePicture\":[],\"TitleCN\":null,\"AbstractTextCN\":null,\"PMCID\":null,\"EPubDate\":\"\",\"PubModel\":\"\",\"JCR\":\"Q3\",\"JCRName\":\"MICROBIOLOGY\",\"Score\":null,\"Total\":0}","platform":"Semanticscholar","paperid":null,"PeriodicalName":"Veterinary microbiology","FirstCategoryId":"97","ListUrlMain":"https://www.sciencedirect.com/science/article/pii/S0378113524002426","RegionNum":2,"RegionCategory":"农林科学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":null,"EPubDate":"","PubModel":"","JCR":"Q3","JCRName":"MICROBIOLOGY","Score":null,"Total":0}
Genomic characterization of third-generation cephalosporin-resistant Escherichia coli strains isolated from diseased dogs and cats: Report from Japanese Veterinary Antimicrobial Resistance Monitoring
This study investigates the genomic characteristics of canine and feline cefotaxime (CTX, a third-generation cephalosporin)-resistant Escherichia coli using the JVARM, Japanese Veterinary Antimicrobial Resistance Monitoring System, a nationwide monitoring.
In this study, whole-genome sequencing (WGS) was performed on 51 canine and 45 feline CTX-resistant E. coli isolates, with certain isolates subjected to pulsed-field gel electrophoresis with S1 nuclease for plasmid–chromosome separation.
The most common blaCTX-M genes were blaCTX-M-27 (dogs: 11/51 [21.6 %]; cat: 10/45 [22.2 %]), followed by blaCTX-M-14 (dogs: 10/51 [19.6 %]; cats: 10/45 [22.2 %]), and blaCTX-M-15 (dogs: 9/51 [17.6 %]; cats: 5/45 [11.1 %]). Besides β-lactamase genes, all isolates harbored mdf(A), a multidrug efflux pump, with resistance genes for aminoglycosides, sulfonamides, trimethoprims, macrolides and tetracyclines. None of the isolates had carbapenemase genes, such as blaOXA-48, blaNDM, and blaIMP, whereas most of the isolates showed double mutations in gyrA and parC, which affected quinolone resistance. For the isolates separately analyzed for plasmid and chromosomal DNA via WGS, the majority of CTX-M genes were present on the plasmids. Some plasmids also harbored the same combination of resistance genes and plasmid replicon type, although they differed from isolates derived from different areas of Japan. The predominant plasmids were blaCTX-M-27,aadA5, aph(6)-Id, aph(3”)-Ib, sul1, sul2, tet(A), dfrA17, and mph(A) on IncF. The predominant combination of ST131, O25:H4, and B2 isolates comprised the largest cluster in the minimum spanning tree and the ST131 E. coli harboring blaCTX-M-27 from human in Japan was closely related to these isolates.
The results indicated that CTX-resistant canine and feline E. coli harbored multiple plasmids carrying the same combination of resistance genes and emphasizes the need to prevent the spread.
Data Availability
All raw short-read sequence data have been deposited in the DNA Data Bank of Japan. (DRR Run No, DRR335726–335821).
期刊介绍:
Veterinary Microbiology is concerned with microbial (bacterial, fungal, viral) diseases of domesticated vertebrate animals (livestock, companion animals, fur-bearing animals, game, poultry, fish) that supply food, other useful products or companionship. In addition, Microbial diseases of wild animals living in captivity, or as members of the feral fauna will also be considered if the infections are of interest because of their interrelation with humans (zoonoses) and/or domestic animals. Studies of antimicrobial resistance are also included, provided that the results represent a substantial advance in knowledge. Authors are strongly encouraged to read - prior to submission - the Editorials (''Scope or cope'' and ''Scope or cope II'') published previously in the journal. The Editors reserve the right to suggest submission to another journal for those papers which they feel would be more appropriate for consideration by that journal.
Original research papers of high quality and novelty on aspects of control, host response, molecular biology, pathogenesis, prevention, and treatment of microbial diseases of animals are published. Papers dealing primarily with immunology, epidemiology, molecular biology and antiviral or microbial agents will only be considered if they demonstrate a clear impact on a disease. Papers focusing solely on diagnostic techniques (such as another PCR protocol or ELISA) will not be published - focus should be on a microorganism and not on a particular technique. Papers only reporting microbial sequences, transcriptomics data, or proteomics data will not be considered unless the results represent a substantial advance in knowledge.
Drug trial papers will be considered if they have general application or significance. Papers on the identification of microorganisms will also be considered, but detailed taxonomic studies do not fall within the scope of the journal. Case reports will not be published, unless they have general application or contain novel aspects. Papers of geographically limited interest, which repeat what had been established elsewhere will not be considered. The readership of the journal is global.