同时使用文拉法辛和钙通道阻滞剂对吗啡耐受性的影响:线粒体损伤和大脑氧化应激的作用。

IF 3.3 3区 心理学 Q1 BEHAVIORAL SCIENCES Pharmacology Biochemistry and Behavior Pub Date : 2024-08-30 DOI:10.1016/j.pbb.2024.173864
Asma Soleimanii , Faezeh Fallah , Behnam Ghorbanzadeh , Ali Akbar Oroojan , Neda Amirgholami , Soheila Alboghobeish
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引用次数: 0

摘要

背景:对吗啡产生耐受性的原因之一是氧化应激增加和海马细胞线粒体功能失调。文拉法辛和钙通道阻滞剂可以保护线粒体功能。本研究评估了线粒体损伤和氧化应激在同时使用文拉法辛和钙通道阻滞剂对吗啡急性镇痛效应的作用以及对吗啡效应耐受性的诱导:在这项实验研究中,为了诱导对吗啡的耐受性,NMRI小鼠连续三天接受50毫克/千克吗啡的治疗,第四天接受5毫克/千克吗啡的治疗。在注射吗啡前30分钟单独或与钙通道阻滞剂尼莫地平(10毫克/千克)和地尔硫卓(40毫克/千克)联合使用文拉法辛(20毫克/千克),并进行热板试验。然后用差速离心法分离海马线粒体,测定线粒体脱氢酶活性、线粒体膜电位、线粒体ROS产生率以及海马线粒体中谷胱甘肽和丙二醛的含量:结果:服用文拉法辛-尼莫地平和文拉法辛-地尔硫卓能增加吗啡的急性镇痛效果(P 讨论和结论:我们的数据表明,文拉法辛与钙通道阻滞剂同时使用可增加吗啡的急性镇痛效果,并减少吗啡耐受性的诱导和表现。此外,同时服用文拉法辛和钙通道阻滞剂对吗啡在耐受试验中诱导的线粒体氧化应激和损伤具有预防和保护作用。
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Simultaneous use of venlafaxine and calcium channel blockers on tolerance to morphine: The role of mitochondrial damage and oxidative stress in the brain

Background

One of the reasons for tolerance to morphine is increased oxidative stress and dysfunction of cell mitochondria in the hippocampus. Venlafaxine and calcium channel blockers can protect mitochondrial function. The investigation of the role of mitochondrial damage and oxidative stress in the simultaneous use of venlafaxine and calcium channel blockers on the acute analgesic effects of morphine and the induction of tolerance to its effects in mice was assessed.

Method

In this experimental study, to induce tolerance to morphine, NMRI mice were treated with 50 mg/kg morphine for three consecutive days and 5 mg/kg morphine on the fourth day. Venlafaxine (20 mg/kg) alone or in combination with calcium channel blockers, nimodipine (10 mg/kg), and diltiazem (40 mg/kg) was administered 30 min before morphine, and the hot plate test was used. Then, hippocampal mitochondria were isolated by differential centrifugation method, and the levels of mitochondrial dehydrogenase activity, mitochondrial membrane potential, mitochondrial ROS production rate, as well as the content of glutathione and malondialdehyde in hippocampal mitochondria, were measured.

Results

The administration of venlafaxine-nimodipine and venlafaxine-diltiazem increased morphine's acute analgesic effects (P < 0.05) and reduced the induction and expression of tolerance to the analgesic effects of morphine (P < 0.05). Morphine significantly decreased MTT and GSH and increased MDA, mitochondrial membrane damage, and ROS compared to the control group (P < 0.01). Injection of venlafaxine-nimodipine and also venlafaxine-diltiazem 30 min before morphine can improve these alterations (P < 0.05).

Discussion and conclusion

Our data showed that the simultaneous use of venlafaxine with calcium channel blockers could increase the acute analgesic effects of morphine and reduce the induction and expression of tolerance to it. Also, the preventive and protective roles of simultaneous administration of venlafaxine and calcium channel blockers on morphine-induced mitochondrial oxidative stress and damage during the tolerance test were achieved.

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来源期刊
CiteScore
6.40
自引率
2.80%
发文量
122
审稿时长
38 days
期刊介绍: Pharmacology Biochemistry & Behavior publishes original reports in the areas of pharmacology and biochemistry in which the primary emphasis and theoretical context are behavioral. Contributions may involve clinical, preclinical, or basic research. Purely biochemical or toxicology studies will not be published. Papers describing the behavioral effects of novel drugs in models of psychiatric, neurological and cognitive disorders, and central pain must include a positive control unless the paper is on a disease where such a drug is not available yet. Papers focusing on physiological processes (e.g., peripheral pain mechanisms, body temperature regulation, seizure activity) are not accepted as we would like to retain the focus of Pharmacology Biochemistry & Behavior on behavior and its interaction with the biochemistry and neurochemistry of the central nervous system. Papers describing the effects of plant materials are generally not considered, unless the active ingredients are studied, the extraction method is well described, the doses tested are known, and clear and definite experimental evidence on the mechanism of action of the active ingredients is provided.
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