生物电喷牛精子保持活力并使卵细胞受精

IF 11.9 1区 物理与天体物理 Q1 PHYSICS, APPLIED Applied physics reviews Pub Date : 2024-09-05 DOI:10.1063/5.0207391
Ali A. Fouladi-Nashta, Fataneh Ghafari, Walid E. Maalouf, Natalie J. Werling, Suwan N. Jayasinghe
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引用次数: 0

摘要

自发现生物电喷雾器以来,该技术经历了严格的开发过程,共接触了 600 多种细胞类型,包括原代、永生干细胞和整个受精胚胎。这些研究通过成熟的临床读数(流式细胞术、核型和基因芯片研究)和生物检测,将处理后的细胞与对照细胞(未暴露于生物电喷雾器的细胞)进行比较,证明生物电喷雾器能够直接安全地处理人类已知的最先进、最复杂的材料,即活细胞。由于我们之前的工作从分子水平证明了生物电喷雾器能够喷射人类精子和整个受精胚胎而不对其造成损害,因此我们希望研究喷射精子使卵细胞受精的能力是否会受到任何功能性影响。因此,在这些研究中,我们率先提出了这一问题,并首次证明,与对照组相比,生物电喷后的牛精子仍能保持运动和活力。这些研究为这一平台生物技术进入从生物模型开发、精子分析/分拣到精子保存等应用领域的研究铺平了道路。
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Bio-electrosprayed bovine sperm remain viable and fertilize oocytes
Since the discovery of bio-electrosprays, the technology has undergone a rigorous developmental program, which saw the technology exposing to well over 600 cell types ranging from primary, immortalized including stem cells to whole fertilized embryos. Those studies interrogated the post-treated cells in comparison to control cells (cells not exposed to bio-electrosprays) through both well-established clinical read outs (flow cytometry, karyotypic, and gene microarray studies) and biological assays, demonstrating the ability of bio-electrosprays to directly and safely handle the most advanced and complex materials known to humankind, namely, living cells. Since our previous work demonstrated bio-electrospray's ability to jet both human sperm and whole fertilized embryos without damaging them, from a molecular level upward, we wished to investigate if there are any functional effects brought on to the jetted sperm's ability to fertilize oocytes. Therefore, in these investigations, we spearheaded this question by demonstrating for the first time, post-bio-electrosprayed bovine sperm remains motile and viable as assessed, to finally retain their capacity to fertilize oocytes in comparison to controls. These studies pave the way for this platform biotechnology to enter investigations for applications ranging from the development of biological models, sperm analysis/sorting, to their preservation.
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来源期刊
Applied physics reviews
Applied physics reviews PHYSICS, APPLIED-
CiteScore
22.50
自引率
2.00%
发文量
113
审稿时长
2 months
期刊介绍: Applied Physics Reviews (APR) is a journal featuring articles on critical topics in experimental or theoretical research in applied physics and applications of physics to other scientific and engineering branches. The publication includes two main types of articles: Original Research: These articles report on high-quality, novel research studies that are of significant interest to the applied physics community. Reviews: Review articles in APR can either be authoritative and comprehensive assessments of established areas of applied physics or short, timely reviews of recent advances in established fields or emerging areas of applied physics.
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