利用太平洋牡蛎 β-1,3-半乳糖基转移酶对糖共轭物进行半乳糖基化

IF 2.4 3区 化学 Q3 BIOCHEMISTRY & MOLECULAR BIOLOGY Carbohydrate Research Pub Date : 2024-08-28 DOI:10.1016/j.carres.2024.109254
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引用次数: 0

摘要

太平洋牡蛎(Magallana gigas)表现出广泛的 N- 和 O-连接糖共轭物多样性,为生物技术应用提供了巨大潜力。通过基因组数据挖掘,我们发现并鉴定了一系列β-1,3-半乳糖基转移酶,它们是合成聚糖结构的关键。在克隆的十个候选基因中,有六个酶成功地在大肠杆菌中重组表达。其中四种酶在将半乳糖转移到各种受体底物的过程中表现出可测量的催化活性。值得注意的是,MgB3GalT1 的效率最高,达到了 91.2% 的转化率。这种酶能熟练地糖基化各种聚糖结构,包括核心 2 O-聚糖和几种二元、三元和四元触角复合 N-聚糖标准。质谱分析证实了 N-聚糖的成功修饰。这些发现为利用牡蛎衍生酶进行基于聚糖的治疗和分子糖工程开辟了新途径,突出了它们在合成应用和生物技术进步中的实用性。
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Galactosylation of glycoconjugates using Pacific oyster β-1,3-galactosyltransferases

The Pacific oyster (Magallana gigas) exhibits an extensive diversity of N- and O-linked glycoconjugates, offering significant potential for biotechnological applications. Through genomic data mining, we have identified and characterized a suite of β-1,3-galactosyltransferase enzymes, pivotal for the synthesis of glycan structures. Out of ten cloned gene candidates, six enzymes were successfully expressed recombinantly in Escherichia coli. Four of these enzymes exhibited measurable catalytic activity in the transfer of galactose to various acceptor substrates. Notably, MgB3GalT1 demonstrated the highest efficiency, achieving a 91.2 % conversion rate. This enzyme was proficient in glycosylating diverse glycan structures, including Core 2 O-glycans and several di-, tri-, and tetra-antennary complex N-glycan standards. Mass spectrometric analysis confirmed the successful modification of N-glycans. These findings open new approaches for utilizing oyster-derived enzymes in glycan-based therapeutics and molecular glycoengineering, highlighting their utility in synthetic applications and biotechnological advancements.

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来源期刊
Carbohydrate Research
Carbohydrate Research 化学-生化与分子生物学
CiteScore
5.00
自引率
3.20%
发文量
183
审稿时长
3.6 weeks
期刊介绍: Carbohydrate Research publishes reports of original research in the following areas of carbohydrate science: action of enzymes, analytical chemistry, biochemistry (biosynthesis, degradation, structural and functional biochemistry, conformation, molecular recognition, enzyme mechanisms, carbohydrate-processing enzymes, including glycosidases and glycosyltransferases), chemical synthesis, isolation of natural products, physicochemical studies, reactions and their mechanisms, the study of structures and stereochemistry, and technological aspects. Papers on polysaccharides should have a "molecular" component; that is a paper on new or modified polysaccharides should include structural information and characterization in addition to the usual studies of rheological properties and the like. A paper on a new, naturally occurring polysaccharide should include structural information, defining monosaccharide components and linkage sequence. Papers devoted wholly or partly to X-ray crystallographic studies, or to computational aspects (molecular mechanics or molecular orbital calculations, simulations via molecular dynamics), will be considered if they meet certain criteria. For computational papers the requirements are that the methods used be specified in sufficient detail to permit replication of the results, and that the conclusions be shown to have relevance to experimental observations - the authors'' own data or data from the literature. Specific directions for the presentation of X-ray data are given below under Results and "discussion".
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