Anfal Kara, Chiara Massaro, Giovanni M Giammanco, Rosa Alduina, Naouel Boussoualim
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引用次数: 0
摘要
由大肠埃希菌引起的尿路感染(UTI)是一个重大的公共卫生问题,因为这些病原体具有很强的毒力和抗菌药耐药性。本研究旨在分析从阿尔及利亚尿路感染患者中分离出的尿路致病性大肠杆菌(UPEC)菌株的系统发育多样性和抗生素耐药性特征,重点关注毒力因素,如扩展β-内酰胺酶(ESBL)的产生、生物膜的形成和溶血活性。对 86 个临床亚胺培南耐药大肠杆菌分离物进行的系统发育分组显示,B2 群(48.9%)的流行率最高,其次是 E 群(22.1%)、未知群(12.8%)、A 群(8.1%)和 B1 群(4.7%),以及 I 群、D 群、I 群或 II 群(1.2%)。阿莫西林(86.04%)、替卡西林(82.55%)、哌拉西林(73.25%)、硝基呋喃妥因(84.88%)和三甲双氨-磺胺甲噁唑(51.16%)的耐药率最高。值得注意的是,69.8%的 UPEC 菌株具有多重耐药性(MDR),23.2%具有广泛耐药性(XDR)。此外,分别有 48.9%、42% 和 71% 的菌株表现出 ESBL 生产、溶血活性和弱生物膜生产。对 UPEC 菌株的持续监测和特征描述对于追踪耐药性最强、毒性最大的系统发育群的长期传播至关重要,有助于快速做出治疗感染的决定,防止出现新的耐药生物,帮助选择最有效的抗生素,减少治疗失败。
Phylogenetic Diversity, Antibiotic Resistance, and Virulence of Escherichia coli Strains from Urinary Tract Infections in Algeria.
Urinary tract infections (UTIs) caused by Escherichia coli represent a significant public health concern due to the high virulence and antimicrobial resistance exhibited by these pathogens. This study aimed to analyze the phylogenetic diversity and antibiotic resistance profiles of Uropathogenic E. coli (UPEC) strains isolated from UTI patients in Algeria, focusing on virulence factors such as extended β-lactamase (ESBL) production, biofilm formation, and hemolytic activity. Phylogenetic grouping of 86 clinical imipenem resistant E. coli isolates showed the prevalence of group B2 (48.9%), followed by groups E (22.1%), unknown (12.8%), A (8.1%), and B1 (4.7%), and Clade I, D, Clade I, or Clade II (1.2%). The highest resistance rates were observed towards amoxicillin (86.04%), ticarcillin (82.55%), piperacillin (73.25%), nitrofurantoin (84.88%), and trimethoprim-sulfamethoxazole (51.16%). Notably, 69.8% of UPEC strains were multidrug-resistant (MDR) and 23.2% were extensively drug-resistant (XDR). Additionally, 48.9%, 42%, and 71% of strains demonstrated ESBL production, hemolytic activity, and weak biofilm production, respectively. Continuous monitoring and characterization of UPEC strains are essential to track the spread of the most resistant and virulent phylogenetic groups over time, facilitating rapid therapeutic decisions to treat infections and prevent the emergence of new resistant organisms, helping choose the most effective antibiotics and reducing treatment failure.
Antibiotics-BaselPharmacology, Toxicology and Pharmaceutics-General Pharmacology, Toxicology and Pharmaceutics
CiteScore
7.30
自引率
14.60%
发文量
1547
审稿时长
11 weeks
期刊介绍:
Antibiotics (ISSN 2079-6382) is an open access, peer reviewed journal on all aspects of antibiotics. Antibiotics is a multi-disciplinary journal encompassing the general fields of biochemistry, chemistry, genetics, microbiology and pharmacology. Our aim is to encourage scientists to publish their experimental and theoretical results in as much detail as possible. Therefore, there is no restriction on the length of papers.