GmBSK1-GmGSK1-GmBES1.5 调控模块控制大豆的耐热性。

Ze-Hao Hou, Yuan Gao, Jia-Cheng Zheng, Meng-Jie Zhao, Ying Liu, Xiao-Yu Cui, Zhi-Yong Li, Ji-Tong Wei, Tai-Fei Yu, Lei Zheng, Yuan-Chen Jiao, Shu-Hui Yang, Jia-Min Hao, Jun Chen, Yong-Bin Zhou, Ming Chen, Lijuan Qiu, You-Zhi Ma, Zhao-Shi Xu
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引用次数: 0

摘要

引言热胁迫对大豆(Glycine max)的生长和产量构成严重威胁。芸苔素类固醇(BRs)积极参与植物对非生物胁迫的响应,然而,BR 信号通路基因在大豆热胁迫响应中的作用仍鲜为人知:本研究探讨了 GmBSK1 和 GmBES1.5 对热胁迫的调控机制,以及热胁迫条件下大豆的生理特性和产量表现:利用转基因技术和CRISPR/Cas9技术产生GmBSK1-OE、GmBES1.5-OE和gmbsk1转基因大豆植株,并进行转录组分析、LUC活性检测和EMSA检测,以阐明GmBSK1-GmBES1.5介导的大豆耐热胁迫的潜在分子机制:结果:CRISPR/Cas9产生的gmbsk1基因敲除突变体由于清除活性氧(ROS)的能力降低,对热胁迫的敏感性增加。在热胁迫条件下,GmBSK1-OE植株中GmBES1.5的表达上调,它直接与非生物胁迫相关基因启动子中的E-box基序结合,从而增强了大豆植株的热胁迫耐受性。此外,我们还发现 GmGSK1 与 GmBES1.5 之间存在相互作用,而 GmGSK1 会抑制 GmBES1.5 的转录活性。有趣的是,GmBSK1 和 GmGSK1 之间的相互作用促进了 GmGSK1 在质膜上的定位,并释放了 GmBES1.5 的转录活性:我们的研究结果表明,GmBSK1 和 GmBES1.5 在赋予大豆热胁迫耐受性中起着至关重要的作用,这为培育耐热大豆作物提供了一种由 GmBSK1 -GmGSK1-GmBES1.5 组成的调控模块的潜在策略。
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GmBSK1-GmGSK1-GmBES1.5 regulatory module controls heat tolerance in soybean.

Introduction: Heat stress poses a severe threat to the growth and production of soybean (Glycine max). Brassinosteroids (BRs) actively participate in plant responses to abiotic stresses, however, the role of BR signaling pathway genes in response to heat stress in soybean remains poorly understood.

Objectives: In this study, we investigate the regulatory mechanisms of GmBSK1 and GmBES1.5 in response to heat stress and the physiological characteristics and yield performance under heat stress conditions.

Methods: Transgenic technology and CRISPR/Cas9 technology were used to generated GmBSK1-OE, GmBES1.5-OE and gmbsk1 transgenic soybean plants, and transcriptome analysis, LUC activity assay and EMSA assay were carried out to elucidate the potential molecular mechanism underlying GmBSK1-GmBES1.5-mediated heat stress tolerance in soybean.

Results: CRISPR/Cas9-generated gmbsk1 knockout mutants exhibited increased sensitivity to heat stress due to a reduction in their ability to scavenge reactive oxygen species (ROS). The expression of GmBES1.5 was up-regulated in GmBSK1-OE plants under heat stress conditions, and it directly binds to the E-box motif present in the promoters of abiotic stress-related genes, thereby enhancing heat stress tolerance in soybean plants. Furthermore, we identified an interaction between GmGSK1 and GmBES1.5, while GmGSK1 inhibits the transcriptional activity of GmBES1.5. Interestingly, the interaction between GmBSK1 and GmGSK1 promotes the localization of GmGSK1 to the plasma membrane and releases the transcriptional activity of GmBES1.5.

Conclusion: Our findings suggest that both GmBSK1 and GmBES1.5 play crucial roles in conferring heat stress tolerance, highlighting a potential strategy for breeding heat-tolerant soybean crops involving the regulatory module consisting of GmBSK1-GmGSK1-GmBES1.5.

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