试点研究:通过转录谱了解犬传染性性病肿瘤。

IF 1.4 3区 农林科学 Q4 IMMUNOLOGY Veterinary immunology and immunopathology Pub Date : 2024-08-30 DOI:10.1016/j.vetimm.2024.110818
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引用次数: 0

摘要

犬传染性性病肿瘤(CTVT)通过肿瘤细胞的植入传播。CTVT是第一个被描述为具有传染性特征的肿瘤,目前仍是少数具有这种能力的肿瘤之一。本研究旨在绘制 CTVT 的转录组图谱,以阐明这种肿瘤植入和逃避宿主免疫监视的潜在机制。本研究选择了 11 只年龄≥ 2 岁、确诊为 CTVT 的狗。对肿瘤进行活检,提取 RNA 并将其转化为互补 DNA,然后进行 RT-qPCR 分析。CTVT 的转录组图谱显示了一系列差异表达基因。不过,本文仅从肿瘤学角度讨论了最相关的基因。IL-8、CXCL13、NCAM1、RNASEL、COROA1 和 CBLB 显示了与免疫系统逃避和通过植入传播的潜在关联。因此,对这些基因的研究可能有助于开发防止传染和免疫逃避的靶向疗法。
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Pilot study: Understanding canine transmissible venereal tumor through its transcriptional profile

Canine transmissible venereal tumor (CTVT) is transmitted through the implantation of tumor cells. CTVT was the first tumor described with contagious characteristics and remains one of the few tumors with this capability. This study aimed to map the transcriptomic profile of CTVT to elucidate the potential mechanisms through which this tumor implants and evades host immune surveillance. For this study, 11 dogs aged ≥ 2 years diagnosed with CTVT were selected. Tumor biopsies were performed, RNA was extracted and converted into complementary DNA, followed by RT-qPCR analysis. The transcriptomic profile of CTVT revealed a wide array of differentially expressed genes. However, only the most relevant genes from an oncological perspective were discussed. IL-8, CXCL13, NCAM1, RNASEL, COROA1, and CBLB demonstrated potential associations with immune system evasion and transmission via implantation. Therefore, studying these genes may contribute to the development of targeted therapies that prevent contagion and immune evasion.

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来源期刊
CiteScore
3.40
自引率
5.60%
发文量
79
审稿时长
70 days
期刊介绍: The journal reports basic, comparative and clinical immunology as they pertain to the animal species designated here: livestock, poultry, and fish species that are major food animals and companion animals such as cats, dogs, horses and camels, and wildlife species that act as reservoirs for food, companion or human infectious diseases, or as models for human disease. Rodent models of infectious diseases that are of importance in the animal species indicated above,when the disease requires a level of containment that is not readily available for larger animal experimentation (ABSL3), will be considered. Papers on rabbits, lizards, guinea pigs, badgers, armadillos, elephants, antelope, and buffalo will be reviewed if the research advances our fundamental understanding of immunology, or if they act as a reservoir of infectious disease for the primary animal species designated above, or for humans. Manuscripts employing other species will be reviewed if justified as fitting into the categories above. The following topics are appropriate: biology of cells and mechanisms of the immune system, immunochemistry, immunodeficiencies, immunodiagnosis, immunogenetics, immunopathology, immunology of infectious disease and tumors, immunoprophylaxis including vaccine development and delivery, immunological aspects of pregnancy including passive immunity, autoimmuity, neuroimmunology, and transplanatation immunology. Manuscripts that describe new genes and development of tools such as monoclonal antibodies are also of interest when part of a larger biological study. Studies employing extracts or constituents (plant extracts, feed additives or microbiome) must be sufficiently defined to be reproduced in other laboratories and also provide evidence for possible mechanisms and not simply show an effect on the immune system.
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