{"title":"大肠杆菌冷休克表达系统中的蛋白质表达自诱导。","authors":"Yukino Tsujimoto, Naoto Isono","doi":"10.7171/3fc1f5fe.76009c9a","DOIUrl":null,"url":null,"abstract":"<p><p>The cold-shock expression system in <i>Escherichia coli</i> was developed on a manual induction approach using optical density at 600 nm (OD<sub>600</sub>) measurements and isopropyl β-D-1-thiogalactopyranoside (IPTG) addition. In this study, we show that cold-shock expression performs equally well using an autoinduction approach wherein OD<sub>600</sub> measurements and IPTG addition may be eliminated. We further demonstrate that cold-shock expression with autoinduction can better facilitate high-throughput experiments.</p>","PeriodicalId":94326,"journal":{"name":"Journal of biomolecular techniques : JBT","volume":"35 2","pages":""},"PeriodicalIF":0.0000,"publicationDate":"2024-04-29","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://www.ncbi.nlm.nih.gov/pmc/articles/PMC11373702/pdf/","citationCount":"0","resultStr":"{\"title\":\"Protein Expression Autoinduction in a Cold-Shock Expression System in Escherichia coli.\",\"authors\":\"Yukino Tsujimoto, Naoto Isono\",\"doi\":\"10.7171/3fc1f5fe.76009c9a\",\"DOIUrl\":null,\"url\":null,\"abstract\":\"<p><p>The cold-shock expression system in <i>Escherichia coli</i> was developed on a manual induction approach using optical density at 600 nm (OD<sub>600</sub>) measurements and isopropyl β-D-1-thiogalactopyranoside (IPTG) addition. In this study, we show that cold-shock expression performs equally well using an autoinduction approach wherein OD<sub>600</sub> measurements and IPTG addition may be eliminated. We further demonstrate that cold-shock expression with autoinduction can better facilitate high-throughput experiments.</p>\",\"PeriodicalId\":94326,\"journal\":{\"name\":\"Journal of biomolecular techniques : JBT\",\"volume\":\"35 2\",\"pages\":\"\"},\"PeriodicalIF\":0.0000,\"publicationDate\":\"2024-04-29\",\"publicationTypes\":\"Journal Article\",\"fieldsOfStudy\":null,\"isOpenAccess\":false,\"openAccessPdf\":\"https://www.ncbi.nlm.nih.gov/pmc/articles/PMC11373702/pdf/\",\"citationCount\":\"0\",\"resultStr\":null,\"platform\":\"Semanticscholar\",\"paperid\":null,\"PeriodicalName\":\"Journal of biomolecular techniques : JBT\",\"FirstCategoryId\":\"1085\",\"ListUrlMain\":\"https://doi.org/10.7171/3fc1f5fe.76009c9a\",\"RegionNum\":0,\"RegionCategory\":null,\"ArticlePicture\":[],\"TitleCN\":null,\"AbstractTextCN\":null,\"PMCID\":null,\"EPubDate\":\"2024/7/31 0:00:00\",\"PubModel\":\"eCollection\",\"JCR\":\"\",\"JCRName\":\"\",\"Score\":null,\"Total\":0}","platform":"Semanticscholar","paperid":null,"PeriodicalName":"Journal of biomolecular techniques : JBT","FirstCategoryId":"1085","ListUrlMain":"https://doi.org/10.7171/3fc1f5fe.76009c9a","RegionNum":0,"RegionCategory":null,"ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":null,"EPubDate":"2024/7/31 0:00:00","PubModel":"eCollection","JCR":"","JCRName":"","Score":null,"Total":0}
Protein Expression Autoinduction in a Cold-Shock Expression System in Escherichia coli.
The cold-shock expression system in Escherichia coli was developed on a manual induction approach using optical density at 600 nm (OD600) measurements and isopropyl β-D-1-thiogalactopyranoside (IPTG) addition. In this study, we show that cold-shock expression performs equally well using an autoinduction approach wherein OD600 measurements and IPTG addition may be eliminated. We further demonstrate that cold-shock expression with autoinduction can better facilitate high-throughput experiments.
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