糖生物碱对甲虫抗氧化系统的调控

IF 3.9 3区 环境科学与生态学 Q2 BIOCHEMISTRY & MOLECULAR BIOLOGY Comparative Biochemistry and Physiology C-toxicology & Pharmacology Pub Date : 2024-08-30 DOI:10.1016/j.cbpc.2024.110018
{"title":"糖生物碱对甲虫抗氧化系统的调控","authors":"","doi":"10.1016/j.cbpc.2024.110018","DOIUrl":null,"url":null,"abstract":"<div><p>Various factors may affect the antioxidative system in insects, including xenobiotics. Glycoalkaloids (GAs) are plant secondary metabolites produced mainly by the <em>Solanacea</em>e family (nightshades), such as the food crop tomato <em>Solanum lycopersicum</em> L. These compounds exhibit a wide range of biological activities and have attracted increasing interest in the context of potential insecticide properties. Therefore, the aim of the presented study was to analyze the effects of GAs (solanine, chaconine, tomatine, and extracts of tomato leaves) on lipid peroxidation; the expression levels of genes encoding manganese superoxide dismutase (MnSOD), catalase (CAT), and heat shock protein 70 (HSP70); and the enzymatic activity of SOD and CAT in <em>Tenebrio molitor</em> larvae. This species is amodel organism for toxicological and ecophysiological studies and is also a pest of grain storage. The reported changes depend on the GA concentration, incubation time, and type of insect tissue. We observed that the tested GAs affected <em>MnSOD</em> expression levels, increased SOD activity in the fat body, and reduced enzyme activity in the gut. The results showed that <em>CAT</em> expression was upregulated in the fat body and that the enzymatic activity of CAT in the gut was greater in the treated group than in the control group. Moreover, GAs affected <em>HSP70</em> expression and malondialdehyde levels in both tested tissues. This research contributes to our knowledge about the effects of GAs on the antioxidative system of <em>T. molitor</em> beetles. As efficient antioxidative system functioning is necessary for survival, the tested components may be targets of potential bioinsecticides.</p></div>","PeriodicalId":10602,"journal":{"name":"Comparative Biochemistry and Physiology C-toxicology & Pharmacology","volume":null,"pages":null},"PeriodicalIF":3.9000,"publicationDate":"2024-08-30","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://www.sciencedirect.com/science/article/pii/S1532045624001868/pdfft?md5=df9109991aea3a1838d1ea06c917faac&pid=1-s2.0-S1532045624001868-main.pdf","citationCount":"0","resultStr":"{\"title\":\"Modulation of the antioxidant system by glycoalkaloids in the beetle Tenebrio molitor L.\",\"authors\":\"\",\"doi\":\"10.1016/j.cbpc.2024.110018\",\"DOIUrl\":null,\"url\":null,\"abstract\":\"<div><p>Various factors may affect the antioxidative system in insects, including xenobiotics. Glycoalkaloids (GAs) are plant secondary metabolites produced mainly by the <em>Solanacea</em>e family (nightshades), such as the food crop tomato <em>Solanum lycopersicum</em> L. These compounds exhibit a wide range of biological activities and have attracted increasing interest in the context of potential insecticide properties. Therefore, the aim of the presented study was to analyze the effects of GAs (solanine, chaconine, tomatine, and extracts of tomato leaves) on lipid peroxidation; the expression levels of genes encoding manganese superoxide dismutase (MnSOD), catalase (CAT), and heat shock protein 70 (HSP70); and the enzymatic activity of SOD and CAT in <em>Tenebrio molitor</em> larvae. This species is amodel organism for toxicological and ecophysiological studies and is also a pest of grain storage. The reported changes depend on the GA concentration, incubation time, and type of insect tissue. We observed that the tested GAs affected <em>MnSOD</em> expression levels, increased SOD activity in the fat body, and reduced enzyme activity in the gut. The results showed that <em>CAT</em> expression was upregulated in the fat body and that the enzymatic activity of CAT in the gut was greater in the treated group than in the control group. Moreover, GAs affected <em>HSP70</em> expression and malondialdehyde levels in both tested tissues. This research contributes to our knowledge about the effects of GAs on the antioxidative system of <em>T. molitor</em> beetles. As efficient antioxidative system functioning is necessary for survival, the tested components may be targets of potential bioinsecticides.</p></div>\",\"PeriodicalId\":10602,\"journal\":{\"name\":\"Comparative Biochemistry and Physiology C-toxicology & Pharmacology\",\"volume\":null,\"pages\":null},\"PeriodicalIF\":3.9000,\"publicationDate\":\"2024-08-30\",\"publicationTypes\":\"Journal Article\",\"fieldsOfStudy\":null,\"isOpenAccess\":false,\"openAccessPdf\":\"https://www.sciencedirect.com/science/article/pii/S1532045624001868/pdfft?md5=df9109991aea3a1838d1ea06c917faac&pid=1-s2.0-S1532045624001868-main.pdf\",\"citationCount\":\"0\",\"resultStr\":null,\"platform\":\"Semanticscholar\",\"paperid\":null,\"PeriodicalName\":\"Comparative Biochemistry and Physiology C-toxicology & Pharmacology\",\"FirstCategoryId\":\"93\",\"ListUrlMain\":\"https://www.sciencedirect.com/science/article/pii/S1532045624001868\",\"RegionNum\":3,\"RegionCategory\":\"环境科学与生态学\",\"ArticlePicture\":[],\"TitleCN\":null,\"AbstractTextCN\":null,\"PMCID\":null,\"EPubDate\":\"\",\"PubModel\":\"\",\"JCR\":\"Q2\",\"JCRName\":\"BIOCHEMISTRY & MOLECULAR BIOLOGY\",\"Score\":null,\"Total\":0}","platform":"Semanticscholar","paperid":null,"PeriodicalName":"Comparative Biochemistry and Physiology C-toxicology & Pharmacology","FirstCategoryId":"93","ListUrlMain":"https://www.sciencedirect.com/science/article/pii/S1532045624001868","RegionNum":3,"RegionCategory":"环境科学与生态学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":null,"EPubDate":"","PubModel":"","JCR":"Q2","JCRName":"BIOCHEMISTRY & MOLECULAR BIOLOGY","Score":null,"Total":0}
引用次数: 0

