从蒙古未接种疫苗的狗身上分离出的犬瘟热病毒的基因组测序

IF 2.3 2区 农林科学 Q1 VETERINARY SCIENCES Veterinary journal Pub Date : 2024-08-28 DOI:10.1016/j.tvjl.2024.106231
Ariunbold Munkhtsetseg , Enkhbaatar Batmagnai , Myagmarsuren Odonchimeg , Gombodash Ganbat , Yondonjamts Enkhmandakh , Gantulga Ariunbold , Tsedenbal Dolgorsuren , Raadan Odbileg , Purevtseren Dulam , Bumduuren Tuvshintulga , Chihiro Sugimoto , Yoshihiro Sakoda , Junya Yamagishi , Dashzevge Erdenechimeg
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引用次数: 0

摘要

犬瘟热病毒(CDV)在犬和野生动物中引发一种严重的、往往致命的疾病,即犬瘟热(CD)。先前的研究指出,来自不同地理区域的 CDV 分离物之间存在显著的遗传多样性和重组,这可能是导致疫苗失效的原因之一。尽管如此,尚未对蒙古 CDV 进行基因鉴定。这项研究从三只未接种疫苗的狗身上分离出了 CDV:两只 10 个月大的混血犬和一只 18 个月大的萨摩耶犬。它们都出现了 CD 症状,随后死亡。使用 Vero/dog SLAM 细胞进行病毒分离,并通过纳米孔技术进行基因组测序。混种犬感染的是非重组 CDV 分离物,与亚洲流行的亚洲-1 系形成姊妹支系。萨摩耶犬感染的是非重组 CDV 分离物,属于亚洲-4 系,在一些亚洲国家偶有报道。这些测序数据为遗传多样性提供了基础信息,有助于CD控制措施的制定,并有利于未来欧亚大陆和亚洲的研究。
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Genome sequencing of canine distemper virus isolates from unvaccinated dogs in Mongolia

Canine distemper virus (CDV) triggers a severe, often fatal disease in dogs and wildlife known as canine distemper (CD). Prior research has noted significant genetic diversity and recombination among CDV isolates from different geographical regions, potentially contributing to vaccine failures. Despite this, no genetic characterization of Mongolian CDVs has been conducted. This study, isolated CDVs from three unvaccinated dogs: two 10-month-old mixed-breeds and an 18-month-old Samoyed. All exhibited CD symptoms and subsequently died. Virus isolation was conducted using Vero/dog SLAM cells, with genome sequencing performed via nanopore technology. The mixed-breed dogs were infected with non-recombinant CDV isolates, forming a sister clade to the Asia-1 lineage prevalent in Asia. The Samoyed was infected with a non-recombinant CDV isolate, classifying as Asia-4 lineage sporadically reported in some Asian countries. This sequencing data offers foundational information on genetic diversity, aiding CD control measure development and benefiting future Eurasia and Asian studies.

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来源期刊
Veterinary journal
Veterinary journal 农林科学-兽医学
CiteScore
4.10
自引率
4.50%
发文量
79
审稿时长
40 days
期刊介绍: The Veterinary Journal (established 1875) publishes worldwide contributions on all aspects of veterinary science and its related subjects. It provides regular book reviews and a short communications section. The journal regularly commissions topical reviews and commentaries on features of major importance. Research areas include infectious diseases, applied biochemistry, parasitology, endocrinology, microbiology, immunology, pathology, pharmacology, physiology, molecular biology, immunogenetics, surgery, ophthalmology, dermatology and oncology.
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