Yang Sing Leong;Nur Aisyah Binti Kebehi;Mohd Hadri Hafiz Mokhtar;Mohd Saiful Dzulkefly Zan;Norhana Arsad;Ahmad Ashrif A. Bakar
{"title":"使用混合模式光纤对进行细胞分辨率成像的多通道共焦显微镜技术","authors":"Yang Sing Leong;Nur Aisyah Binti Kebehi;Mohd Hadri Hafiz Mokhtar;Mohd Saiful Dzulkefly Zan;Norhana Arsad;Ahmad Ashrif A. Bakar","doi":"10.1109/LPT.2024.3449697","DOIUrl":null,"url":null,"abstract":"This letter presents a study on the imaging capabilities of a mixed-mode fibre cantilever-based endoscopic scanner using cladding mode and multimode core detection methods. Characterization of the mixed-mode fibre cantilever was performed to determine the properties of the fibre cantilever and assess the capability of detection via the multimode core detection. Furthermore, we demonstrated the capabilities of these detection modes by imaging cellular samples. The findings reveal that cladding mode detection provides a bright image of the cellular structures with the \n<inline-formula> <tex-math>$16.8~\\mu $ </tex-math></inline-formula>\n m nucleus. Meanwhile, the images obtained from the multimode core detection reveal the irregularities on the surface, producing a pseudo-three-dimensional image of the sample. The application of multiple detection methods in the endoscopic scanner opens up new potential for more comprehensive optical imaging applications.","PeriodicalId":13065,"journal":{"name":"IEEE Photonics Technology Letters","volume":null,"pages":null},"PeriodicalIF":2.3000,"publicationDate":"2024-08-26","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":"0","resultStr":"{\"title\":\"Multichannel Confocal Microscopy Using Mixed-Mode Fiber Pair for Cellular-Resolution Imaging\",\"authors\":\"Yang Sing Leong;Nur Aisyah Binti Kebehi;Mohd Hadri Hafiz Mokhtar;Mohd Saiful Dzulkefly Zan;Norhana Arsad;Ahmad Ashrif A. Bakar\",\"doi\":\"10.1109/LPT.2024.3449697\",\"DOIUrl\":null,\"url\":null,\"abstract\":\"This letter presents a study on the imaging capabilities of a mixed-mode fibre cantilever-based endoscopic scanner using cladding mode and multimode core detection methods. Characterization of the mixed-mode fibre cantilever was performed to determine the properties of the fibre cantilever and assess the capability of detection via the multimode core detection. Furthermore, we demonstrated the capabilities of these detection modes by imaging cellular samples. The findings reveal that cladding mode detection provides a bright image of the cellular structures with the \\n<inline-formula> <tex-math>$16.8~\\\\mu $ </tex-math></inline-formula>\\n m nucleus. Meanwhile, the images obtained from the multimode core detection reveal the irregularities on the surface, producing a pseudo-three-dimensional image of the sample. The application of multiple detection methods in the endoscopic scanner opens up new potential for more comprehensive optical imaging applications.\",\"PeriodicalId\":13065,\"journal\":{\"name\":\"IEEE Photonics Technology Letters\",\"volume\":null,\"pages\":null},\"PeriodicalIF\":2.3000,\"publicationDate\":\"2024-08-26\",\"publicationTypes\":\"Journal Article\",\"fieldsOfStudy\":null,\"isOpenAccess\":false,\"openAccessPdf\":\"\",\"citationCount\":\"0\",\"resultStr\":null,\"platform\":\"Semanticscholar\",\"paperid\":null,\"PeriodicalName\":\"IEEE Photonics Technology Letters\",\"FirstCategoryId\":\"5\",\"ListUrlMain\":\"https://ieeexplore.ieee.org/document/10646380/\",\"RegionNum\":3,\"RegionCategory\":\"工程技术\",\"ArticlePicture\":[],\"TitleCN\":null,\"AbstractTextCN\":null,\"PMCID\":null,\"EPubDate\":\"\",\"PubModel\":\"\",\"JCR\":\"Q2\",\"JCRName\":\"ENGINEERING, ELECTRICAL & ELECTRONIC\",\"Score\":null,\"Total\":0}","platform":"Semanticscholar","paperid":null,"PeriodicalName":"IEEE Photonics Technology Letters","FirstCategoryId":"5","ListUrlMain":"https://ieeexplore.ieee.org/document/10646380/","RegionNum":3,"RegionCategory":"工程技术","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":null,"EPubDate":"","PubModel":"","JCR":"Q2","JCRName":"ENGINEERING, ELECTRICAL & ELECTRONIC","Score":null,"Total":0}
Multichannel Confocal Microscopy Using Mixed-Mode Fiber Pair for Cellular-Resolution Imaging
This letter presents a study on the imaging capabilities of a mixed-mode fibre cantilever-based endoscopic scanner using cladding mode and multimode core detection methods. Characterization of the mixed-mode fibre cantilever was performed to determine the properties of the fibre cantilever and assess the capability of detection via the multimode core detection. Furthermore, we demonstrated the capabilities of these detection modes by imaging cellular samples. The findings reveal that cladding mode detection provides a bright image of the cellular structures with the
$16.8~\mu $
m nucleus. Meanwhile, the images obtained from the multimode core detection reveal the irregularities on the surface, producing a pseudo-three-dimensional image of the sample. The application of multiple detection methods in the endoscopic scanner opens up new potential for more comprehensive optical imaging applications.
期刊介绍:
IEEE Photonics Technology Letters addresses all aspects of the IEEE Photonics Society Constitutional Field of Interest with emphasis on photonic/lightwave components and applications, laser physics and systems and laser/electro-optics technology. Examples of subject areas for the above areas of concentration are integrated optic and optoelectronic devices, high-power laser arrays (e.g. diode, CO2), free electron lasers, solid, state lasers, laser materials'' interactions and femtosecond laser techniques. The letters journal publishes engineering, applied physics and physics oriented papers. Emphasis is on rapid publication of timely manuscripts. A goal is to provide a focal point of quality engineering-oriented papers in the electro-optics field not found in other rapid-publication journals.