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引用次数: 0
摘要
从中国中部河南省天池山采集的植物叶片中分离出两株酵母菌株：NYNU 236122和NYNU 236180。分子系统进化分析表明，这两株菌株的近缘种是三个已描述过的 Kondoa 物种：Kondoa chamaenerii、Kondoa miscanthi 和 Kondoa subrosea。从遗传学角度看，分离的菌株与这三个亲缘种的模式菌株存在以下差异：D1/D2结构域有2-11（0.2-1.8%）个碱基替换，内部转录间隔区有16-40（2.6-5.6%）个碱基错配，部分RPB2基因有超过10.1%的碱基替换。此外，这两株菌株在生理上与其最亲缘的物种 K. chamaenerii 不同，它们能同化 dl-乳酸盐、亚硝酸盐和 l-赖氨酸，但不能同化硝酸盐。此外，它们与 K. miscanthi 和 K. subrosea 在同化菊粉、d-葡萄糖酸盐和 l-赖氨酸的能力上也有所不同。Kondoa tianchiensis f.a.，sp. nov.的种名，主模式为 CICC 33616T（Mycobank MB 853544）。
Kondoa tianchiensis f.a., sp. nov., an anamorphic yeast species isolated from plant leaves.
Two yeast strains, NYNU 236122 and NYNU 236180, were isolated from plant leaves collected in Tianchi Mountain, Henan Province, central China. Molecular phylogenetic analyses revealed the closest relatives of the strains are three described Kondoa species, Kondoa chamaenerii, Kondoa miscanthi, and Kondoa subrosea. Genetically, the isolated strains differed from the type strains of their three related species by 2-11(0.2-1.8%) base substitutions in the D1/D2 domain, 16-40 (2.6-5.6%) base mismatches in the internal transcribed spacer region, and more than 10.1% base substitutions in the partial RPB2 gene. Furthermore, the two strains differ physiologically from their closest related species, K. chamaenerii, in their ability to assimilate dl-lactate, nitrite, and l-lysine and their inability to assimilate nitrate. Additionally, they differ from K. miscanthi and K. subrosea in their ability to assimilate inulin, d-gluconate, and l-lysine. The species name of Kondoa tianchiensis f.a., sp. nov. is proposed with holotype CICC 33616T (Mycobank MB 853544).
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