评估使用线环浮选法检测胃肠道寄生虫的方法。

IF 1.8 3区 农林科学 Q2 VETERINARY SCIENCES Veterinary Medicine and Science Pub Date : 2024-09-01 DOI:10.1002/vms3.70007
Aruto Takano, Daikichi Morinaga, Isao Teramoto, Toshimitsu Hatabu, Yasutoshi Kido, Akira Kaneko, Takeshi Hatta, Naotoshi Tsuji, Shigehiko Uni, Kazumi Sasai, Hiromitsu Katoh, Makoto Matsubayashi
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引用次数: 0

摘要

胃肠道寄生虫感染在世界各地的各种动物中都有发现。在诊断这类感染时,通常采用浮选法检测粪便中的寄生虫微生物,如卵囊或虫卵。作为替代方法之一,有报道称使用铁丝圈回收微生物的方法取代了在最后离心步骤后添加浮选溶液和使用盖玻片收集寄生虫的方法。不过,尚未对浮选法的微生物回收率进行分析。在本研究中,我们使用不同数量的天牛卵囊和五倍子母鸡卵以及在养殖场收集的鸡粪便样本,评估了使用直径为 8 毫米的金属丝圈(金属丝圈法)进行浮选的方法的实用性。结果发现,管内卵囊和虫卵的收集比例为 2.00 到 3.08。因此,我们的结果表明,循环法是一种简单、省时的方法,可用于估算样本的 OPG/EPG(每克卵囊数/虫卵数)。
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Evaluation of the detection method by a flotation method using a wire loop for gastrointestinal parasites.

Infections by gastrointestinal parasites are found in a variety of animals worldwide. For the diagnosis of such infections, the flotation method is commonly used to detect parasitic microorganisms, such as oocysts or eggs, in feces. Instead of adding a flotation solution after the final centrifugation step and using a cover slip to collect the parasites, the method using a wire loop for the recovery of the organisms has been reported as one of alternative methods. However, the recovery rates of microorganisms from the flotation method have not been analysed. In the present study, the utility of a flotation method with the use of a wire loop of 8 mm in diameter (the loop method) was evaluated using different numbers of E. tenella oocysts and Heterakis gallinarum eggs, and chicken fecal samples collected at the farms. Consequently, we found that the oocysts and eggs in tubes could be collected at a ratio of 2.00 to 3.08. Thus, our results indicate that the loop method is a simple and time saving method, implicating the application for the estimated OPG/ EPG (Oocysts/Eggs per gram) of the samples.

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来源期刊
Veterinary Medicine and Science
Veterinary Medicine and Science Veterinary-General Veterinary
CiteScore
3.00
自引率
0.00%
发文量
296
期刊介绍: Veterinary Medicine and Science is the peer-reviewed journal for rapid dissemination of research in all areas of veterinary medicine and science. The journal aims to serve the research community by providing a vehicle for authors wishing to publish interesting and high quality work in both fundamental and clinical veterinary medicine and science. Veterinary Medicine and Science publishes original research articles, systematic reviews, meta-analyses, and research methods papers, along with invited editorials and commentaries. Original research papers must report well-conducted research with conclusions supported by the data presented in the paper. We aim to be a truly global forum for high-quality research in veterinary medicine and science, and believe that the best research should be published and made widely accessible as quickly as possible. Veterinary Medicine and Science publishes papers submitted directly to the journal and those referred from a select group of prestigious journals published by Wiley-Blackwell. Veterinary Medicine and Science is a Wiley Open Access journal, one of a new series of peer-reviewed titles publishing quality research with speed and efficiency. For further information visit the Wiley Open Access website.
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