荧光光谱与温度功能测试在细胞内能量代谢标志物评估中的应用:测量的空间异质性和可重复性。

IF 2 3区 物理与天体物理 Q3 BIOCHEMICAL RESEARCH METHODS Journal of Biophotonics Pub Date : 2024-08-28 DOI:10.1002/jbio.202400294
Ekaterina Ryzhkova, Tatyana Morgunova, Elena Potapova, Ivan Ryzhkov, Valentin Fadeyev
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引用次数: 0

摘要

细胞呼吸辅助因子 NADH(还原型烟酰胺腺嘌呤二核苷酸)和 FAD++(氧化型黄素腺嘌呤二核苷酸)的荧光强度反映了皮肤和其他组织的能量代谢情况,可通过荧光光谱法(FS)进行活体量化。然而,生理参数的可变性在很大程度上决定了测量结果的可重复性和诊断测试的可靠性。在这项前瞻性研究中,我们评估了 51 名健康志愿者皮肤中 NADH 和 FAD++ 荧光强度测量值在基线、局部冷却(10°C)和皮肤加热(35°C)后的重现性。结果显示,在所研究的 FS 参数中,前臂皮肤中 NADH(AFNADH)荧光振幅的重现性最好。结合热功能测试评估前臂背侧的 AFNADH 是最有希望用于临床评估皮肤能量代谢的方法。
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Fluorescence Spectroscopy With Temperature Functional Tests in the Assessment of Markers of Intracellular Energy Metabolism: Spatial Heterogeneity and Reproducibility of Measurements

The fluorescence intensities of the cellular respiratory cofactors NADH (reduced nicotinamide adenine dinucleotide) and FAD++ (oxidized flavin adenine dinucleotide) reflect energy metabolism in skin and other tissues and can be quantified in vivo by fluorescence spectroscopy (FS). However, the variability of physiological parameters largely determines the reproducibility of measurement results and the reliability of the diagnostic test. In this prospective study, we evaluated the interday reproducibility of NADH and FAD++ fluorescence intensity measurements in the skin of 51 healthy volunteers assessed by the FS at baseline, after local cooling (10°C) and heating of the skin (35°C). Results showed that the fluorescence amplitude of NADH (AFNADH) in forearm skin was the most reproducible of the FS parameters studied. Assessment of AFNADH in the dorsal forearm in combination with a thermal functional test is the most promising method for clinical use for assessing energy metabolism in the skin.

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来源期刊
Journal of Biophotonics
Journal of Biophotonics 生物-生化研究方法
CiteScore
5.70
自引率
7.10%
发文量
248
审稿时长
1 months
期刊介绍: The first international journal dedicated to publishing reviews and original articles from this exciting field, the Journal of Biophotonics covers the broad range of research on interactions between light and biological material. The journal offers a platform where the physicist communicates with the biologist and where the clinical practitioner learns about the latest tools for the diagnosis of diseases. As such, the journal is highly interdisciplinary, publishing cutting edge research in the fields of life sciences, medicine, physics, chemistry, and engineering. The coverage extends from fundamental research to specific developments, while also including the latest applications.
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