低成本、可扩展的投射光片显微镜,用于对清除的组织和活体样本进行高分辨率成像

IF 26.8 1区 医学 Q1 ENGINEERING, BIOMEDICAL Nature Biomedical Engineering Pub Date : 2024-08-29 DOI:10.1038/s41551-024-01249-9
Yannan Chen, Shradha Chauhan, Cheng Gong, Hannah Dayton, Cong Xu, Estanislao Daniel De La Cruz, Yu-Young Wesley Tsai, Malika S. Datta, Gorazd B. Rosoklija, Andrew J. Dwork, J. John Mann, Maura Boldrini, Kam W. Leong, Lars E. P. Dietrich, Raju Tomer
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引用次数: 0

摘要

光片荧光显微镜(LSFM)是一种广泛用于对清除的组织和活体样本进行成像的技术。然而,高性能 LSFM 系统通常价格昂贵且不易扩展。在这里,我们介绍了一种低成本、可扩展和多功能的 LSFM 框架,并将其命名为 "投射光片显微镜"(pLSM),它具有成像性能高、设备和计算占用空间小的特点。我们通过对清除的小鼠大脑和死后病理人脑样本进行高分辨率制图,以及对从人类诱导多能干细胞中提取的大脑和血管有机体进行分子表型,证明了投射光片显微镜的能力。我们还报告了一种利用 pLSM 对空气-液体界面上稀疏标记的多层细菌胶粒生物膜的动态进行实时成像的方法。pLSM 可以使生物医学应用中的高分辨率 LSFM 更容易获得、更经济实惠、更可扩展。
本文章由计算机程序翻译,如有差异,请以英文原文为准。

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Low-cost and scalable projected light-sheet microscopy for the high-resolution imaging of cleared tissue and living samples
Light-sheet fluorescence microscopy (LSFM) is a widely used technique for imaging cleared tissue and living samples. However, high-performance LSFM systems are typically expensive and not easily scalable. Here we introduce a low-cost, scalable and versatile LSFM framework, which we named ‘projected light-sheet microscopy’ (pLSM), with high imaging performance and small device and computational footprints. We characterized the capabilities of pLSM, which repurposes readily available consumer-grade components, optimized optics, over-network control architecture and software-driven light-sheet modulation, by performing high-resolution mapping of cleared mouse brains and of post-mortem pathological human brain samples, and via the molecular phenotyping of brain and blood-vessel organoids derived from human induced pluripotent stem cells. We also report a method that leverages pLSM for the live imaging of the dynamics of sparsely labelled multi-layered bacterial pellicle biofilms at an air–liquid interface. pLSM can make high-resolution LSFM for biomedical applications more accessible, affordable and scalable. A light-sheet fluorescence microscope leveraging consumer-grade components as well as optimized optics and software facilitates the high-resolution imaging of cleared and living samples at scale with lower costs.
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来源期刊
Nature Biomedical Engineering
Nature Biomedical Engineering Medicine-Medicine (miscellaneous)
CiteScore
45.30
自引率
1.10%
发文量
138
期刊介绍: Nature Biomedical Engineering is an online-only monthly journal that was launched in January 2017. It aims to publish original research, reviews, and commentary focusing on applied biomedicine and health technology. The journal targets a diverse audience, including life scientists who are involved in developing experimental or computational systems and methods to enhance our understanding of human physiology. It also covers biomedical researchers and engineers who are engaged in designing or optimizing therapies, assays, devices, or procedures for diagnosing or treating diseases. Additionally, clinicians, who make use of research outputs to evaluate patient health or administer therapy in various clinical settings and healthcare contexts, are also part of the target audience.
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