摘要

影响昆虫抗氧化系统的因素有很多,其中包括异种生物。糖类生物碱(GAs)是植物的次级代谢产物,主要由茄科植物(夜茄属)产生,如粮食作物番茄(Solanum lycopersicum L.),这些化合物具有广泛的生物活性,在潜在的杀虫特性方面引起了越来越多的兴趣。因此,本研究旨在分析茄碱(茄碱、夏康碱、番茄碱和番茄叶提取物)对番茄幼虫体内脂质过氧化反应、编码锰超氧化物歧化酶(MnSOD)、过氧化氢酶(CAT)和热休克蛋白 70(HSP70)的基因表达水平以及 SOD 和 CAT 酶活性的影响。该物种是毒理学和生态生理学研究的模型生物,也是谷物储藏中的害虫。所报告的变化取决于 GA 浓度、孵化时间和昆虫组织类型。我们观察到,测试的雌激素影响了 MnSOD 的表达水平,增加了脂肪体中的 SOD 活性,降低了肠道中的酶活性。结果表明,与对照组相比,处理组脂肪体中 CAT 的表达上调,肠道中 CAT 的酶活性增强。此外,γ-氨基丁酸还影响了两种受测组织中 HSP70 的表达和丙二醛的水平。这项研究有助于我们了解 GA 对甲虫抗氧化系统的影响。由于高效的抗氧化系统是甲虫生存的必要条件,因此所测试的成分可能是潜在生物杀虫剂的目标。
本文章由计算机程序翻译,如有差异,请以英文原文为准。

摘要图片

查看原文
分享 分享
微信好友 朋友圈 QQ好友 复制链接
本刊更多论文
Modulation of the antioxidant system by glycoalkaloids in the beetle Tenebrio molitor L.

