Shichen Han, Yulong Wang, Yingxiu Li, Rui Zhu, Yunsong Gu, Jin Li, Haifeng Guo, Wei Ye, Hafiz Ghualm Nabi, Tao Yang, Yanming Wang, Pengli Liu, Junzhi Duan, Xingming Sun, Zhanying Zhang, Hongliang Zhang, Zichao Li, Jinjie Li
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We found that a G-to-T polymorphism in the RoLe1 promoter causes increased binding of the transcription factor OsNAC41 and thereby enhanced expression of RoLe1. We further showed that RoLe1 interacts with OsAGAP, an ARF-GTPase activating protein involved in auxin-dependent root development, and interferes with its function to modulate root development. Interestingly, RoLe1 could enhance crop yield by increasing the seed-setting rate under moderate drought conditions. Genomic evolutionary analysis revealed that a newly arisen favorable allelic variant, proRoLe1<sup>-526T</sup>, originated from the midwest Asia and was retained in upland rice during domestication. Collectively, our study identifies an OsNAC41-RoLe1-OsAGAP module that promotes upland rice root development and drought resistance, providing promising genetic targets for molecular breeding of drought-resistant rice varieties.</p>","PeriodicalId":19012,"journal":{"name":"Molecular Plant","volume":null,"pages":null},"PeriodicalIF":17.1000,"publicationDate":"2024-10-07","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":"0","resultStr":"{\"title\":\"The OsNAC41-RoLe1-OsAGAP module promotes root development and drought resistance in upland rice.\",\"authors\":\"Shichen Han, Yulong Wang, Yingxiu Li, Rui Zhu, Yunsong Gu, Jin Li, Haifeng Guo, Wei Ye, Hafiz Ghualm Nabi, Tao Yang, Yanming Wang, Pengli Liu, Junzhi Duan, Xingming Sun, Zhanying Zhang, Hongliang Zhang, Zichao Li, Jinjie Li\",\"doi\":\"10.1016/j.molp.2024.09.002\",\"DOIUrl\":null,\"url\":null,\"abstract\":\"<p><p>Drought is a major environmental stress limiting crop yields worldwide. 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引用次数: 0
摘要
干旱是限制全球作物产量的主要环境胁迫。陆稻(Oryza sativa)已进化出复杂的遗传机制来适应干旱胁迫。然而,在陆地水稻中发现的介导抗旱性的遗传变异很少,人们对这一性状在驯化过程中的进化也知之甚少。在此,我们利用水稻的全基因组关联研究,确定了控制根长和抗旱性的根长 1(RoLe1)。我们证明,RoLe1 启动子中的 G 到 T 多态性增加了转录因子 OsNAC41 的结合,从而激活了其转录。我们还发现,RoLe1 与参与辅助素依赖性根系发育的 ARF-GTPase 激活蛋白 OsAGAP 相互作用并干扰其功能,从而调节根系发育。此外,在中度干旱条件下,RoLe1通过提高种子结实率提高了作物产量。基因组进化分析表明,一个新出现的有利等位基因变体 proRoLe1-526T 起源于 I 区(亚洲中西部),并在驯化过程中保留在高原水稻中。我们的研究结果提出了一个 OsNAC41-RoLe1-OsAGAP 模块,为水稻抗旱品种的分子育种提供了有前景的遗传目标。
The OsNAC41-RoLe1-OsAGAP module promotes root development and drought resistance in upland rice.
Drought is a major environmental stress limiting crop yields worldwide. Upland rice (Oryza sativa) has evolved complex genetic mechanisms for adaptative growth under drought stress. However, few genetic variants that mediate drought resistance in upland rice have been identified, and little is known about the evolution of this trait during rice domestication. In this study, using a genome-wide association study we identified ROOT LENGTH 1 (RoLe1) that controls rice root length and drought resistance. We found that a G-to-T polymorphism in the RoLe1 promoter causes increased binding of the transcription factor OsNAC41 and thereby enhanced expression of RoLe1. We further showed that RoLe1 interacts with OsAGAP, an ARF-GTPase activating protein involved in auxin-dependent root development, and interferes with its function to modulate root development. Interestingly, RoLe1 could enhance crop yield by increasing the seed-setting rate under moderate drought conditions. Genomic evolutionary analysis revealed that a newly arisen favorable allelic variant, proRoLe1-526T, originated from the midwest Asia and was retained in upland rice during domestication. Collectively, our study identifies an OsNAC41-RoLe1-OsAGAP module that promotes upland rice root development and drought resistance, providing promising genetic targets for molecular breeding of drought-resistant rice varieties.
期刊介绍:
Molecular Plant is dedicated to serving the plant science community by publishing novel and exciting findings with high significance in plant biology. The journal focuses broadly on cellular biology, physiology, biochemistry, molecular biology, genetics, development, plant-microbe interaction, genomics, bioinformatics, and molecular evolution.
Molecular Plant publishes original research articles, reviews, Correspondence, and Spotlights on the most important developments in plant biology.