雄性大鼠交配后头两天尿液蛋白质组的比较

Advances in biomarker sciences and technology Pub Date : 2024-01-01 DOI:10.1016/j.abst.2024.08.003

Haitong Wang, Chenyang Zhao, Youhe Gao

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引用次数: 0

摘要

目的探讨雄性大鼠在交配次日和交配当日的差异是否能通过尿液蛋白质组反映出来。方法收集雄性 Sprague-Dawley 大鼠在交配当日和交配次日的尿液样本。采用高效液相色谱串联质谱（LC-MS/MS）无标记定量蛋白质组学技术对尿液样本进行分析。结果通过比较大鼠交配次日与交配当日的尿液蛋白质组，发现了54种差异蛋白质，其中近三分之二的差异蛋白质与精子发生有关。

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Comparison of urinary proteome in the first two days after mating in male rats

Objective

To explore whether differences between male rats on the next day of mating and on the day of mating can be reflected by the urine proteome.

Methods

Urine samples were collected from male Sprague-Dawley rats on the day of mating and the next day of mating. Urine samples were analysed by the label-free quantitative proteomics technique of high-performance liquid chromatography tandem mass spectrometry (LC-MS/MS). Differential proteins of the urine proteome were analysed for protein function and biological pathways.

Results

54 differential proteins were identified by comparing the urine proteome of rats on the next day of mating with that on the day of mating, and nearly two-thirds of the differential proteins were related to spermatogenesis.