Various factors may affect the antioxidative system in insects, including xenobiotics. Glycoalkaloids (GAs) are plant secondary metabolites produced mainly by the Solanaceae family (nightshades), such as the food crop tomato Solanum lycopersicum L. These compounds exhibit a wide range of biological activities and have attracted increasing interest in the context of potential insecticide properties. Therefore, the aim of the presented study was to analyze the effects of GAs (solanine, chaconine, tomatine, and extracts of tomato leaves) on lipid peroxidation; the expression levels of genes encoding manganese superoxide dismutase (MnSOD), catalase (CAT), and heat shock protein 70 (HSP70); and the enzymatic activity of SOD and CAT in Tenebrio molitor larvae. This species is amodel organism for toxicological and ecophysiological studies and is also a pest of grain storage. The reported changes depend on the GA concentration, incubation time, and type of insect tissue. We observed that the tested GAs affected MnSOD expression levels, increased SOD activity in the fat body, and reduced enzyme activity in the gut. The results showed that CAT expression was upregulated in the fat body and that the enzymatic activity of CAT in the gut was greater in the treated group than in the control group. Moreover, GAs affected HSP70 expression and malondialdehyde levels in both tested tissues. This research contributes to our knowledge about the effects of GAs on the antioxidative system of T. molitor beetles. As efficient antioxidative system functioning is necessary for survival, the tested components may be targets of potential bioinsecticides.

求助全文
通过发布文献求助,成功后即可免费获取论文全文。 去求助
来源期刊
CiteScore
7.50
自引率
5.10%
发文量
206
审稿时长
30 days
期刊介绍: Part C: Toxicology and Pharmacology. This journal is concerned with chemical and drug action at different levels of organization, biotransformation of xenobiotics, mechanisms of toxicity, including reactive oxygen species and carcinogenesis, endocrine disruptors, natural products chemistry, and signal transduction with a molecular approach to these fields.
期刊最新文献
Assessing antioxidant responses in C6 and U-87 MG cell lines exposed to high copper levels. Deficiency of PvDRAM2 increased the nitrite sensitivity of Pacific white shrimp (Penaeus vannamei) by inhibiting autophagy. Effect of lead on photosynthetic pigments, antioxidant responses, metabolomics, thalli morphology and cell ultrastructure of Iridaea cordata (Rhodophyta) from Antarctica Tire rubber-derived contaminant 6PPD had the potential to induce metabolism disorder in early developmental stage of zebrafish Effects of water immersion on immune, intestinal flora and metabolome of Chinese mitten crab (Eriocheir sinensis) after air exposure
×
引用
GB/T 7714-2015
复制
MLA
复制
APA
复制
导出至
BibTeX EndNote RefMan NoteFirst NoteExpress
×
×
提示
您的信息不完整,为了账户安全,请先补充。
现在去补充
×
提示
您因"违规操作"
具体请查看互助需知
我知道了
×
提示
现在去查看 取消
×
提示
确定
0
微信
客服QQ
Book学术公众号 扫码关注我们
反馈
×
意见反馈
请填写您的意见或建议
请填写您的手机或邮箱
已复制链接
已复制链接
快去分享给好友吧!
我知道了
×
扫码分享
扫码分享
Book学术官方微信
Book学术文献互助
Book学术文献互助群
群 号:481959085
Book学术
文献互助 智能选刊 最新文献 互助须知 联系我们:info@booksci.cn
Book学术提供免费学术资源搜索服务,方便国内外学者检索中英文文献。致力于提供最便捷和优质的服务体验。
Copyright © 2023 Book学术 All rights reserved.
ghs 京公网安备 11010802042870号 京ICP备2023020795号-1