Ava Hashempour, Nastaran Khodadad, Shokufeh Akbarinia, Farzane Ghasabi, Younes Ghasemi, Mohamad Matin Karbalaei Ali Nazar, Shahab Falahi
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引用次数: 0
摘要
在开发有望消除 HIV-1 感染的 HIV 疫苗方面取得了重大进展。我们首次从 LANL 数据库中检索到 100 种提交最多的 HIV 亚型和 CRF,并获得了 11 种 HIV 蛋白的共识序列,从而设计出适用于人类和小鼠宿主的疫苗。通过使用各种服务器和过滤器,在疫苗构建中考虑了小鼠和人类等位基因之间常见的、位于 HIV 蛋白保守结构域的高合格 B 细胞表位以及 HTL 和 CD8 + 表位。人类疫苗模型在全球的覆盖率为 90%,涵盖了不同的等位基因群体,因此可广泛使用。密码子优化和在原核和真核载体中的硅克隆保证了疫苗模型在人类和大肠杆菌宿主中的高表达。分子动力学证实了疫苗构建体与 TLR3、TLR4 和 TLR9 的稳定相互作用,从而使设计的疫苗产生大量免疫原性反应。疫苗模型能有效地针对人和小鼠的体液免疫和细胞免疫系统;然而,还需要实验验证来证实这些在硅学中的发现。
Reverse vaccinology approaches to design a potent multiepitope vaccine against the HIV whole genome: immunoinformatic, bioinformatics, and molecular dynamics approaches.
Substantial advances have been made in the development of promising HIV vaccines to eliminate HIV-1 infection. For the first time, one hundred of the most submitted HIV subtypes and CRFs were retrieved from the LANL database, and the consensus sequences of the eleven HIV proteins were obtained to design vaccines for human and mouse hosts. By using various servers and filters, highly qualified B-cell epitopes, as well as HTL and CD8 + epitopes that were common between mouse and human alleles and were also located in the conserved domains of HIV proteins, were considered in the vaccine constructs. With 90% coverage worldwide, the human vaccine model covers a diverse allelic population, making it widely available. Codon optimization and in silico cloning in prokaryotic and eukaryotic vectors guarantee high expression of the vaccine models in human and E. coli hosts. Molecular dynamics confirmed the stable interaction of the vaccine constructs with TLR3, TLR4, and TLR9, leading to a substantial immunogenic response to the designed vaccine. Vaccine models effectively target the humoral and cellular immune systems in humans and mice; however, experimental validation is needed to confirm these findings in silico.
期刊介绍:
BMC Infectious Diseases is an open access, peer-reviewed journal that considers articles on all aspects of the prevention, diagnosis and management of infectious and sexually transmitted diseases in humans, as well as related molecular genetics, pathophysiology, and epidemiology.